Sensors Flashcards

Sensors (275 cards)

1
Q

What is a wave?

A

Field disturbances that transfer energy from one location to another

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2
Q

What is a detector?

A

devices used to measure the characteristics of field disturbances

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3
Q

What do characteristics of disturbances reveal?

A

information about a waves origin

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4
Q

What can waves carry?

A

information from one location to another which is accessed by detection

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5
Q

What can EM radiation tell us about stars?

A

material composition

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6
Q

define passive sensing system

A

a system that generates a signal in response to a stimulus under normal environmental conditions

eg retina, some metal detectors

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7
Q

Define an active detection system

A

a system where the sensor requires a non natural stimuli to generate a signal, eg x ray system, MRI

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8
Q

Describe active detection in a cyber context

A

the sensor(s) uses a digital probe signal to instigate a response from a target, which is then measured. Requires more computing power and potentially less susceptible to data poisoning.

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9
Q

Describe passive detection in a cyber context

A

the sensor(s) measure ambient digital signals only, without the use of a probe on the target. Can be more susceptible to data poisoning than active measures

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10
Q

Describe binary classifiers

A

most detection cases we care about binary classification eg on/off, high/low, moving/stationary in a security context - threat/non threat

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11
Q

Describe a confusion matrix

A

red/green matrix, at simplest a 4 square grid
X axis - signal/actual/object
Y axis - detector response/predicted values
Green is real positives/negatives
Red is false positives/negatives

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12
Q

What is the X axis of a confusion matrix?

A

X axis - signal/actual/object

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13
Q

What is the Y axis of a confusion matrix?

A

Y axis - detector response/predicted values

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14
Q

What are the green regions of a confusion matrix?

A

Green is real positives/negatives

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15
Q

What are the red regions of a confusion matrix?

A

Red is false positives/negatives

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16
Q

On a 4 square confusion matrix, what are the 4 binary classifiers?

A

True positive - TP
True negative - TN
False positive - FP
False negative - FN

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17
Q

What are the 5 base calculations on a confusion matrix that help define how good a detector is?

A

Total positive = TP + FN
Total negative = FP + TN
Total true readings = TP + TN
Total false readings = FP + FN
Total readings = TP + TN + FP + FN

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18
Q

how to calculate accuracy on a confusion matrix

A

Accuracy = total positive / total measurements

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19
Q

What is specificity of a detector?

A

Specificity is the true positive rate

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20
Q

How do you calculate specificity?

A

True positive rate = TP / (TP + FN)

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21
Q

What is sensitivity of a detector?

A

true negative rate

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22
Q

how do you calculate sensitivity?

A

true negative rate = TN / (FP + TN)

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23
Q

What measure indicates that a detector will alarms correctly?

A

Specificity or true positive rate

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24
Q

What gives the probability that a detector will alarm incorrectly?

