Seminal Fluid Analysis Flashcards Preview

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Flashcards in Seminal Fluid Analysis Deck (24)
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1

purpose for seminal fluid analysis

evaluate infertility
post vasectomy

2

cells that produce sperm

Sertoli cells in seminiferous tubules of testes

3

semen components

fructose, flavin, proteins, prostaglandins, citric acid, zinc, enzymes (ACID PHOSPHATASE)

4

Bulbourethral glands

secrete fluid that lubricates the penis & neutralizes urethra

5

Testes & epididymis

sperm produced in testes & mature in epididymis

6

Prostate

FIRST PORTION OF EJACULATE- thin milky fluid
contains most of the sperm!!!!
acidic
contains acid phosphatase & proteolytic enzymes resulting in liquefaction

7

Seminal vesicles

LAST FRACTION OF EJACULATE
major contributor of volume
alkaline
enzymes necessary for coagulation
supplies fructose, flavin & other nutrients

8

collection

several samples over 3 month period
2-7 days abstinence
deliver to lab within one hour maintained at body temp

9

appearance

normal: homogeneous, gray-white, opalescent
abnormal: clear, red, yellow green etc

10

normal coag & liquefaction

completely liquefied within 60 minutes at room temp
can treat with chymotrypsin if not completely liquefied

11

normal volume

>1.5 ml

12

normal viscosity

semen falls from pipet in drops or threads are

13

normal pH

>7.2

14

normal motility

50% or more of sperm should show moderate to strong linear or forward progression
>32% progressively motile (!!!!!!!!!)

15

Sperm concentration

dilute specimen & perform count on hemocytometer
normal: 15-150 mil/ml

16

calculation for sperm concentration

# cells x dilution
-----------------------x 1000
volume

17

total sperm count

sperm concentration x total volume
normal > 39x10^6 / ml

18

stains

Wright, Giemsa, Papanicolauo stains

19

morphology

stain use 100x oil, observe 200 sperm
normal >4% normal morphology

20

head

oval
2.5-3.5 um wide & 4.0-5.0 um long
head to length ratio should be 1.5-1.75

21

midpiece

6-7.5 um long & thicker than tail but

22

tail

slender, uncoiled @ least 45 um long

23

sperm vitality

done when motility

24

agglutination

even small amount is abnormal
production of anti-sperm antibodies
clumping = bad