Serology (Lect 2) Flashcards
(33 cards)
T/F you use a population frequency chart for Ag and the % is frequency of presence or “incidence”
true
What is considered high incidence and low incidence?
High >90%
Low <10%
Briefly describe phenotype testing
to ID unknown Ag on RBC use Anti-Sera
Describe Abs briefly
person makes an Ab for an Ag they do NOT have on their RBCs
compatible blood is 100% population frequency of Ag
phenotype donor blood for Ag and look for negative Rxn
Whats the number one cause for transfusion rxns?
Data entry errors
Describe document control regulations
all results needed to be recordered as they are tested, even if they seem wrong
All data needs a second tech review
strict guidelines for how long documents need to be kept
all documents need to be legible and permanent
Describe sample lables
affixed lable bearing sufficient information for unique ID of Pt
2 unique ID’s
(ex date and time, collector, arm band..etc)
Describe sample integrity
EDTA preferred centrifuge to separate from plasma
NO GEL SEPARATOR
NO HEMOLYSIS
use enough volume
Describe pt Hx
ABO/rH
difficult blood typing
clinically sign Ab
adverse events to transfusion
Describe the 3-DAY RULE
samples should be retained for at least 7 days post transfusion
a sample should be obtained from pt within 3 days of scheduled transfusion
What are some reasons the 3-day rule should not be followed?
pt has been transfused in the preceding 3 mos w/ blood containing allogenic rbcs
pt has been pregnant preceding 3 mos
Hxt is uncertain
Describe quality control:
reagents
+/- controls need to be run daily
kit contents must not be mixed
manager must monitor QC once a month minimum
Describe equipment: critical equipment
directly involved in testing/storage of blood
temp needs to be monitored daily
must be maintained according to manufacturers guidlines
Describe QC daily
reagents. (ex I/A)
tests: (B/A)
before pt testing
reagents like IS/AHG
blood type/Ab screen
Describe qc Non-routine
run w/ pt testing
tests systems
reagents used for specific test
Phenotyping/ anti sera/ fetal screening
Describe BB reagents
; Ab colors
A blue
B yellow
D clear
G green (sometimes)
LONG OUTDATES
Describe BB reagents RBC ags
screen/panel
reverce cells
outdates?
3% mix for tube testing
.8% mix for gel testing
screen/panel from one human donor who is 0 type
reverse cells can be from multiple donors
OUTDATES 3-5 WEEKS
Describe handling of Ab and Ags
Ab - 2-10 deg, physical exam
Ag- 2-10deg physical exam
Describe polycolonal
two different sources directed at same Ag multiple plasma cell courses to detect mutliple sites on same Ag
Describe Monoclonal
Ab for specific Ag made from single plasma cell source to detect a single site on Ag
Describe poly-specific
Ab mixture that detects multiple Ag or detects the Ag in different ways (IgG/IgM)
Anti-D/Anti-AHG
Describe mono-specific
single Ab mixture to pick up single Ag
ABO/Ab
T/F Polyclonal is mouse and monoclonal is rabbit
false
Monoclonal is mouse
Poly is rabbit
Describe longevity
interaction based on Abs affinity and avidity
Affinity (binding)
Avidity (strength)