A

Incorrect alarm rate = 1 - true negative rate

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25
Define a ROC curve?
Receiver Operator Characteristic curve is a way of plotting all possible confusion matrices to make it easy to identify the best threshold to make a decision.
26
define the axis of a ROC graph
X - False positive rate (1 - sensitivity ) Y - true positive rate (specificity )
27
What is 1, 1 on an ROC curve?
no true or false negatives
28
What does going up the Y axis on the ROC curve mean?
increased probability of detection
29
What does going up the x axis mean?
increase in the probability of false alarm
30
What is AUC?
Area under the curve
31
What is ROC sensitivity index?
d' or D prime is the distance a ROC curve is from the 0,0 1,1 graph. in general, the further from the 0,0 1,1 graph the better the sensor is, but this will be dependant on risk appetite for false alarms.
32
4 particulate sampling techniques
Filters impactors dry cyclones wetted wall cyclones
33
3 gas sampling techniques
Absorption Adsorption Condensation
34
4 key air sampling questions
Why are you sampling what are you sampling where are you sampling how long are you sampling for
35
7 main analytes
aural biochem magnetic optical radiological thermal
36
define an analyte
a substance who's chemical constituents are being identified and measured
37
what is the prerequisite for detection of a stimulus?
must be present in sufficient CONCENTRATION or INTENSITY so it can be DETECTED and DIFFERENTIATED from the environment
38
4 routes of entry to the human body
respiratory percutaneous ocular ingestions
39
2 features of the air we breathe
Highly diverse Ambient aerosol
40
Define highly diverse
already contains bacteria, viruses and toxins infections are from an aerosol route
41
Define ambient aeresol
contains naturally occurring aerosolised particles physically identical to threat particles present at continually fluctuating levels
42
6 particulate sampling techniques
filtration impingement/impaction sedimentation precipitation thermal electrostatic
43
4 stages of air sampling
pre-separator filter/collection media airflow controller pump
44
Features of PM10 high volume air sampler
Flow rate between 1-1.7 m^3/min size selective inlet allow for particles of pre determined range to be captured on a quartz filter (usually less than 10 microns and filter paper determines the minimum size) filter weighted before and after use to determine the mass of particulates caught controlled by mass flow controller or volumetric flow controller
45
types of filter (8)
glass fibre quartz fibre borosilicate fibrecellulose and mixed cellulose esters (MCE) PTFE PM 2.5 Glass fibre tape Andersen impactor filters Polycarbonate filter
46
Concentration calculation
concentration = mass of pollutant / volume of air
47
gravimetric concentration formula
SP = (Wf-Wi)/V(T) x 10^6SP = mass concentration of suspended particulate Wf = final weight of filter Wi = initial volume of filter V(T) = total volume of air sampled 10^6 = conversion of g to ug
48
Define aerodynamic diameter of a particle
a sphere, whose density is 1 g cm^-3 which settles in still air at the same velocity as the particle in question
49
Define Mass Median Aerodynamic Diameter
is defined as the diameter at which 50% of the particles by mass are larger and 50% are smaller.
50
Define Geometric Standard Deviation (GSD)
is a measure of the spread of an aerodynamic particlesize distribution. Typically calculated as follows:GSD = (d84 /d16)^1/2where d84 and d16 represent the diameters at which 84% and 16% of the aerosol mass are contained, respectively, in diameters less than these diameters.
51
Types of air sampler (*)
Cyclone3 piece cassette swirling aerosol collector AGI 30 Slit sampler Andersen sampler
52
3 categories of materials for sensing
Structural, functional, biomaterials
53
Define structural material
Structural materials are used for their structural integrity and mechanical behaviour. Strength, weight, toughness, hardness…
54
Define functional material
Functional materials are used for their function or response to a stimulus e.g. magnetic , electrical, optical
55
Define biomaterials
materials that have been designed to interface with biological systems, for the treatment, augmentation, or replacement of biological functions. Biomaterials and biological systems interact both ways.
56
% categories of materials
metal, ceramic, polymer, glass, composite
57
Triangle of material choice
processing Properties Structure
58
Define processing of materials
the journey from the ground to a usable material in a system ore - purify
59
What is the difference between detect to treat and detect to warn?
Detect to warn systems must respond in sufficient time to allow protective measures to prevent or minimize exposure of a significant portion of the at risk population. Detect to treat is much longer as you accept people will be affected, you look to mitigating the severity of the exposure
60
What is detect to treat?
Detect to treat is much longer as you accept people will be affected, you look to mitigating the severity of the exposure
61
what is detect to warn?
Detect to warn systems must respond in sufficient time to allow protective measures to prevent or minimize exposure of a significant portion of the at risk population.
62
Detection strategy for detect to treat
Point sensing
63
Detection strategy for detect to warn
Stand-off sensing Eg LiDAR
64
What do we mean by generic detection?
detection of a range of threats ie wide groups of bacteria which a small group might be a threat agent
65
What analytical systems provide generic detection
LIDAR
66
what is flow cytometer?
pass a cell suspension through a light source and measure the fluorescence from the agents
67
What do we mean by structural sensing?
detection of a threat by use of the structural elements of the threat, usually from binding to part of these elements with something that is easy to detect e.g. an agglutination test
68
What are the five bio-analysis methods?
Culture Microscopy Immunoassay PCR Chemical Assays
69
Describe Method of Bioanalysis Culture
grow agents in conditions that specifically relate certain types of bacteria/fungi etc ie rose Bengal agar is a growing media that inhibits bacteria and allows the identification of the presence of yeast and moulds
70
Describe Method of Bioanalysis Microscopy
Morphological identification of particles
71
Describe Method of Bioanalysis Immunoassay
