Rodriguez: Gram (-) cocci Flashcards by JHOANNA LYNN LUMBOY

-gram negative DIPLOCOCCI
-COFFEE or KIDNEY BEAN shaped
-convex, glistening

neisseria

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neisseria are non pigmented except for

n. flava, n. flavescens, n. subflava

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all neisseria are
oxidase +
catalase + except

n. elongata

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-sensitive to drying, direct inoculation at bed side is required
-carbohydrate ferments: glucose and maltose

neisseria

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-never a normal flora
-man only known host
-spread through sexual contact
-mother to newborn
-LEADING CAUSE OF SEXUALLY TRANSMITTED DISEASE
-produce IgA protease
-has common pili

Neisseria gonorrheae/ gonococci

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an important pathogenesis of both n. gonorrheae and n. meningitidis

IgA protease

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N. gonorrheae

T1 and T2

virulent

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N. gonorrheae

T3-T5

avirulent

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rare isolates of n. gonorrheae that are isolated from asymptomatic men

auxotypes

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-flow of seed
-brothel
-acute pyogenic infection 2-7 days incubation
-symptom: purulent discharge

gonorrhea

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untreated gonococcal cervicitis may cause sterility and peri hepatitis known as

Fitz-Hugh-Curtis sydrome

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CA
purulent urethritis- males
cervicitis- females

n. gonorrheae

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the chief complain in symptomatic oropharyngeal infections

pharyngitis- n. gonorrheae

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also known as rectal gonorrhea causing rectal pain and bloody stools

anorectal infections, n. gonorrheae

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a gonococcal eye infection during vaginal delivery

conjunctivitis/ophthalmia neonatorum

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for collection of n. gonorrheae specimens

dacron/ rayon swabs

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transport medium for swabs of n. gonorrheae

amies medium with charcoal

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> 5PMNS/field but no bacteria

non gonococcal urethritis

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non gonococcal urethritis caused by

C. trachomatis

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swab of n. gonorrheae should be inoculated onto media in a – pattern

z pattern, cross streaked

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best method for culture and transport should contain CO2 of n. gonorrheae

bottle or chamber containing CO2

candle jar

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best method for culture and transport should contain CO2 of n. gonorrheae

citric acid-bicarbonate tablet

JEMBEC

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for n. gonorrheae

antimicrobial agents: vancomycin
colistin
nystatin

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thayer martin agar/ TMA

for n. gonorrheae

antimicrobial agents:
vancomycin
colistin
nystatin
trimethroprim lactate

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modified thayer martin agar/ MTM

for n. gonorrheae antimicrobial agents: vancomycin colistin anisomycin trimethrophrim lactate

martin lewis medium/ ML

for n. gonorrheae antimicrobial agents: vancomycin colistin trimethrophrim lactate amphotecirin B

new york city medium/ NYC

has an advantage of detecting genital mycoplasma

new york city medium/ NYC

for n. gonorrheae antimicrobial agents: vancomycin colistin trimethrophrim lactate amphotecirin B lincomycin

GC- LECT medium

transgrow cary-blair amies JEMBEC

transport medium n. gonorrheae

-standard method of identifying n. gonorrheae -detects producion from glucose, maltose, lactose, fructose, sucrose

CHO utilization test

DNAse test in gram - cocci m.catarrhalis

DNAse test in gram - cocci n. gonorrheae

highly specific and sensitive, confirms morphologic appearance of bacteria

fluorescent antibody test (FAT)

confirms biochemical identification

coagglutination

suitable for screening many patients for n. gonorrheae

molecular assays

used for culture confirmation or direct detection in swab specimens direct detection of gonococcal ribosomal RNA

chemiluminescent nucleic acid probe

-causative agent of epidemic meningococcal meningitis/meningococcemia/cerebrospinal fever/ SPOTTED FEVER

neisseria meningitidis/ meningococci

-encapsulated with HALO -blusih gray colonies -produce IgA protease

n. meningitidis/ meningococci

presence of n. meningitidis in the blood and can occur as an acute or chronic form symptom: stiff neck

meningococcemia

may develop during bacteremic spread due to release of endotoxin

petechial skin lesions

activates the clotting cascade producing waterhouse-friderichsen

LOS-endotoxin

hemorrhage in the adrenals caused by n. meningitidis

waterhouse-friderichsen syndrome

individuals with deficiency in complement -- are at risk of meningococcemia

C5-C8

drug of choice for treatment of meningococcal meningitis

penicillin G

resembles N. meningitidis on gonococcal selective media

neisseria lactamica

-similar to T3 colonies of n. gonorrhea on CAP

n. cinerea

will differentiate n. cinerea from n. gonorrheae

colistin susceptibility

yellow pigmented neisseri, assacharolytic

n. flavescens

most commonly found in nasopharynx of infants and children's 2 years of age neisseria

n. lactimica

large, very mucoid colonies seen in nasopharynx of children and young adults also from airways of dolphin neisseria

n. mucosa

dry wrinkled breadcrumbs like colonies neisseria

n. sicca

rod shaped weakly positive or negative catalase test neisseria

n. elongata

rod shaped normal oral microbiota in dogs

n. weaveri

specie that resembles neisseria by exhibiting gram negative coccal morphology

m. catarrhalis aka. branhamella catarrhalis

-3rd most common cause of otitis media and sinusitis in children -adjacent sides are flattened -HOCKEY PUCK appearance

moraxella catarrhalis aka. branhamella catarrhalis

Rodriguez: Gram (-) cocci Flashcards

(55 cards)