Staining

Question 1

Factor that influence the staining process that certain part of cells and tissues that are acidic in character have greater affinity for the basic dyes ,while the basic take more of the acid stains

Answer 1

Chemical

Question 2

2 factors that influence the staining process

Answer 2

Physical phenomenon of capillary osmosis

Chemical

Question 3

Is the process of applying dyed on the sections to see and study the architectural patter of the tissue and physical characteristics of the cell

Answer 3

Staining

Question 4

Process by which sections are stained with simple aqueous soln or alcoholic soln.

Answer 4

Direct staining

Question 5

Process of staining the tissue sections which requires the use of mordant

Answer 5

Indirect staining

Question 6

Used in staining to enhance the O2 binding capacity to the tissue by the process of oxidation

Answer 6

Oxidizing agent

Question 7

Rgt. Used in staining to diminish the O2 binding capacity to the tissue by the process of reduction

Answer 7

Reduction rgt.

Question 8

Example of Reducing Agent

Answer 8

Pyrogallic acid , hydroquinone

Question 9

Total removal of color previously imparted o tissues

give example

Answer 9

Bleaching , oxalic acid

Question 10

Chemical agent which heighten the color intensity crispness and selectivity of the stain.

Answer 10

Accentuator

Question 11

There is a continuous staining of the tissue sections until the desired intensity color is obtained

Answer 11

Progressive staining

Question 12

The tissue is first overstrained and then the excess stain is later removed by another rgt. Thus differentiating staining of tissue details

Answer 12

Regressive staining

Question 13

Process of applying less or exposing tissue to dyes for shorter period of time

Answer 13

Understaining

Question 14

Process of applying dye in excess or of exposing the tissue to dye in longer period of time

Answer 14

Overstaining

Question 15

The application of a diff color stain to provide contrast and back ground to the stain of the structural components to be demonstrated

Answer 15

Counter staining

Question 16

The ability of the dye to produce one color only

Answer 16

Monochromasia

Question 17

The ability of the dye to produce different color to the tissue

Answer 17

Polychromasia

Question 18

Process in which a dye forms other dyes spontaneously by virtue of chemical rxn. Which take place such as in the oxidation of methylene blue in soln upon standing resulting of 2 dyes homologous Azure A and B

Answer 18

Polychroming

Question 19

The ability of the dye to produce different colors of different tinge or hues to the elements of the tissue

Answer 19

Metachroming

Question 20

Used to demo star the general relationship of tissue and cells with general differentiation of nucleus and cytoplasm w/o necessarily emphasizing the inclusion bodies

Answer 20

Micro anatomical staining

Question 21

Process of staining the living body

Answer 21

Vital staining

Question 22

Demonstrate minute specific structure in cytoplasm and nucleus without necessarily differentiating tissue in general

Answer 22

Cytoplasmic staining

Question 23

Used to demonstrate bacterial morphology . The substance and the organism are mixed on the slide and examined microscopically,the unstained organism a black background

Answer 23

Negative staining

Question 24

Process of coating slides with dilute celloiden soln. For greater adherence of sections to slide so they will not detached off during stain

Answer 24

Collodioniation

Question 25

Process of injecting the dye or stain to the living body

Answer 25

Intravital staining

Question 26

Process of staining the tissue sections with a little differentiation except between the nucleus and cytoplasm

Answer 26

Routine staining

Question 27

Group of benzene ring which can impart net change to the molecule by conferring the properly of electrolyte dissociation

Answer 27

Auxochrome

Question 28

Substance that passes chromophores grp of benzene ring which confers color and alter light resonance properties

Answer 28

Chromagen

Question 29

Demonstrate minute specific structure found in the cytoplasm and nucleus without necessarily differentiate tissue structural in general

Answer 29

Substantive

Question 30

Physical phenomenon of capillary osmosis

Answer 30

S.A.A

Question 31

Dyes are divided in 3 Categories

Answer 31

S.I.N ( Natural Dyes , InOrganic Dye , Synthethic Dye)

Question 32

4 types of Natural Dyes

Answer 32

Hematoxylin , Orcein , Carmine , Saffron

Question 33

Purpose of Staining

Answer 33

To enable to study and see the physical characteristic of tissue as well as their cell constituent. To identify the different tissue components through there color reaction To increase the optical density of tissue for better microscopic study

Question 34

3 types of Iron Hematoxylin

Answer 34

Weigert , Haidenhain Hematoxylin , Phospotungstic acid Hematoxylin

Question 35

Type of Iron hematoxylin use for demonstrating muscle fiber and connective tissue

Answer 35

Weigert's

Question 36

Type of Iron hematoxylin recommended for regressive staining

Answer 36

Haidenhain hematoxylin

Question 37

Type of Iron hematoxylin usually use in progressive

Answer 37

Phosphotungstic Acid Hematoxylin

Question 38

4 types of Hematoxylin

Answer 38

Mayers Hematoxylin , Ehrlich Hematoxylin , Coles Hematoxylin , Harris Hematoxylin

Question 39

Type of Hematoxylin generally used for regressive staining differentiated with 1 % HCL in 70 % acid alcohol until nucleus is selectively stained

Answer 39

Ehrlich

Question 40

Type of Hematoxylin chemically ripened with sodium ioadate . It can be used as regressive stain but it is also useful as a progressive stain

Answer 40

Mayer

Question 41

Type of Hematoxylin recommended for routine purporse , especially used in sequence with Celestine Blue

Answer 41

Coles's

Question 42

Type of Hematoxylin that is a good regressive stain that may either be used immediately or stored for future use

Answer 42

Harris

Question 43

Are substance with definite atomic groupings and are capable of producing visible colors

Answer 43

Chromophore

Question 44

An auxiliary radical or substance which imparts to the cpd the property of electrolytic dissociation , thereby altering the shade of the dye , enabling it to form salts with another cpd and ultimately retaining its color

Answer 44

Auxochrome

Question 45

Hematoxylin is a natural dye that comes from the extract ether of what tree

Answer 45

H. Campechainum

Question 46

Cochinial name of the Bug

Answer 46

Coccus cacti

Question 47

Saffron name is natural pigment extracted by the stomata of

Answer 47

Croccussativous