particles with specific epitopes matching the assay antibodiesparticles with parts of the allergen that are identified by the body (epitodes) that in this case match the antibodies present in the assay test.
72
Describe Method of Bioanalysis PCR
DNA matching test
73
Describe Method of Bioanalysis chemical assays
identify the biomass of specific chemicals eg ATP
74
Limitations of bioanalysis method culture
underestimates concentration of all organisms nonculturable organisms are invisible non culturable are classed as noninfective
75
Limitations of bioanalysis method microscopy
limited to groups of organisms, not specific strains
76
Limitations of bioanalysis method immunoassay
limited to organisms that the assay is designed for, therefore only binary resultsCross reactivity is common
77
Limitations of bioanalysis method PCR
limited to organisms that the assay is designed for, therefore only binary results highly specific highly sensitive
78
Limitations of bioanalysis method Chemical analysis
only an indicator for large quantities of organisms
79
what is meant by direct binding event?
direct binding of the target to a specific molecular recognition element
80
how does direct binding event works?
a reversable 'lock and key' event like an antibody with a viral protein
81
9 molecular recognition elements for biosensing
single strand DNA Antibody Peptide Enzyme Lectine Receptor Aptamer Small molecule Imprinted molecule
82
What is an antibody?
also known as an immunoglobulin Ig is a large, Y shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
83
How do immunoassay ticket systems work?
liquid sample added to test strip and other reagents added if required target molecules wick through the ticket, bind to immobilized agents and detection molecules in a 'sandwich' format wick usually travels through a testing area before reaching a control line, if no line shows up on control after allotted time, then the test needs repeating.
84
Define SELEX?
Systematic Evolution of Ligands by EXponential Enrichment a method of increasing the number of DNA/RNA strands required for testing and use them to sequence and characterise DNA found in the environment The starting single stranded DNA or RNA library(10 14 ~10 16 random oligonucleotides) is composed of sequences 20~100 nucleotides in length with a random region in the middle flanked by fixed primer sequences. After incubation with the target of interest, the bound oligonucleotides are partitioned from unbound sequences and amplified by PCR . repeated 2-15 times before used as biomarker identification tools.The resulting enriched DNA pool is used for the next round of selection.
85
What is SELEX short for?
Systematic Evolution of Ligands by EXponential Enrichment
86
6 high level approaches for structural sensing
Magnetic optical electrochemical mass acoustic/piezoelectric MEMS
87
Types of optical
Fluorescence Absorbance SPR/RM Luminescence RAMAN
88
Types of magnetic
magneto-elastic giant magneto-resistance (GMR)
89
types of MEMS (*)
MEOMScantileversmicrofluidsmicrocalorimetry
90
types of acoustic/piezo-electric
surface wave acousticquartz microbalance
91
types of mass
time of flight ion trap ion mobility (MS/MS)
92
types of electrochemical
amperometry potentiometric conductimetric molecular electronics
93
What is a molecular recognition element?
Molecular recognition event is typically a specific interaction that is reversible, analogous to the interaction between a lock and a key, although in many cases the binding would more accurately be described as induced fit, during which the recognition element changes shape upon binding.
94
What does a direct binding event rely on?
Affinity of the target for the molecular recognition elements Non-specific binding of extraneous material at the binding site Sensitivity of detection If attomolar (10 18 ) detection levels are required then high affinity molecular recognition elements with minimal non specific binding required
95
considerations for structure based sensing?
Sample collection, Sample concentration, Binding of the target to the molecular recognition element, Possible addition and removal of "reporter" groups, Detection of target molecular recognition element complex, Analysis of the output signal, Renewal of the sensor surface for repeated monitoring.
96
target inhibitor for single strand DNA?
complementary sequence of DNA
97
target inhibitor for Antibody
proteins, carbohydrates, small organic molecules etc
98
target inhibitor for Peptide
proteins, carbohydrates, small organic molecules etc
99
target inhibitor for enzyme
substrate such as biochemicals like glucose, acetic acid
100
target inhibitor for lectin
carbohydrate
101
target inhibitor for receptor
proteins, carbohydrates, small organic molecules
102
target inhibitor for aptamer
proteins, carbohydrates, small organic molecules etc
103
target inhibitor for small molecules
proteins, cells etc
104
target inhibitor for imprinted moelcules
proteins, small organics molecules, whole cells etc
105
8 SELEX methods
IP-SELEX Capture-SELEX Cell-SELEX CE-SELEX M-SELEX AFM-SELEX AEGIS-SELEX Animal-SELEX
106
Key aspects of IP-SELEX
includes immunoprecipitation
107
Key aspects of Capture-SELEX
oligonucleotide library is immobilized on a support instead of the targets to identify aptamers against small soluble molecules
108
Key aspects of Call-SELEX
utilizes whole live cells as targets for selection of aptamers
109
Key aspects of CE-SELEX
involes separation of ions based on electrophoretic mobility
110
Key aspects of M-SELEX
combines SELEX with a microfluid system
111
Key aspects of AFM-SELEX
employs AFM to create a 3D image of the sample surface
112
Key aspects of AEGIS-SELEX
utilizes libraries with the artificially expanded genetic code
113
Key aspects of animal-SELEX
aptamers are selected directly within live animal models
114
advantages of IP-SELEX
selects aptamers against proteins under normal physiological conditionsincreased affinity and specificity
115
advantages of Capture-SELEX
suitable for the selection of aptamers against small moleculesimmobilization of the target not requiredused for discovery of structure switching aptamers
116
advantages of Cell-SELEX
Prior knowledge of the target not requiredAptamers are selected against molecules in their native stateMany potential targets available on the cell surfaceProtein purification not required
117
advantages of CE-SELEX
