Staining

Question 1

Process of applying dyes on the section to see and study
the architectural pattern of the tissue and physical
characteristics of cells

Answer 1

staining

Question 2

Tissue components are demonstrated by direct
interaction with a dye or staining solution

Answer 2

Histological stain

Question 3

Active tissue components are colored

Answer 3

histological

Question 4

Tissue components are studied through chemical
reactions

stains fats, carbiohydrates

Answer 4

Histochemical/Histochemistry

Question 5

Instead of stains/dyes, uses antibodies

Combination of histochemical and immunologic
technique (immunologic because instead of dyes,
used antibodies)

Answer 5

Immunochemical

Question 6

uses aqueous or alcoholic dye solutions to
produce a color;

Answer 6

Direct staining

Question 7

uses a dye with the help of mordant or accentuator

Answer 7

Indirect staining

Question 8

substance that serves as link or bridge
between the dye and the tissue

Answer 8

mordant

Question 9

Potassium aluminum

Answer 9

Alum hematoxylin

Question 10

Mayer’s Hematoxylin — incorporated
mordant
Coles
Harris
Gill’s
Erhlich’s
Delafield’s

Answer 10

Mordant

Question 11

ferric ammonium chloride

Answer 11

Weigert’s hematoxylin

Question 12

ferric ammonium sulfate

Answer 12

Heidenhain’s Hematoxylin

Question 13

• substance that heightens the colors
  intensity of the dye and the selectivity of the dye;
  increases the staining power of the dye

Answer 13

accentuator

Question 14

Tissue:

1st overstained
Excess dye removed through decolorization

Answer 14

Regressive Staining

Question 15

refers to the
selective removal of excess dye

Answer 15

DECOLORIZATION/DIFFERENTIATION

Question 16

staining a tissue with a color that is different
from the stain itself; uses a dye that will produce
a different color from the dye used

Answer 16

Metachromatic Staining

Question 17

USED TO STAIN EPITHELIAL MUCINS, MAST
CELL GRANULES, CARTILAGE, AMYLOID

Answer 17

Metachromatic staining

Question 18

Bismarck brown
Safranin
Azure A
Azure B
Azure C

Answer 18

Metachromatic stain

Question 19

staining technique in
which cells and tissues are stained with a same
shade/hue as that of the dye

Answer 19

orthochromatic staining

Question 20

involves application of a different color/another
dye to produce contrast in background

Answer 20

counter staining

Question 21

red acid dye; cytoplasmic stain; COMMONLY USED

Answer 21

Eosin

Question 22

Tissue elements are demonstrated NOT by stains
but by colorless solutions of metallic salts

Answer 22

Metallic impregnation

Question 23

Metallic salts Leaves black deposit on tissue
surface

Answer 23

Not dyes

Question 24

used to demonstrate living cells (viable
cells/fresh)

Answer 24

Vital staining

Question 25

dye is injected to any part of the animal body

Answer 25

Intravital

Question 26

stain is applied immediately to tissue after its removal from the living body

Answer 26

supravital

Question 27

Supra vital stains (NJTNTT)

Answer 27

Neutral red — best vital stain Janus green — used for mitochondria Trypan blue Nile blue Thionine – metachromatic staining; supravital staining Toluidine blue – metachromatic staining; supravital staining

Question 28

H & E: NUCLEUS COLOR

Answer 28

BLUE TO BLUE BLACK

Question 29

H & E: KARYOSOME

Answer 29

DARK BLUE

Question 30

H & E: CYTOPLASM

Answer 30

PALE PINK

Question 31

H & E: RBC, EOSINOPHILIC GRANULES, KERATIN

Answer 31

BRIGHT ORANGE

Question 32

H & E: CALCIUM AND DECALCIFIED BONE

Answer 32

PURPLISH BLUE

Question 33

H & E: DECALCIFIED BONE MATRIX, COLLAGEN, OSTEOID

Answer 33

PINK

Question 34

H & E: MUSCLE FIBER

Answer 34

deep pink

Question 35

Staining method for frozen section

Answer 35

H and E Thionine Polychrome methylene blue alcoholic pinacynol method

Question 36

Hematoxylin, Chochineal dyes, Orcein, Saffron derived from plants and animals

Answer 36

natural dyes

Question 37

active coloring agent of hematoxylin

Answer 37

hematin

Question 38

RIPENING AGENTS: Sodium Iodate (3)

Answer 38

Erlich's hematoxylin Mayer's hematoxylin - with citric acid; chloral hydrate Gill's - mucin and goblet cell

Question 39

RIPENING AGENTS: Mercuric oxide exfoliative cytology and demonstrating sex chromosomes

Answer 39

Harris hematoxylin

Question 40

RIPENING AGENTS: alcoholic iodine

Answer 40

Cole's hematoxylin

Question 41

RIPENING AGENTS: Glycerol

Answer 41

Delafield's - wine smell; purple red shade

Question 42

RIPENING AGENTS: Potassium iodate

Answer 42

Carazzi's

Question 43

Iron hematoxylin mordant: Ferric ammonium chloride

Answer 43

Weigert's hematoxylin - muscle fibers and ct

Question 44

Iron hematoxylin mordant: Ferric ammonium sulfate

Answer 44

Heidenhain's hematoxylin - nuclei and cytoplasmic inclusion

Question 45

Demonstrate spermatogenesis

Answer 45

Copper hematoxylin

Question 46

phosphotungstic acid: hematoxylin

Answer 46

Tungsten hematoxylin

Question 47

demo of granules of endocrine cells

Answer 47

Lead hematoxylin

Question 48

demo of collagen, reticulin and argentaffin cells

Answer 48

Molybdenum hematoxylin

Question 49

extracted from bug treated with alum to produce CARMINE

Answer 49

conchineal dyes

Question 50

picric acid with carmine: neuropatholocigal studies

Answer 50

picrocarmine

Question 51

best carmine for glycogen

Answer 51

aluminum chloride

Question 52

vegetable dye extracted from lichens, used for staining ELASTIC FIBERS

Answer 52

orcein

Question 53

vegetable dye extracted from lichens Not used as stain, used for indicator Poor staining property

Answer 53

Lithmus

Question 54

responsible for the coloring property/imparting color

Answer 54

chromophore

Question 55

responsible for the dyeing property; property of retaining the imparted color

Answer 55

auxochrome

Question 56

DO NOT have auxochrome but they have chromophore

Answer 56

lysochrome or oil soluble dyes

Question 57

Fat stains: most sensitive

Answer 57

Sudan Black B

Question 58

for neutral lipids: stains red

Answer 58

Sudan IV

Question 59

fat stain for CNS tissues: first sudan dye to histochemistry

Answer 59

Sudan III