Replication Of DNA

Question 1

State 4 things that must be present for DNA replication.

Answer 1

Primers
DNA Polymerase
4 Nucleotides
Ligase

Question 2

Describe the stages in DNA replication.

Answer 2

Hydrogen bond breaks and the DNA unzips
A primer is added to the start of the piece of DNA being copied
DNA polymerase adds in nucleotides at the 3’ side
This is a continuous process

Question 3

Describe what a primer is and explain its role in DNA.

Answer 3

Short strands of RNA that initiates DNS synthesis.

Primers are what bonds to the DNA to make copies of it.

Question 4

Name 2 enzymes involved in DNA replication.

Answer 4

Helicase and Topoisomerase

Question 5

Describe the purpose of PCR

Answer 5

Allows specific sections of DNA to be amplified into vitro

Question 6

Explain what is involved in the ‘thermal cycling’ of PCR.

Answer 6

a process of heating and cooling that creates the conditions necessary for DNA replication. … This heats the DNA sample to a point where the double-stranded sample becomes uncoupled to create two individual DNA strands