Fastonly 1-4 rounds of selection requiredreduced non specific bindingtarget immobilization not required
118
advantages of M-SELEX
RapidVery effective (small amounts of reagents required)Applicable to small moleculesAutomatable
119
advantages of AFM-SELEX
able to isolate high affinity aptamersFast 3-4 rounds
120
advantages of AEGIS-SELEX
high specificity of the selected aptamers
121
advantages of animal-SELEX
selected aptamers bind the targets in their natural environmentPrior knowledge of target not requiredMinimal optimization needed
122
disadvantages of IP-SELEX
more time consuming than standard SELEX
123
disadvantages of Capture-SELEX
some oligonucleotides from the library might not be released/selected
124
disadvantages of Cell-SELEX
suitable for cell surface targetsRequires high level of technical expertiseCostlyTime consumingPost SELEX identification of target required
125
disadvantages of CE-SELEX
not suitable for small targetsexpensive equipment
126
disadvantages of M-SELEX
Not suitable low purity/recovery of aptamerstarget immobilization required
127
disadvantages of AFM-SELEX
expensive equipment requiredImmobilization of target aptamers required
128
disadvantages of AEGIS-SELEX
Poor recognition of the unnatural bases by natural DNA polymerases
129
disadvantages of Animal-SELEX
time consuming (man rounds required)
130
Dog’s nose; how does this compare with current detection systems?
test kits in the range of 1g-1ug (visible to particles)Field instruments in the range of 1ug-1pg (particles/vapour)Dogs in the range of 1fg-1ag (vapour)
131
Dog density of olfactory epithelium?
170cm^2(humans have 10cm^2)
132
How does the dog receptors mean it has a better sense of smell?
It is the interactions of odour molecules with specific receptor proteins that give specificity. Each olfactory odour receptor neuron has only one functional odour receptor; if the odour molecule interacts with the receptor then the nerve cell will respond.
133
Define nuclear forensics
the use of scientific techniques to investigate potentially criminal uses of radiological and/or nuclear material. most commonly used for counter proliferation purposes, but does have growing use in health and safety outside of civil nuclear too.
134
3 goals of nuclear security policies
–Development and control of military applications of nuclear energy Finding ways to curb the proliferation of states, or even non state actors, with nuclear capabilities; failing that Receiving best possible information on the nuclear programme or weapon arsenal of other states. a growing fourth area - reduce the use of RN material as a non monetary criminal currency
135
4 linkages that nuclear forensics can provide
people places materials events (including time components)
136
IAEA definition of nuclear forensics
Nuclear forensics is the examination of nuclear or other radioactive materials, or of evidence contaminated with radionuclides, in the context of legal proceedings under international or national law related to nuclear security
137
Types of radiation for detection
Alpha Beta Gamma Neutron Muon - not radioactive but part of high energy physics which most RN physicists are best placed to study this as well
138
Detection for alpha/beta
scintillator Signal analyser
139
types of detector for gamma
Gamma spectrometers consist of a (usually HPGe )detector used for measuring photon energies, apre amplifier, an amplifier, an analogue to digitalconverter (ADC ) and a multichannel analyser(MCA).
140
types of detector for neutron
He-3 gas with photomultiplier giving a count per unit time response
141
what is a neutron particle?
3 quarks, 1 up and 2 down which are generated in some nuclear decay chains and neutral in charge so very difficult to detect. present in the decay chains of plutonium and uranium which could indicate the presence of special nuclear material. this is useful for standoff detection.
142
what is and alpha particle?
a helium atom without the electrons, low travel distance and low penetration properties (sheet of paper will stop them)
143
what is an neutron particle?
3 quarks, 1 up and 2 down which are generated in some nuclear decay chains, most usefully the decay chains of plutonium and uranium which could indicate the presence of special nuclear material. this is useful for standoff detection.
144
what does a scintillator do?
converts energy lost by ionising radiation into pulses of light in solids or liquids which then interacts with a photocathode to give an electron multiplied in a Photo Multiplier Tube (PMT) to give electrical signal
145
What does the signal do?
analysed by a multi channel analyser and the intensity of each alpha particle energy is taken. it is the intensity at each energy level that allows an analyst to work out what types of radioactive material is present. noting there will probably be multiple elements in differing quantities so the decay chain is useful to cross reference with.
146
materials for scintillators
organic (plastic) or inorganic (ZnS or NaI)
147
how do you limit thermal noise?
electron hole production creates noise which can be mitigated by cooling in liquid nitrogen.
148
What are the axis of a gamma spectrum graph?
y axis - counts x axis - particle energy
149
What happens to the signal in a gamma spectrometer?
The preamplifier converts a charge pulse to avoltage pulse, while the amplifier provides avoltage gain . The ADC transforms the signal to adigital pulse that is used as input to the MCA. The MCA sorts the pulses according to their pulse height , which is proportional to the photoninteraction energy.
150
AWE objective for nuclear forensics
Develop and provide end to end operational response capabilities for detection, recovery, analysis, characterisation and technical attribution from all scenarios, exploiting nuclear and conventional forensics capabilities.
151
what does nuclear forensics + conventional forensics =
total forensic exploitation
152
Define nuclear characterisation
is the determination of a sample’s characteristics. It typically involves an elemental analysis of the sample, most often including isotopic analysis of nuclear materials (i.e. U and Pu) and selected minor constituents (e.g. Pb ). It also includes physical characterization e.g. measuring the key dimensions of solid samples, or for powders particle size and shape distribution.
153
Define nuclear reconstruction
