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Histopathology > Special Stain > Flashcards

Special Stain Flashcards

(70 cards)

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1
Q

The simplest method of differential staining of collagen

A

Van Gleson’s stain

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2
Q

The collagen color in van Gleson’s stain

A

Pink or deep red

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3
Q

Excellent and colorful method of demonstrating connective tissue fiber

A

Mallory’s aniline blue stain

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4
Q

Enumerate 4 special stain use in collagen

A

Van Gleson’s
Mason trichrome stain
Mallory aniline blue stain
Azocarmine stain

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5
Q

Enumerate the 4 elastic fiber stain

A

Weigert’s Elastic Tissue stain
Taenzer - Unna Orcein method
Krajian technique
Verhoeff stain

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6
Q

Elastic fiber appear dark blue or blue black

A

Weigert’s Elastic Tissue stain

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7
Q

Excellent stain for elastic fibers especially in dermatology

A

Taenzer- Unna Orcein Method

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8
Q

Rapid method of elasting staining

A

Krajian technique

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9
Q

Enumerate the 5 Reticulum connective tissue Fiber stain

A 
Silver impregnation technique
PAS 
Bielschowsky's method
PALFT
Gold method
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10
Q

Best stain for reticulum

A

Silver impregnation technique

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11
Q

In reticulum connective tissue fiber stains PAS stains reticulum in what color?

A

Purplish red

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12
Q

Give the special stain use in carbohydrates and connective tissue ground substance

A 
Carbohydrate
Glycogen
Acid mucopolysaccharide 
Amyloid
Basement membrain stain
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13
Q

Is a histochemical stain that will demonstrate carbohydrates with the use of periodic acid . Give also the positive color of the substance

A

PAS . Red or magenta red

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14
Q

Are the most common PAS positive substance

A

Mucoproteins

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15
Q

Oldest stain and obsolete

A

Langhann ‘s iodine

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16
Q

Give the 3 glycogen stains

A

PAS
Best carmine method
Langhann’s iodine

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17
Q

Selective and highly specific for glycogen

A

Best carmine method

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18
Q

Widely used for demonstration of glycogen

A

PAS

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19
Q

Give the 3 Acid mucopolysaccharide stain

A

Metachromic stain
Gomorri’s Aldehyde Fuchsin stain
Fluorescent Acridine Orange

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20
Q

Specific for acid mucopolysaccharide

A

Gomorri’s Aldehyde Fuchsin stain

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21
Q

Give a selective brilliant orange fluorescence

A

Fluorescent Acridine Orange Technique

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22
Q

Give the 5 amyloid stains

A 
Gram Iodine
Krajan Amyloid
Congo Red method
Metachromatic staining
Induce fluorescent staining with Thioflavine - T
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23
Q

Turn amyloid deep blue

A

Gram’s Iodine stain

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24
Q

A test for brilliant staining of amyloid. Give the positive color

A

Krajan’s amyloid stain .Red

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25
Highly selective stain for amyloid giving deep pink or red color
Congo red method
26
Stain use in amyloid and mast cell . Give the positive color
Induces Fluorescent Staining Thioflavine- T . yellow fluorescence
27
Most common method for the demonstration of basement membrane
PAS
28
Give the 5 muscle and bone stains
``` H & E stain Masson trichrome stain Van Gleson's stain Mallory's Phospotungstic Acid Hematoxylin ( PTAH ) Lissamine fast red ```
29
Routine method for bone staining
H & E stain
30
Stain muscle yellow
Van Gleson's stain
31
Show striation in skeletal and cardiac muscle fibers , Collagen . Give the positive color for Collagen
Mallory Phospotungstic Acid Hematoxylin stain
32
Collagen is yellow, muscle striation in skeletal and cardiac muscle fibers , Collagen is reddish
Lissamine Fast red
33
It is the routine staining method of choice for bone histology
Careful differentiation with Heidenhain's iron hematoxylin and more easily with eosin as a counter stain
34
4 special stains use in the Nervous system
Nissl Bodies Nerve cell bodies/Neurons Neuroglis stain Myelin sheath
35
Enumerate the 4 Nssl Bodies stain
H&E Thionine Toluidine Blue Methylene Blue
36
Nssl bodies is also known as
Tigroid aubstance
37
Nssl bodies stain that the color of the positive is Violet
Thionine
38
Enumerate the 6 nerve cell bodies
``` H&E hematoxylin and Van Gleson's and neurofibromas Mallory PTAH overnight staining in dilute giemsa Bielschowsy Technique Cajal gold sublimate method ```
39
Excellent stains for astrocytes
Mallory's PTAH
40
Stains astrocytes black and nerve cell red
Cajal Gold Sublimate method
41
The only stain use in Neuroglia stain
Silver impregnation technique
42
Enumerate 3 myelin sheath stain
Osmic Acid Technique Weigert's Pal tech Sudan black method
43
Stains myelin sheath black
Osmic acid tech
44
Myelin sheath blue black stain
Weigert's Pal technique
45
Rapid staining of myelin
Sudan black method
46
Most sensitive lipid stain
Sudan black b
47
The most commonly used stain producing a rapid permanent colorization of lipid
Sudan IV
48
Other name for Sudan IV
Scharlach R
49
Stains neutral fats and lipifuschin
Oil Red O
50
Stains fats black
OAS
51
Special stain for fats only
Nile blue sulfate
52
Excessive quantity and abnormal location of normal body pigments
Endogenous pigmentations
53
6 kinds of Endogenous pigments
``` Hemosiderine Hematoidin Hematin Hemozoin Hemofuscin Melanin ```
54
Iron containing pigments of hemoglobin
Hemosiderine
55
Hemosiderine can be stained with
Prussians blue | Turn Bulls blue
56
The iron free pigment of hemoglobin
Hematoidin
57
Hematoidin can be stained with
Gmelins test | Stein iodine test
58
Hemoglobin minus the globin molecule
Hematin
59
Black granules formed by malarial parasites
Hemozoin
60
Iron free brownish yellow pigment which occurs with Hemosiderin on hemochromatosis
Hemofuscin
61
Brown or black pigments normally found in the skin and eyes
Melanin
62
Technique use for melanin pigments
Masson Fontana technique
63
Give examples of exogenous pigments
``` Tattoo pigments Asbestos Carbon Silica Iron Silver ```
64
Enumerate the 3 artifacts pigments
Formaldehyde deposit Mercuric chloride deposit Osmium tetroxide deposits
65
Is particularly recommended for staining skeletal system in embryos and fetuses
Calcium dye lake reaction
66
2 stain used for bacteria
Acid fast stain | Gram stain
67
2stain use in spirochete
Rapid giemsa | Levaditi method
68
Fungi stain
``` Hematoxylin Gram staining Giemsa stain Ziehl Neelsen's Method Metachromasia of capsule ```
69
4 Special stains use for microorganism
Hitchkiss - McManus PAS technique Bauer feulgen technique Alicante blue method Gridley stain for fungi
70
Give the 4 special stain use in Connective tissue
Collagen Elastic fibers Reticulum connective tissue fiber stains

Histopathology (8 decks)

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