Staining Procedures Flashcards
-commonly used dyes in bacteriology
-cationic/+ charge binds to negatively charged molecules
basic dyes
-anionic dyes that binds to positively charged cells
-EOSIN, ROSE BENGAL, ACID FUSCHIN
acidic dyes
staining technique:
directed towards coloring the forms and shapes
simple staining
staining technique:
-divided bacteria into groups
-directed towards coloring components of elements
differential staining
type of staining technique:
-GRAM STAINING
-AFB
differential staining
staining technique:
directed at identification of organism
diagnostic antibody or DNA probe-mediated staining
staining technique:
-demonstrate presence of diffuse capsule surrounding bacteria
-study gas vacuoles and viral morphology
-study cells sensitive to heat fixing
-appears light against dark background
negative staining
type of staining technique:
INDIA INK, NIGROSIN DYE
negative staining
most used differential stain in lab
gram stain
theory
-Gram +: positive
-Gram -: negative
Mg RNA
theory
-Gram +: less permeable to decolorizing agent
-Gram -: permeable to decolorizing agent
benian
theory
-Gram +: low isoelectric point
-Gram -: high isoelectric point
stearn and stearn/ magnetism
gram stain
purple/violet
gram +
gram stain
pink
gram -
used to stain bacteria that have high lipid content in cell walls
acid fast stain
acid fast stain
red
acid-fast bacilli
acid fast stain
blue-green
non-acid fast bacilli
acid fast staining methods:
hot staining method
ziehl neelsen
acid fast staining methods:
cold staining method
kinyouns
acid fast staining methods:
differentiates M. smegmatis from M. tuberculosis
baumgartens
acid fast staining methods:
selective for cell wall of AFB
auramine-rhodamine
3 grams carbolfuchsin + 5% phenol
ziehl neelsen
4 grams carbolfuchsin + 9% phenol
kinyouns
ideal for concentrated smears and partially AFB like nocardia
ziehl neelsen