is the process of combining the information from interpretation with all other available information (e.g. from forensic analysis of non nuclear evidence associated with the sample or from intelligence sources) to determine as full a history as possible of the RN material. This phase is called attribution in the contexts of investigations of nuclear trafficking and terrorism events.
154
what are the skills AWE have that make them import to the UK in nuclear security?
Hazardous materials of concern are used as part of AWE’s other core MOD programmes in support of the UK’s nuclear deterrent over the last 60 years. Unique knowledge base in AWE’s personnel and AWE’s facilities for characterisation of explosives, bulk SNM, radioactive materials. AWE provides key RN technical support to the UK CBRNE Operational Response
155
RN types of signature that can be assessed (*)
Shape Measurement markinggrain analysismicroscopychemical characteristics - composition of the uranium rare earth elemental pattern of the ore major, minor and trace constituents
156
describe point sensing
this is the processes of taking a sample of an analyte and putting it through a sensing capability
157
describe standoff sensing
the process of trying to identify a potential threat from a long way away
158
differences between point and standoff sensing
In point detection applications, the biological organisms must pass through the actual detection element. Standoff detection is detection of a biological agent cloud at some distance on the order of kilometres from the target and from the detector
159
What is LIDAR?
LIght, Detection And Ranging essentially a light illuminated radar system
160
uses of LIDAR in chem bio sensing
using a UV 297nm laser biological agents can be induced to fluoresce which can then be detected
161
other uses of LIDAR
navigation and positioning obstacle detection plant regrowth wind speed, turbulence etc (with doppler systems) speed guns 3d scanning
162
Define DIAL
Differential absorption lidar Allows for detection of aerosols and gases in atmosphere
163
Differences between LIDAR and DIAL
DIAL uses 2 lasers sources, one of the absorption band of interest, the other slightly outside this band and the difference in the intensities allow a concentration of molecule of interest to be calculatedLIDAR is single source and usually based on time of flight
164
Define LIBS
laser induced breakdown spectroscopy high power laser beam generates a plasma plume, the cooling plasma allowing elemental emission lines to be observed Very good for explosive identification
165
Point detection systems
DNA analysis PCR Gas chromatography Mass Spectrometry
166
Point sensing cons
ones based on aerosol particle counting with size generate unacceptable false positives unable to distinguish attack vs normal fluctuations in 1-10um range
167
Point sensing pros
size and shape analysis combined with UV-LIF is rapid and best used in a building environment due to controllable background noiseoxidation of organisms determined by NAD(P)H fluorescence is a potentially exploitable signature for near real time detection
168
Standoff detection cons
Long range IR LIDAR cannot distinguish between bio and non bio particles, so high false positive rate Operational systems need to employ temporal comparisons of spectral signatures to decrease false positive readings due to fluctuations in background signatures.
169
Standoff pros
Short range use of UV LIF provides near real time discrimination of biological from non biological particles. The ability to use shorter excitation wavelengths for short distances where atmospheric propagation is not an issue allows information containing spectral properties to be gathered from aerosolized particles
170
2 types of LIDAR (*)
direct energy detection (or incoherrent)coherent detection
171
steps in a LIDAR chain
laser transmitter scatter from targets recieving optics optical filter photodetector data acquisition
172
Define Nyquist-Shannon sampling theorem
“If we sample a signal at twice its highest frequency, then we can recover it exactly.” However, if the signal is sparse fewer samples are needed for reconstruction
173
Define Compressed sensing (CS) (or sparse sampling)
a novel paradigm in data acquisition that allows representing sparse data in an efficient and accurate way, using sparse recovery (SR) techniques based on nonlinear interpolation
174
What is the key idea of compressed sensing?
to recover a sparse signal from very few nonadaptive, linear measurements by convex optimization
175
Examples use of compressed sensing
Computed tomography image reconstruction
176
Steps in compressed sensing
1. Measure projections 2. First image estimate 3. simulated projections / corrected projections 4. comparison 5. correct images 6. iterative cycle - 3, 4, 5 7. end point - final images
177
What is machine learning?
study of computer algorithms that improve automatically through experience
178
What tasks are suitable for machine learning? (x3)
spam filtering pattern recognition data mining
179
conventional engineering design flow
acquisition of domain knowledge(physics based mathematical model) Algorithm development (algorithm with performance guarantees)
180
machine learning design flow
acquisition of data(training set) hypothesis class > learning(black box machine)
181
3 types of machine learning
supervised learning unsupervised learning (clustering) reinforcements learning
182
What is required for supervised learning?
input and output data are known
183
What is required for unsupervised learning?
input only is known
184
What is required for reinforcement learning?
input data and quality measure is known
185
what is an x-ray
high energy EM radiation essentially the same as gamma radiation Do not interact with matter much find it hard to pass through dense materials
186
formula for photon energy
plancks equation E = hv E= energy h = placks constant 6.626x10-34 J.s V = frequency in Hz
187
wavelength of x-rays
0.01-10nm hard x-rays are wavelengths under 0.2-0.1 nm
188
energy of hard x-rays
have energies above 5-10 KeV
189
4 parts of an Xray system
source target detector signal processing/detection algorithm
190
components of an X ray source
hot cathoderotating anode (around 10,000 rpm, heat generated approx. 2000C) tungsten target angled 12-15 degrees (though molybdenum or graphite can be used) Rotator
191
generation of xrays - how are K lines generated?
by high velocity electron from the anode knocking out an electron from the K (S) orbital and outer shell electrons dropping to lower energy states and releasing energy in the form of x rays as they do so.
