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MLT Microbiology > Specimen Processing > Flashcards

Specimen Processing Flashcards

(45 cards)

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1
Q

Media for Throat and Nasopharyngeal (6 and under)

A

BAP, 35 C aerobic
CHOC, 35 C in CO2

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2
Q

Normal Flora for throat and nasopharyngeal (6 and under)

A
  • alpha and gamma strep
  • Commensalism Neisseria
  • S. Epidermiditis diptheroids
  • S. Pneumoniae Anaerobes
  • N Meningitidis
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3
Q

Common pathogens in throat

A
  • Group A- beta hemolytic strep
  • H. Influenza
  • C. Diphtheria
  • B. Pertussis
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4
Q

Common pathogens in nasopharyngeal (6 and under)

A

Group A-beta hemolytic strep
N. Meningitides
S. Pneumoniae
H. Influenza

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5
Q

What do all negative strep screens need?

A

Throat culture

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6
Q

Media for Respiratory

A
  • CHOC, 35C n CO2
  • MacConkey, aerobic
  • Anaerobic media
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7
Q

Normal Flora for Respiratory

A
  • Alpha and gamma strep
  • commensal Neisseria
  • S. Epidermidis diptheroids
  • Haemophilus
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8
Q

Common pathogens of respiratory

A
  • Group A - beta hemolytic strep
  • H. Influenza
  • S. Aureus
  • Pseudomonas species
  • S. Pneumoniae
  • Enterobacteriaceae
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9
Q

Describe the loop used for urine cultures

A

Platinum loop: 0.001 or 0.01

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10
Q

Media for Urine (clean catch)

A

MacConkeys, 35C aerobic

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11
Q

Normal flora for urine

A

None

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12
Q

Common pathogens of urine

A
  • E. Coli and other enterics
  • Enterococci
  • Pseudomonas Species
  • Staphylococci aureus
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13
Q

Media for stool

A
  • BAP
  • MacConkeys
  • EMB
  • XLD
  • HE
  • SS
  • Selenite F, 35 C aerobic
  • Campylobacter plate or Skirrows in 45C, 5% in O2
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14
Q

Media for bloody stool

A

Mac with sorbitol

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15
Q

Normal flora for stool

A
  • Anaerobes
  • enterobacteriaceae
  • Enterococci
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16
Q

Common pathogens in stool

A
  • Salmonella species
  • Shigella species
  • E. Coli
  • Clostridium difficile ( if bloody and patient on antibiotics)
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17
Q

What should be commented on in stool culture?

A

Staph or yeast

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18
Q

Media for genital

A

Modified Thayer-Martin, 35C in CO2

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19
Q

Normal flora for genital

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A
  • Lactobacilli
  • Corynebacterium
  • CN Staph species
20
Q

Common pathogens for genital

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A

N. Gonorrhoeae

21
Q

What stain is used for genital?

22
Q

What is considered sterile fluids?

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A
  • CSF
  • Pleura
  • Joint
  • peritoneal
  • etc..
23
Q

Media for sterile fluids

Study These Flashcards
A
  • blood agar
  • CHOC agar, 35C in CO2
  • Thioglycolate
  • Send out anaerobes in chopped meat or thio
  • for peritoneal, set up Mac
24
Q

Normal Flora for sterile fluids

25
Common pathogens of sterile fluids
Any isolate potentially significant
26
Stain used for sterile fluids
- gram stain - CSF = India ink and hold 72-96 hours)
27
Media for blood
- submitted in 2 bottles: one 35C aerobic and one 35C anaerobic
28
Media for positive blood culture
- plate to: BAP, 35 C - CHOC agar, 35C in CO2
29
Normal flora for blood
None
30
Common pathogens in blood
None
31
Common pathogens in positive blood culture
Any isolate potentially significant
32
What stain is used for positive blood cultures?
Gram stain
33
Media for vaginal
- BAP - CHOC, 35C, CO2 - MacConkeys - thioglycolate - potato dextrose agar - Columbia Colistin - Nalidixic acid agar (CAN) - Vaginitis agar - Modified Thayer-Martin 35C, CO2
34
Normal flora for vaginal
- Lactobacilli - Acinetobacter - Corynebacterium - CN staph - Doderlein bacilli
35
Describe Doderlein Bacilli
Mixture containing yeasts, corynebacteria, and mycoplasmas
36
Common pathogens in vaginal
- N. gonorrhoeae - Gardenerella vaginalis - Candida albicans - S. Aureus ( if predominant) - Bacteroides species (anaerobe - if predominant) -
37
What stain is used for vaginal
Gram stain
38
Describe listeria of vaginal
Listeria looks like beta-strep but is catalase positive
39
Media for superficial wounds (ears and eyes included)
- BAP - CHOC 35C in CO2 - MAC - PEA - 2 anaerobic plates 35C - anaerobic
40
Normal flora in superficial wound
- bacillus species - S. Epidermidis diptheroids - S. Aureus - Micrococci - Peptococcus - Propionebacterium Acne’s - Candida albicans - Commensal Neisseria - Mycobacterium - Clostridium - Erysipelothrix rh - many more anaerobes
41
Common pathogens of superficial wound
- S. Aureus - Beta-hemolytic strep - P. Aeruginosa - H. Aegypticus (eye) - H. Influenza (biotype III) - Enterobacteriaceae
42
Stain used for wounds
Gram stain
43
Media for deep wound
- same as superficial wound except thioglycolate broth or schadlers Broth
44
Normal flora for deep wounds
None
45
Common pathogens of deep wounds
- S. Aureus - Beta-hemolytic strep - P. Aeruginosa - H. Aegypticus (eye) - H. Influenza (biotype III) - Enterobacteriaceae - anaerobes - isolates - identify any growth

MLT Microbiology (9 decks)

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