SPECTROPHOTOMETRY PART 1

Question 1

• monochromator used is either a prism or a diffraction gratig

Answer 1

spectro

Question 2

• wavelength selector

Answer 2

monochromator

Question 3

• filter

Answer 3

photometer

Question 4

Electrons are excited when subjected to the heat of a flame. as they return to a ground state, they emit radiation of a characteristic wavelength

Answer 4

FLAME PHOTOMETRY

Question 5

FLAME PHOTOMETRY

Component parts

Answer 5

Atomizer, flame air and gas supply, monochromator, detector, readout device

Question 6

FLAME PHOTOMETRY

Use

Answer 6

Na+, K+, Li+

Question 7

Sodium =

Answer 7

yellow

Question 8

Rubidium =

Answer 8

red

Question 9

Potassium =

Answer 9

violet

Question 10

Magnesium =

Answer 10

blue

Question 11

Lithium =

Answer 11

red

Question 12

mixture of Hydrogen and Oxygen gas

Answer 12

Gases for Flame Photometry

Question 13

Oxygen gas

Answer 13

Natural gas
Acetylene
Propane

Question 14

• for Na and K determination

Answer 14

Propane

Question 15

Burner Assembly

Answer 15

aspirator
atomizer/nebulizer
flame

Question 16

breaks the chemical bonds to produce atoms

Answer 16

Burner Assembly

Question 17

cause excitation of the electrons of an atom

Answer 17

Burner Assembly

Question 18

Internal standard:

Answer 18

Lithium or Cesium

Question 19

Burner Assembly Advantages:

[?] intensity
absent from [?]
emits wavelength different from [?]
(?) as radiation buffer

Answer 19

high emision
biological fluid/ trace element
Na and K
Li

Question 20

Burner Assembly Importance:

Answer 20

to achieve stability

Question 21

TWO TYPES OF BURNERS OF A FLAME PHOTOTMETER

Answer 21

TOTAL CONSUMPTION BURNER
LAMINAR FLOW BURNER OR PRE-MIX BURNER

Question 22

Gases are passed at high velocity over the end of a capillary suspended in the solution, causing liquid to be drawn up through the capillary into the flame

Answer 22

TOTAL CONSUMPTION BURNER

Question 23

Gravitational feeding of solution through a restricting capillary into an area of high velocity gas flow where small droplets are produced and passed into the flame

Answer 23

LAMINAR FLOW BURNER OR PRE-MIX BURNER

Question 24

ESSENTIAL PARTS OF THE FLAME PHOTOMETER

Answer 24

MONOCHROMATOR

Question 25

Interference filter

Answer 25

MONOCHROMATOR

Question 26

Maximum amount of the line emission to pass through the detector

Answer 26

narrowest band path

Question 27

: giving-off of various wavelengths by non-ionic materials

Answer 27

Continuous emission

Question 28

Measures light absorbed by ground state atoms.

Answer 28

ATOMIC ABSORPTION SPECTROPHOTOMETRY

Question 29

ATOMIC ABSORPTION SPECTROPHOTOMETRY Band path width:

Answer 29

0.001 to 0.01 nm

Question 30

ATOMIC ABSORPTION SPECTROPHOTOMETRY Component parts

Answer 30

Hollow cathode lamp (with argon or neon gas), atomizer, burner, monochromator, detector, readout device

Question 31

ATOMIC ABSORPTION SPECTROPHOTOMETRY Use

Answer 31

Calcium Magnesium Copper Zinc Lead