Cerebrospinal fluid analysis Flashcards Preview

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Flashcards in Cerebrospinal fluid analysis Deck (38)
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1

Dura mater

outer membrane of brain

2

arachnoid mater

middle membrane of brain

3

pia mater

inner membrane

4

choroid plexus cells

in the center of the brain area
produces cerebrospinal fluid

5

composition of cerebrospinal fluid

selective secretion from plasma NOT an ultrafiltrate

6

collection

3+ sterile tubes
1: chemical & immunological testing
2: microbiology
3: cell count & cytology
4: optional, micro

7

crystal clear color

normal cerebrospinal fluid

8

cloud or turbid color

wbcs, increased protein or lipid concentration

9

milky color

wbcs, increased protein or lipid concentration

10

xanthrochromic color

pink to orange color
rbcs degradation
increased serum bilirubin
carotene
markedly increased protein
melanoma pigment

11

hemolyzed/bloody color

intracranial hemorrhage or traumatic tap

12

traumatic tap

greatest amount of blood in tube 1 & least in tube 3
after centrifugation , colorless supernatant
may see clots present in tubes bc of fibrinogen

13

hemorrhage

consistent amount of blood in all three tubes
after centrifuge, xanthochromic supernatant
macrophages with phagocytized RBcs

14

routine tests on CSF

cell count & differential
protein
glucose

15

temps for tube storages

1: can be frozen if needed
2: micro- room temp
3: refrigerated for cell counts

16

traumatic tap & wbcs

correct for contamination

17

D-dimer for hemorrhage & traumatic tap

hemorrhage: positive D-dimer
traumatic tap: negative D-dimer

18

range for RBCs in CSF

0 rbcs/ul for all ages

19

cytospin

pro: good cellular recovery & preservation; rapid & simple
con: production of some aberrant cells

20

cytocentrifugation

particle sedimentation speed increases w/ the rotational speed of the centrifuge; it is faster for large or dense particles & slower for small or light particles

21

2 categories of cytocentrifugation

1. remove the suspension fluid during cell sedimentation
2. retain the suspension fluid during cell sedimentation

22

normal adult CSF differential

lymphs & monos = 70:30
few neutrophils
few lining cells & macrophages

23

normal children CSF differential

monos > lymphs
ependymal cells & macrophage

24

neutrophilia

bacterial meningitis

25

macrophages present

recent hemorrhage usually
appear 2-4 hours after RBC introduction

26

protein

usually determined to assess the integrity of the blood-brain barrier
also used to indicate CNS pathology
15-45 mg/dl

27

increased protein

decreased reabsorption into the venous blood
increased synthesis of immunoglobulin
contamination w/ blood during tap

28

decreased protein

increased reabsorption by the arachnoid villi due to increased intracranial pressure
fluid leaking !!

29

CSF/ serum albumin index

used to evaluate the integrity of blood-brain barrier

30

CSF IgG index

used to measure IgG synthesis w/in the CNS
multiple sclerosis >0.70