Replication_Lecture1 - Manel Camps

Question 1

oriC

Answer 1

Chromosomal region where replication initiates.

Question 2

DnaA

Answer 2

AAA ATPase protein that oligomerizes at the bacterial origin and unwinds DNA to initiate replication.

Question 3

AAA ATPase

Answer 3

Family of proteins that can bind either ATP or ADP, act as ATPases; usually more active in the ATP-bound form.

Question 4

Consensus Sequence

Answer 4

the ideal DNA sequence that a protein interacts with.

Question 5

DNA Unwinding Element (DUE)

Answer 5

A specific sequence in oriC that is unwound by DnaA.

Question 6

Temperature Sensitive

Answer 6

A type of mutation that displays a wild-type phenotype at a low temperature (e.g. 30C) but a mutant phenotype at a higher temperature (e.g. 42C).

Question 7

FIS (Factor for Inversion Stabilization)

Answer 7

DNA binding protein that bends DNA; blocks DnaA and IHF in oriC.

Question 8

IHF (Integration Host Factor)

Answer 8

DNA binding protein that bends DNA

Question 9

SeqA

Answer 9

DNA binding protein that bind hemimethylated GATC sequences.

Question 10

RIDA (Regulatory Inactivation of DnaA)

Answer 10

Activity of replisome to stimulate DnaA ATPase activity, thus converting DnaA-ATP to DnaA-ADP. Conferred by Hda protein.

Question 11

DnaA titration

Answer 11

A mechanism for sequestering DnaA-ATP, by having it bind to non-oriC sequences throughout the chromosome.

Question 12

DARS

Answer 12

DNA sequences that bind DnaA and stimulate it to release ADP; apo-DNA then acquires an ATP.