192
generation of xrays - what are the characteristic interaction alpha and beta peaks created from?
alpha from 2p (outer L) shell to 1s (K) (smaller peak) beta from 3p (middle M) shell to 1s (K) (higher peak)
193
generation of xrays - what is bremsstrahlung radiation?
where a high energy electron passes close to a nucleus, it does not interact with the electrons, but is deflected by the nucleus, this deflection slows the electron and bremsstrahlung radiation is emitted with energy being proportional to how close to the necleus it was (the closer the higher the energy)
194
2 types of x ray detector/sensor
Scintillator direct converstion
195
define scintillator
these operate by converting packets of xray radiation into wavelengths which can then just be detected using CMOS/CCD devices that produce and electrical signals
196
define direct conversion (x-rays)
x-ray photons are directly converted to electrical signals
197
Pros of direct conversion over scintillator
better energy resolution better spatial resolution higher signal conversion efficiencies
198
steps of scintillator detection
Xray scintillator (xray converted to light, causes scattering) photodiodeelectric circuit (TFT or CMOS) image
199
steps of direct detection
xrayCdTe (xray converted to electrical signal, minimal scattering) CMOS circuit Image
200
Define CT scan
Computer tomography table moves through source/detectors and they move around target taking slices over time which are then used at the signal processing stage to build a 3d image
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Pros and cons of CT
pro 3d so can lead to better object identification can hold certain scans on file for checking for addition/subtraction of material comprehensive con higher dose rates, so may be unsuitable for circumstances where people might be exposed eg lorry backstakes a lot longer
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Define function based sensing
A function based detector is defined as a naturally occurring biological organism or portion of that organism (whether organ, tissue, cell, or receptor) that reacts in a measurable way when exposed to a range of chemical or biological toxic material. eg canary in coal mine
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differences between structure and function based sensing
Structure sensing is based in finding the agent that causes the problem Function based sensing is looking for evidence that the agent is present eg contrails/emissions to find planes
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Describe a cell based system (x2)
inherent system where natural processes are exploited for detection Engineered system, where a cell has been genetically engineered or had sensing materials added to them
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what is a hybrid system?
Hybrid systems exploit part of the functional process within a cell based response system, even as they are targeted at a specific characterized function.
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Describe an enzyme based chem detection system
NAIAD - nerve agent detection system The bioreceptor in this case is the enzymebutyrylcholinesterase , which exhibits the same enzyme activity as human acetylcholinesterase. The enzyme is immobilized onto a temperaturecontrolled pad that is continually washed with butyrylthiocholine methane sulfonate in an aqueousphosphate buffer. The enzyme catalyses the hydrolysis of the ester, producing butrylthiocholine , and the concentration is monitored by an electrochemical cell arrangement(this is the transduction system). If nerve agent is present, it inhibits the butyrylcholinesterase , which in turn causes an alteration in the electrical potential within the electrochemical cell, thus triggering an alarm at a present level.This type of detector is extremely sensitive to low levels of nerve agents such as tabun and sarin. Also HCN.
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Describe an ion based sensing system
, Competitive assay. Here a similar membrane is formed except that it contains hapten linked gramicidin, ( G h ). The membrane is rinsed with a streptavidin solution after which an appropriatebiotinylated, hapten specific Fab′ is added, forming complexes between the MSL α and the G h . The G h is thus tethered distant from its immobilized inner layer partners, G T , preventing the formation of dimers and lowering the electrical conductance of the membrane. The sensor is stored in this state until the addition of analyte competes with the hapten for the Fab′, liberating the channel and resulting in an increase in the membrane conductance
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How can chromoatophores be used in sensing?
Fish chromatophores from Betta splendens are used as the cytosensor element in the development of a portable microscale device capable of detecting certain environmental toxins and bacterial pathogens by monitoring changes in pigment granule distribution. Cell based biosensor prototype. The chamber containing chromatophores has wicks inserted for sample delivery,and is placed into the holder. An agent can be applied to the top wick, and the response of the chromatophores is imaged using the LED light source, lens and camera. The image is then processed by the statistical program present in the computer.A heterogeneous population of chromatophores is always present in primary tissue culture, and not all cells respond equally when exposed to many of the environmental toxins tested.
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define a chromatophore
Chromatophores, which are pigment cells responsible for the brilliant colours of fish, amphibians, and reptiles.
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considerations of function based systems (7)
1. slow (minutes to hours) response time 2. mostly lab based rather than field deployable 3. Cell based assays prone to poisoning by environmental pollutants 4. Prone to false alarms if not carefully characterised for selectivity, specificity and sensitivity 5. Process of sampling air and getting sample to the cell needs attention 6. preparation of sample adds time to the overall response time 7. needs a life support system is still living
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Examples of function based sensing
