SEMR only: QC and QA Flashcards by JHOANNA LYNN LUMBOY

Q

Pre-analytical phase
* Test requisition
* Processing of test requisition
* Collection of sample
* Sample transport
* Initial processing
* Transport to microbiology section

A

QUALITY ASSURANCE

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Q

Analytical phase
* Examination and workup of culture
* Interpretation of results

A

QUALITY ASSURANCE

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Q

Post- analytical phase
* Preparation and production of written report
* Distribution of results to the clinician or pickup by
patient or representative
* Intervention

A

QUALITY ASSURANCE

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Q

-Should be done and well documented for record
keeping

A

QUALITY CONTROL

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Q

Sample Details
* Proper and detailed request form
* Test scope and limitations
* Patient selection
* Optimal sample collection time
* Acceptable
* Appropriate transport medium
* Sample storage instructions
* Test availability

A

QUALITY CONTROL

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Q

should review and sign the MOP annually,

A

microbiology dept. supervisor / Bacteriology section
head

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Q

RAPID, SENSITIVE and SPECIFIC

A

Diagnostic test

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Q

TEMPERATURE
Incubator and Refrigerator thermometers
* Permanently immersed in –: prevents
temperature fluctuations every time the
door is opened.

A

glycerol

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Q

Equipment (Preventive maintenance)

incubator

A

daily

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Q

Equipment (Preventive maintenance)

anaerobic chamber

A

daily

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Q

Equipment (Preventive maintenance)

BSC

A

annually, anytime hoods are removed

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Q

Equipment (Preventive maintenance)

centrifuge, microscope

A

every 6mos, or as needed
4x a year

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Q

Equipment (Preventive maintenance)

autoclave

A

each load

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Q

Equipment (Preventive maintenance)

balance

A

anually

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Q

Most bacteria grow in pH – while
animal cells thrive in pH 7.2 – 7.4.

A

6.5 - 7.0

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Q

Most bacteria grow in pH 6.5 - 7.0 while
animal cells thrive in pH –

A

7.2 – 7.4.

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Q

Media preparation

plated

A

1 month

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Q

Media preparation

tubed

A

6 months

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Q

reagent - if an organism produces
acetylmethyl carbinol from glucose fermentation

A

Voges-Proskauer

Q

paper disks impregnated with growth
factors

A

X and V strips

Q

SUSCEPTIBILITY TESTING
Agar depth

A

Kirby Bauer method

Q

SUSCEPTIBILITY TESTING
Evaporation

A

Microtiter dilution

Q

organism:

monitoring of Ca, Mg

A

P. aeruginosa

Q

organism:

monitoring thymidine

A

e. faecalis

organism: for gram + antibiotics

staph aureus

organism: for gram - antibiotics

e. coli

lowest concentration of antibiotic that can inhibit organism

MIC- minimum inhibitory concentration

Increasing -- concentration predisposes P. aeruginosa in resistance to aminoglycosides

calcium and magnesium

Increasing -- concentrations will cause false resistance to drugs such as: sulfonamides, trimethoprim, trimethoprim sulfamethoxazole

thymidine

a private or non-profit global biological resource center which standardize different organisms and strains

ATCC American type culture collection

Organisms should be subcultured -- after thawing to regain its healthy state

twice

media for freezing should not contain -- as the acid produced may kill the organism in time.

sugars

Preferred media for stock cultures: optimum media for anaerobes

Chopped meat

Preferred media for stock cultures: for fungi

Potato dextrose agar

Preferred media for stock cultures: for viruses

Fetal bovine serum with 10% dimethyl sulfoxide

states that employee competency must be determined and verified upon employment.

CLIA 1988

Frozen non fastidious organisms – thawed , recultured and refrozen every--

5 years

Frozen fastidious organisms – thawed , recultured and refrozen every--

3 years

sends” blind unknowns”

EQAS or NEQAS

* This serves to establish the accuracy and reproducibility of laboratory’s everyday performance

PROFICIENCY TESTING

* This serves to establish the accuracy and reproducibility of laboratory’s everyday performance

PROFICIENCY TESTING

NRL: CC

Lung center of the Philippines-

NRL: Drug testing

East avenue

NRL: Hematology

National kidney transplant institute-

NRL: HIV/ Aids.

San Lazaro

NRL: * Bacteriology * Parasitology * Mycobacteriology * Transfusion Transmissible Infections * SARS-CoV-2 Molecular

Research institute of Tropical Medicine

All QC records should be maintained for at least --except for equipment documentation.

2 years