Replication_Lecture2 - Manel Camps Flashcards
(10 cards)
1
Q
Pol III core
A
DNA polymerase, 3’ to 5’ exonuclease
2
Q
Sliding clamp
A
Processivity factor
3
Q
Clamp Loader
A
Assembles sliding clamps, protein trafficking
4
Q
DnaB helicase
A
5’-3’ DNA unwinding
5
Q
Primase
A
Synthesizes RNA primer, DnaG/RNA Pol
6
Q
SSB
A
Binds to SS DNA, prevents secondary structure
7
Q
RNAse H
A
removes RNA primer, allowing DNA Pol I to finish synthesis of new DNA.
8
Q
DNA Pol I
A
synthesizes DNA where RNA primer was and removes rest of primer
9
Q
DNA Ligase
A
covalently attaches pieces of DNA
10
Q
Gyrase
A
Keeps DNA ahead of the fork from overwinding