Canaries in coal mines, sarin Chickens as sentinels for encephalitis virus Daphnia to indicate water quality Poland, 8 regularly changes mussels are used to detect water quality changes
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limitations of function based sensing?
less specific less selective therefore potential for false positives is high Do not measure concentrations rely on transduction devices to measure response
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Strengths of functional detection
can detect presence of wide range of unknown chembio agents
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Describe an example hybrid system
PANTHER PAthogen Notification for THreatening Environemtnal Releases uses cellular analysis and notification of antigen risks and yields to detect pathogens including anthrax, plague, e.coli etc
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How does PANTHER work
use genetically engineered jellyfish cells to use antibodies to bind to threats. once a binding has occurred, Ca ++ are released. A bioluminescent protein from the jellyfish cells is released in response to the Ca++ increase, emitting light which is then detected.
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What do we mean by learning from nature in the context of detecting material of interest? (X5 topics)
chemical detection infrared electrical magnetic radiation
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Explain how studying nature can aid the development of situational awareness sensing systems?
radically different ways of design and manufacturing of components and systems
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What is the purpose of a conceptual model?
provides an overview of how different areas of learning from nature overlap
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Describe the 10 different types of sensing found in nature and how can they be used in a security context?
Chemical Infra red vibration pressure fluid flow strain magnetic electric fieldtouch EM radiation
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What new functionality has been derived from nature?
– Specificity and sensitivity of sensors (e.g. snake (IR), moth (chemical)) – Navigation without maps & GPS (optic flow in insects, migrating birds) – Optimised integration of sensors and processing – Sustainability, adaptive capability (physically and behaviourally) – Low processing energy demands; distributed energy.
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What Radically different way of designing and manufacturing components and systems have come from nature?
Exceptional packaging and integration Self assembly and repair Optimum low energy design (low temperature fabrication using readily available material, integration of functions, locomotion, tracking)
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6 topics in the bio design concept map
robotics bionics bioinspiration biomimetics biomimicry eco-design
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5 areas of learning from nature
Bio mimicry bio mimetics bio derived materials bio fabrication bioinspired
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define bio mimicry
Study of nature, its models, systems, processes and elements and then imitates or takes creative inspiration from them to solve human problems.
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define bio mimetics
The underlying biological paradigms present keeping each species functioning in its own unique way.
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define bio derived materials
matierals made from or originating from living organisms
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define bio fabrication
A process using cells, viruses, proteins, biomaterials and bioactive compounds as building blocks to fabricate advanced biological models, medical therapeutics and non medical biological systems
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define bio inspired
Ideas inspired by mechanisms or laws operating in biological organisms. Or a conscious strategy by designers to observe and learn principles of design from nature.
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components of a situational awareness sensing system (SASS) (*)
structure collection sampling processing (communications) surfaces / receptors power sensor element transduction
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4 components of structure for SASS
Function Scale Morphology Materials
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5 components of surfaces with relation to SASS
surface area surface modification materials connectivity degradable/fouling
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3 collection and sample types
airwater solid
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4 components of receptors in relation to SASS
antibodies bio probes synthetic ligands molecular
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3 components of processing and communications
internal collective external
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6 components of power
energy harvesting fuel cells photosynthesis/photoelectric chemical storage accumulators power management
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Chart of processing information
Digital signal processing compressive sensing biology
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What are the 4 components of DSP
collect compress reconstruct analyze/act
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3 components to CS
sparsely sample reconstruct analyse/act
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2 components to biology processing information
sparsely sample analyse/act
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fiddler crab vision example
• Biological systems compressively sample environment and do not reconstruct to make critical decisions (Compressive Sensing)• This is achieved by having hard coded, neural circuitry that responds only to necessary information (Machine learning)social zone lower levelpredator zone upper level
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human vision example of CS/ML
colour vision only in the centre, peripheral vision only in black and white
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Nature - types of chemo sensing
olfaction gustation (taste) surface contact (touch)
