Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histopath Lec > Staining Part 1 > Flashcards

Staining Part 1 Flashcards

1
Q

Cause of Tissue block smells like xylene

A

Incomplete impregnation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Cause of Moist block that tends to crumble

A

Incomplete impregnation, problem in embedding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Cause of Air holes on trimmed tissue block

A

Incomplete impregnation, problem in embedding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Cause of Very hard tissues; tissue shrinkage

A

Over fixation, over dehydration, over clearing, overheated paraffin wax

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Cause of Tissue becomes opaque

A

Problem in clearing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Cause of Soft and mushy tissue

A

Under fixation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Cause of Clearing agent becomes milky

A

Incomplete dehydration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Cause of Brittle; hard tissue

A

Over fixation, over dehydration, over clearing, overheated paraffin wax

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Application of dyes on tissue sections to study the architectural patterns and physical characteristics of cell

A

Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What stain is used for nucleus?

A

basic stains (basophilic)- Acidic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What stain is used for cytoplasm?

A

acidic stains (acidophilic)- Basic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Groups of tissue staining: (3)

A

Histologic Stains
Histochemical/Histochemistry
Immunohistochemical Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

produce coloration of the active tissue component

A

Histologic Stains

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

tissue constituents are demonstrated in section by direct interaction with a dye

A

Histologic Stains

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

tissue is demonstrated thru chemical reaction

A

Histochemical/Histochemistry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

active agent of stain is used as substrate upon which enzyme acts

A

Enzyme histochemistry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Stains under histochemical/histochemistry (2)

A

Perl’s Prussian Blue
Periodic Acid Schiff

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Stain for hemoglobin and its color

A

Perl’s Prussian Blue
Blue color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Stain for carbohydrates and its color

A

Periodic Acid Schiff
Red color or orange

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

detects tissue antigen

A

Immunohistochemical Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

detection of phenotypic markers that are detected by antibodies

A

Immunohistochemical Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Example of Immunohistochemical Staining: (2)

A

Monoclonal, Polyclonal labeled antibodies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Methods of staining (8)

A

Direct staining
Indirect staining
Progressive staining
Regressive staining
Metachromic staining
Counterstaining
Metallic Impregnation
Vital staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

uses aqueous or alcoholic dyes to give color ex. Methylene blue, eosin

A

Direct staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

the action of the dye is intensified by addition of another agent (mordant & accentuator)

A

Indirect staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

serves as a link or bridge between the tissue and the dye

A

Mordant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

no decolorizer; staining with specific periods of time or until desired color is attained (gradual application)

A

Progressive staining

28
Q

tissue is overstained first, removed excess stain by decolorization

A

Regressive staining

29
Q
  • basic dyes or cations
  • staining tissue with a color that is different from the stain itself (can be used to stain
    Connective tissue, epithelial mucins amyloid, mast cell granules)
A

Metachromic staining

30
Q

Metachromic staining can be used to stain (4)

A

Connective tissue
epithelial mucins
amyloid
mast cell granules

31
Q

Example of metachromic stains (9)

A

methyl violet/ Crystal violet, Cresyl blue, Safranin, Bismarck brown, Basic Fuchsin, Methylene blue, Thionine, Toluidine blue and Azure A,B,C

32
Q

involves application of different color to produce contrast and background

A

Counterstaining

33
Q

CYTOPLASMIC STAINS: red

A

Eosin Y
Eosin B
Phloxine B

34
Q

CYTOPLASMIC STAINS: Yellow

A

Picric acid
Orange G
Rose Bengal

35
Q

CYTOPLASMIC STAINS: greem

A

Light Green SF
Lissamine green

36
Q

Cytoplasmic stains: (3)

A

Red
Yellow
Green

37
Q

Nuclear stains (2)

A

Red
Blue

38
Q

NUCLEAR STAINS: red

A

Neutral red
Safranin O
Carmine
Hematoxylin

39
Q

NUCLEAR STAINS: blue

A

Methylene blue
Toluidine blue
Celestine blue

40
Q

process where specific tissue elements are demonstrated, not by stains, but by colorless solutions of metallic salts

A

Metallic Impregnation

41
Q

-reduced by the tissue, producing an opaque, usually black deposit on the surface of the tissue or bacteria

A

Metallic Impregnation

42
Q

Example of Metallic Impregnation (2)

A

Gold chloride
Silver nitrate

43
Q

selective staining of living cells constituents

A

Vital staining

44
Q

______ is resistant and therefore not demonstrated (if stained it is indicative of cell
death)

A

Nucleus

45
Q

Types of vital staining (2)

A

Intravital stain
Supravital stain

46
Q

stain is injected to any part of the living body (Intravenous, Intraperitoneal, Subcutaneous)
Ex: Lithium, Carmine, Indian Ink

A

Intravital stain

47
Q

the stains applied immediately after removal of cells from the living body

A

Supravital stain

48
Q

best vital stain

A

Neutral Red

49
Q

Vital stain for mitochondria

A

Janus Green

50
Q

most common method for microanatomical studies of tissues

A

Routine H and E staining

51
Q

Initial Xylene Bath:

A

for further Decolorization

52
Q

Descending grades of Alcohol

A

for Hydration

53
Q

Application of Hematoxylin:

A

Nuclear stain (primary dye)

54
Q

Application of Alcohol:

A

for Differentiation/Decolorization

55
Q

Application of Ammonia water:

A

blueing agent to intensify the color of the nucleus

56
Q

Application of Eosin:

A

Cytoplasmic stain (Counterstain)

57
Q

Ascending Grades of Alcohol:

A

for Dehydration

58
Q

Last Xylene Bath:

A

clearing prior to mounting

59
Q

Color results of Nuclei

A

Blue to Blue black

60
Q

Color results of Karyosome

A

Dark blue

61
Q

Color results of Cytoplasm

A

Pale pink

62
Q

Color results of Muscle fibers

A

Deep pink

63
Q

Color results of Calcium and Decalcified bones

A

Purplish blue

64
Q

Color results of RBCs, Eosinophilic granules, Keratin:

A

Bright orange

65
Q

Color results of Decalcified bone matrix, collagen and osteoid:

A

pink

66
Q

accelerates or hastens the staining process

A

Accentuator

Histopath Lec (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions