RHEUMATOID FACTOR

Question 1

RHEUMATOID ARTHRITIS IS A

Answer 1

SYSTEMIC AUTOIMMUNE DISORDER

Question 2

THE PREVALENCE OF DISEASE IS HIGHEST IN

Answer 2

WOMEN MORE THAN 65 YEARS OF AGE

Question 3

RHEUMATOID ARTHRITIS IS CHARACTERIZED BY (3)

Answer 3

CHRONIC

SYSTEMIC

EROSIVE ARTHRITIS OF PERIPHERAL JOINTS

Question 4

RHEUMATOID ARTHRITIS ALSO AFFECTS WHAT ORGAN (2)

Answer 4

HEART

LUNGS

Question 5

STRONGEST ASSOCIATION HAVE BEEN BETWEEN A SUBSET OF

Answer 5

PATIENTS WITH RA AND HLA-DRB1 or PTPN22 GENE POLYMORPHISMS

Question 6

STRONGEST ENVIRONMENTAL RISK FACTOR FOR RA

Answer 6

CIGARETTE SMOKING

Question 7

PATHOLOGY OF RA RESULTS IN

Answer 7

DESTRUCTION OF BONE & CARTILAGE

Question 8

LESIONS IN RHEUMATOID JOINTS SHOW AN INCREASE IN CELLS LINING THE

Answer 8

SYNOVIAL MEMBRANE

FORMATION OF PANNUS

Question 9

INFILTRATION OF THE INFLAMED SYNOVIUM WITH (5)_____ IS EVIDENCE OF IMMUNOLOGIC ACTIVITY WITHIN THE JOINT

Answer 9

T AND B LYMPHOCYTES

PLASMA CELLS

DENDRITIC CELLS

MAST CELLS

GRANULOCYTES

Question 10

RHEUMATOID FACTOR IS AN ANTIBODY THAT IS MOST OFTEN OF THE ___ AND IS DIRECTED AGAINST THE FC PORTION OF ______

Answer 10

IgM CLASS

IgG

Question 11

IN RA, ANTIBODIES SUCH AS _______AND M______ ARE THOUGHT TO COMBINE WITH THEIR SPECIFIED ANTIGEN AND RESULTING IMMUNE COMPLEXES BECOME DEPOSITED IN THE JOINTS RESULTING IN A _______

Answer 11

RHEUMATOID FACTOR

ANTI-CCP

TYPE 3 HYPERSENSITIVITY REACTION

Question 12

THE COMPLEMENT PROTEIN ___ BINDS TO THE IMMUNE COMPLEXES ACTIVATING THE CLASSICAL COMPLEMENT CASCADE. DURING THIS PROCESS ___ And ____ ARE GENERATED WHICH ACTS AS ______ FOR ______AND ______.

Answer 12

C1

C3a AND C5a

CHEMOTACTIC FACTORS

NEUTROPHILS AND MACROPHAGES

Question 13

RF CAN BE FOUND IN PATIENTS WITH OTHER CONNECTIVE TISSUE DISEASES (5)

Answer 13

SYSTEMIC LUPUS ERYTHEMATOSUS

SJOGREN’S SYNDROME

MIXED CONNECTIVE TISSUE

PEOPLE WITH CHRONIC INFECTIONS

Question 14

MANUAL AGGLUTINATION TEST USING _____ OR ______HAVE BEEN USED FOR MANY YEARS TO DETECT _____

Answer 14

CHARCOAL OR LATEX PARTICLES COATED WITH IgG

RHEUMATOID FACTOR (RF)

Question 15

MANUAL AGGLUTINATION TEST ONLY DETECTS THE ______

Answer 15

IgM ISOTYPE

Question 16

MANUAL AGGLUTINATION TEST IS REPLACED BY____ (3) WHICH ARE AUTOMATED, HAVE GREATER PRECISION AND SENSITIVITY AND CAN ALSO DETECT OTHER RF ISOTYPES

Answer 16

ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)

CHEMILUMINESCENCE IMMUNOASSAY

NEPHELOMETRIC METHODS

Question 17

IS THE ANTIBODY THAT IS MOST OFTEN TESTED TO AID IN MAKING THE INITIAL DIAGNOSIS

Answer 17

RHEUMATOID FACTOR

Question 18

___%TO___% OF PATIENTS WITH RA ARE POSITIVE IN RF

Answer 18

70% TO 90%

Question 19

ONCE A DIAGNOSIS OF RA IS MADE, THE MOST HELPFUL TEST FOR FOLLOWING THE PROGRESS OF THE DISEASE ARE (3)

Answer 19

MEASUREMENT OF ESR

MEASUREMENT OF CRP

MEASUREMENT OF COMPLEMENT COMPONENTS

Question 20

___AND ___ ARE ELEVATED AND THE LEVELS OF SERUM COMPLEMENT COMPONENTS ARE NORMAL OR INCREASED BECAUSE OF _______.

___ LEVELS CORRELATE WELL WITH DISEASE ACTIVITY

Answer 20

ESR & CRP

INCREASED ACUTE PHASE REACTIVITY

CRP

Question 21

MOST CLINICAL MARKERS FOR RHEUMATOID ARTHRITIS IS

Answer 21

RHEUMATOID FACTOR IN SERUM

Question 22

IS A TERM USED TO DESCRIBE A VARIETY OF ANTIBODIES OR IMMUNE COMPLEXES

Answer 22

RHEUMATOID FACTOR

Question 23

RF LATEX REAGENT

Answer 23

SUSPENSION OF UNIFORM POLYSTYRENE PARTICLES COATED WITH IgG IN GLYCINE BUFFER pH 8.2

Question 24

THE RF LATEX REAGENT SENSITIVITY HAS BEEN ADJUSTED TO DETECT A MINIMUM OF _______ OF RHEUMATOID FACTORS

Answer 24

80 IU/mL or above

Question 25

REAGENT SENSITIVITY IS STANDARDIZED WITH THE

Answer 25

WORLD HEALTH ORGANIZATION RF STANDARD

Question 26

RF POSITIVE CONTROL SERUM

Answer 26

A STABILIZED PREDILUTED HUMAN SERUM CONTAINING AT LEAST 8 IU/mL of RF

Question 27

RF NEGATIVE CONTROL SERUM

Answer 27

A STABILIZED PREDILUTED HUMAN SERUM CONTAINING LESS THAN 8 IU/mL of RF

Question 28

BUFFER USED IN THE TEST

Answer 28

GLYCINE-SALINE BUFFER (20X)

Question 29

PROCEDURE OF QUALITATIVE METHOD

Answer 29

1.) ALLOW EACH COMPONENT OF THE TEST KIT TO REACH ROOM TEMPERATURE. 2.) GENTLY SHAKE THE LATEX REAGENT TO DISPERSE THE PARTICLES. 3.) PLACE A DROP OF UNDILUTED SERUM INTO A CIRCLE OF A TEST SLIDE. 4.) ADD 1 DROP OF RF LATEX REAGENT NEXT TO THE DROP OF SERUM. 5.) SPREAD THE REAGENT AND SERUM SAMPLE OVER THE ENTIRE AREA OF THE TEST CIRCLE USING A SEPARATE STIRRER FOR EACH SAMPLE. 6.) GENTLY TILT THE TEST SLIDE BACKWARDS AND FORWARDS FOR 2 MINUTES. 7.) OBSERVE AND INTERPRET THE RESULTS.

Question 30

POSITIVE AND NEGATIVE CONTROLS SHOULD BE RUN AT

Answer 30

INTERVALS

Question 31

NEGATIVE RESULT OF QUALITATIVE METHOD

Answer 31

UNIFORM MILKY SUSPENSION WITH NO AGGLUTINATION OBSERVED WITH RF NEGATIVE CONTROL

Question 32

POSITIVE RESULT OF QUALITATIVE METHOD

Answer 32

AGGLUTINATION, THE SPECIMEN REACTION SHOULD BE COMPARED TO RF POSITVE AND NEGATIVE CONTROLS

Question 33

RESULTS SHOULD BE READ _____ AFTER THE MIXING THE REAGENT ON THE SLIDE

Answer 33

2 MINUTES

Question 34

CONDITIONS OF INCREASED LEVELS OF RF (4)

Answer 34

INFECTIOUS MONONUCLEOSIS SARCODOSIS LUPUS ERYTHEMATOSUS SJOGREN’S SYNDROME

Question 35

REAGENTS ARE STABLE WHEN STORED IN

Answer 35

2-8C, DO NOT FREEZE