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What is a ROC curve?
receiver operating characteristics curve shows either how different detectors respond compared to the same parameters, or how the same detector changes its detection ability in different environments when controlled for same detection confidence and fixed response time closer to Y axis, the more real alarms are being seen closer to the x axis, the more false alarms present
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what is a detection assay?
a way of qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity
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Describe a sampling system
a system that autonomously or with human interaction through a series of agreed processes, can take a sample from an environment requiring testing, use one or more sensors to try to identify threats, then provide an response to an analyst.
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What the characteristics of a biosensor ? 14 points
specificity sensitivity speed cost reliability ease of manufacturing size weight power and consumables ability to work in complex mixtures low false positive rate multianalyte detection continuous/batch sensing ease of operation viability (live vs dead organisms)
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What is meant by the response time
time taken from a sensor reaching the limit of detection to generating an alarm
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What is LOD?
limit of detection or detection threshold - the minimum agent concentration required for a sensor to generate an alarm.
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why is LOD important?
because it is the key trigger point for a system to enter an alert state
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What is a spider chart used for?
demonstrating the results of 12 specifications of one or more sensors
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what is a spider chart?
12 spoked wheel where each spoke is a measurable requirement for the sensor system. usually the further from the centre the better the system
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12 specifications of a spider chart?
weight power consumption Unit cost reliability (MTBF) Operating costs MTBM false positive rate (low dose rate) false positive rate (high dose rate) response time detection confidence sensitivity size
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Explain deployment analysis?
assessment of 18 cardinal points to provide a final summary of how effective a system is, in ability to detect, but also maintain, continuously function and cost benefit
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units for weight
Kg though dependant on system in vivo could be mg, platform could be tonnes
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units for power consumption
watts
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unit for unit cost
£ or currency of country using device. that said, $ cost does help as an internationally understood baseline for most costings
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What is MTBF?
mean time between failures, or the average time between repairable failures of a technology product
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unit of MTBF
months, though could be context dependant to secs to years
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units for operating costs
£ per year
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What is MTBM
mean time between maintenance
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unit of MTBM
weeks, though could be system dependant
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unit of false positive rate
number per year, though could be context dependant, so covid with national screening policies could be rate per day
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response time unit
seconds
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unit of detection confidence
percentage calculated from confusion chart and ROC measurements
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unit for sensitivity
ACPLA - Agent Containing Particle per Litre of Air
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unit of size
m^3
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Is there such a thing as a one size fits all sensor?
no, and usually the opposite is true, should a sensor be described as a one size fits all, then it usually fails to meet the minimum requirements for any application.
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Define noise
is fluctuation in sensor responses due to factors that are independent of the measurement environment. a signal to noise ration of more than 1 is important, though in some cases not necessary/ possible. in these cases more processing like conducting a Fourier transform on the data is required, but this does affect the fidelity of the output.
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define clutter
is the sensor response to all factors associated with the measurement environment other than the agent.
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define signal
is the sensor’s response to the agent.
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18 cardinal points specifications for a bio sensor
specificity selectivity sensitivity speed of response stability reproducability repeatability reliability range resolution low false positive rate multi analyte detection continuous/batch sensing ease of operation viability ease of manufacture size ,weight ,power cost
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5 S's of cardinal points
specificity selectivity sensitivity speed of response stability
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4 R's of cardinal points
Range resolution repeatability reliability
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8 multi letters of cardinal points
low false positive rate multi analyte detection continuous/batch sensing ease of operating viability ease of manufacturing SWAP (size weight and power) Cost
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Summary of cardinal points (13 points)
inital cost operating cost response time limit of detection power consumption mission duration consumables maintenance reliability ruggedness/operationally hardened form factor environmental considerations