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Flashcards in Serum Bilirubin Deck (16)
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Product is a red azo dye

Reaction of bilirubin with diazotised sulphanilic acid (DSA)

1

Wavelength

546 nm

2

It is directly proportional to the bilirubin concentration of the sample

Absorbance of red azo dye

3

Water soluble bilirubin glucuronides:
Albumin conjugated indirect bilirubin:

DSA
DSA in the presence of accelerator (total bilirubin = direct + indirect bilirubin)

4

Stability

Expiry date if unopened and stored at 15-18 Celsius

5

May reduce bilirubin levels

Exposure to light
Hemolysis (inhibitory effect of hemoglobin on the diazo reaction)

6

Reference range

(Review)

7

Temperature

20-25 Celsius

8

Optical path

1 cm

9

Total bilirubin

Incubate at room temperature for 10 to 30 minutes

10

Direct bilirubin

Mix, incubate at room temperature for exactly 5 minutes

11

Calculation

Bilirubin concentration [mg/dL] = absorbance at 546 nm x 13.0
[mg/dL] 17.1 = [mmol/L]

12

Sulphanilic acid + sodium nitrite --->

DSA

13

_____ ---> DIRECT Azobilirubin

Bilirubin + DSA

14

Bilirubin + DSA + accelerator --->

TOTAL Azobilirubin

15

Contents

1 Total bilirubin reagent (white cap)
2 T-Nitrite reagent (white cap)
3 Direct bilirubin reagent (red cap)
4 D-Nitrite reagent (red cap)