STAINING LAB (2)

Question 1

process of applying dyes on the sections to see and study the
architectural pattern of the tissue and physical characteristics of the
cells

Answer 1

Staining

Question 2

tissues and cells display varying affinities for most dyes and stains used during the process

Answer 2

Staining

Question 3

Has greater affinity for basic dyes

Answer 3

Acidic Structure

Question 4

Has greater affinity for acidic dyes

Answer 4

Basic structures

Question 5

a related procedure that makes use of heavy metal salts that are selectively precipitated on certain cellular and tissue components

Answer 5

Impregnation

Question 6

used for silver staining of nervous tissue and demonstration of reticulin

Answer 6

Impregnation

Question 7

What is the most commonly used reagent in impregnation?

Answer 7

Silver nitrate

Question 8

What are the three major types of Staining?

Answer 8

1. HISTOLOGICAL STAINING
2. HISTOCHEMICAL STAINING/HISTOCHEMISTRY
3. IMMUNOHISTOCHEMICAL STAINING

Question 9

tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution

Answer 9

HISTOLOGICAL STAINING

Question 10

producing coloration of the active tissue component

Answer 10

HISTOLOGICAL STAINING

Question 11

employed to demonstrate the general relationship of tissues and cells with differentiation of nucleus and cytoplasm

Answer 11

HISTOLOGICAL STAINING

Question 12

Examples: microanatomic stains, bacterial stains and specific tissue stains
(muscles, CT, and neurologic stains)

Answer 12

HISTOLOGICAL STAINING

Question 13

tissue constituents are studied through chemical reactions that will permit microscopic localization of a specific tissue substance

Answer 13

HISTOCHEMICAL STAINING/HISTOCHEMISTRY

Question 14

Examples: Perl’s Prussian blue reaction for hemoglobin, Periodic Acid Schiff staining for carbohydrates

Answer 14

HISTOCHEMICAL STAINING/HISTOCHEMISTRY

Question 15

active reagent serves as the substrate upon which the enzymes act

Answer 15

Enzyme histochemistry

Question 16

final opacity of coloration produced from the substrate rather than the tissue

Answer 16

Enzyme histochemistry

Question 17

combination of immunologic and histochemical techniques that allow phenotypic markers to be detected and demonstrated

Answer 17

IMMUNOHISTOCHEMICAL STAINING

Question 18

what are the labels that immunohistochemical staining is using

Answer 18

monoclonal/polyclonal, fluorescent-labeled, enzyme-labeled antibodies

Question 19

What are the methods of staining?

Answer 19

DIRECT STAINING
INDIRECT STAINING
PROGRESSIVE STAINING
REGRESSIVE STAINING
METACHROMATIC STAINING
COUNTERSTAINING
METALLIC IMPREGNATION
VITAL STAINING

Question 20

process of giving color to the sections by using aqueous or alcoholic dyes

Answer 20

DIRECT STAINING

Question 21

process whereby action of dye is intensified by adding another reagent which serves as a link/bridge between tissue and dye making staining reaction possible

Answer 21

INDIRECT STAINING

Question 22

serves as a link/bridge between tissue and dye making staining reaction possible

Answer 22

(MORDANT)

Question 23

may be applied to tissue before staining or may be included in the staining process, or may be incorporated as part of the dye solution itself

Answer 23

Mordant

Question 24

not essential to the chemical union of tissue and dye; does not participate in the staining reaction, but merely accelerates or hastens the speed of staining reaction by increasing the staining power and selectivity of the dye

Answer 24

ACCENTUATOR

Question 25

process whereby tissue elements are stained in a definite sequence, and the staining solution is applied for specific periods of time or until the desired intensity of coloring of the different tissue elements is attained

Answer 25

PROGRESSIVE STAINING

Question 26

no decolorization or washing after the application of the dye

Answer 26

PROGRESSIVE STAINING

Question 27

tissue is first over stained to obliterate cellular details, and excess stain is removed or decolorized from unwanted parts of the tissue, until the desired intensity of color is obtained

Answer 27

REGRESSIVE STAINING

Question 28

selective removal of excess stain from the tissue so that a specific substance may be stained distinctly from surrounding tissues

Answer 28

DIFFERENTIATION/DECOLORIZATION

Question 29

use of dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself (metachromasia)

Answer 29

METACHROMATIC STAINING

Question 30

basic dyes belonging to the thiazine and triphenylmethane groups

Answer 30

METACHROMATIC STAINING

Question 31

stain tissues in color shades that are similar to the color of the dye itself

Answer 31

ORTHOCHROMATIC STAINING

Question 32

application of a different color or stain to provide contrast and background to the staining of structural components to be demonstrated

Answer 32

COUNTERSTAINING

Question 33

In the cytoplasmic stains, what is the color of eosin Y, Eosin B, Phloxine B?

Answer 33

Red

Question 34

In the cytoplasmic stains, what is the color of piciric acid, orange G, Rose Bengal?

Answer 34

Yellow

Question 35

In the cytoplasmic stains, what is the color of light green SF, Lissamine green?

Answer 35

Green

Question 36

Under nuclear stains, what is the color of neutral red, safranin 0, carmine, hematoxylin?

Answer 36

Red

Question 37

Under nuclear stains, what is the color of methylene blue, toluidine blue, Celestine blue?

Answer 37

Blue

Question 38

process where specific tissue elements are demonstrated, not by stains, but by colorless solutions of metallic salts which are thereby reduced by the tissue, producing an opaque, usually black deposit on the surface of the tissue

Answer 38

METALLIC IMPREGNATION

Question 39

agent is not absorbed by the tissue, but is held physically on the surface as a precipitate or as a reduction product

Answer 39

METALLIC IMPREGNATION

Question 40

selective staining of living cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle

Answer 40

VITAL STAINING

Question 41

done by injecting dye into any part of the body (intravenous, intraperitoneal or subcutaneous), producing specific color

Answer 41

INTRAVITAL STAINING

Question 42

stain living cells immediately after removal from the living body

Answer 42

SUPRAVITAL STAINING