revision - nutrition + enzymes feb 2023 Flashcards
chemical elements in carbohydrates
carbon, hydrogen + oxygen
chemical elements in protein
all have hydrogen, nitrogen, carbon + oxygen
most have sulfur
chemical elements in lipids
all contain carbon, hydrogen + oxygen
some have nitrogen, sulfur and phosphorus
describe the structure of carbs, proteins and lipids
larger molecules made from smaller, basic units
building blocks of carbs
made from starch and glycogen (polysaccharides), which are made from simple sugars (monosaccharides like glucose and fructose)
building blocks of protein
amino acids
building blocks of lipids
fatty acids and glycerol
test used for detecting glucose
benedict’s solution - orange/brick red if there is, blue, greeny-yellow if there isn’t much (requires heating to 80c)
test used for detecting protein
biurets solution - blue = none, lilac = has protein
test used for detecting lipids
emulsion test - add ethanol and water to solution and shake. milky white in presence of lipids
test used for detecting starch
iodine test - add iodine (blue/black if there’s starch, stays brown/orange if there isn’t)
functions of enzymes
biological catalysts in metabolic reactions, speeding up the reactions without really being affected itself
how temperature effects enzymes
Enzymes have an optimum temperature which is the temperature where the enzyme works the most effectively. enzymes aren’t living so they can’t die. but when enzymes exceed their optimum temperature, the shape of their active site changes, meaning the lock and key mechanism no longer works. we say that the enzyme is denatured
experiment with enzymes and temperature
iodine test for starch, heating enzymes and solution to different temperatures (use amalyse and starch)
what food groups would a balanced diet contain
carbs, proteins, lipids, vitamins, minerals, water, dietary fibre