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Sthapylococcus Flashcards

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1
Q

Sthapylococcus Grow in media containing

A

10% NaCl at temp 18 to 40 C

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2
Q

Staphylococcus Present on the

A

skin and mucus membrane

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3
Q

Staphylococcus Resistant to

A

drying and heat

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4
Q

Slime layer or capsule

What kind of layer

A

Polysaccharide

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5
Q

Slime layer or capsule

Protects bacteria

A

Inhibition of chemotaxis and phagocytosis

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6
Q

Slime layer or capsule

Facilitate adherence to

A

Catheters and synthetic materials

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7
Q

Slime layer or capsule

Covalently linked to

A

Peptidoglycans

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8
Q

Slime layer or capsule

Has affinity to

A

FC receptor of IG

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9
Q

Slime layer or capsule

Blocks

A

Opsonization and phagocytosis

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10
Q

Ribitol teichoic acid with N-acetylglucosamine (Polysaccharide A)

A

S. aureus

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11
Q

Glycerol teichoic acid with glucosyl residues (polysaccharide B)-

A

S. epidermidis

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12
Q

Disrupts smooth muscle in the blood vessel
Toxic to RBC, platelets, hepatocytes
Cause cell lysis-efflux of K, influx Na, Ca- osmosis
Septic shock

A

Alpha

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13
Q

Damage membrane of susceptible cells
Lyze neutrophils and macrophage
Cell lysis
Necrotizing skin infection

A

Gamma

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14
Q

Disrupts cell membrane

Toxic to variety of cells

A

Delta

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15
Q

Specific for sphingomyelin and lysophosphatidylcholine
Toxic to RBC, WBC, macrophage ,fibroblast
Tissue destruction and abscess formation

A

Beta

Sphingomyelinase C

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16
Q

Kills WBC, leucotoxic

A

Phanton valentine

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17
Q

Exfoliative toxin

Heat stable

A

ETA

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18
Q

Exfoliative toxin

Heat Labile

A

ETB

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19
Q

Exposure of exfoliative toxin

A

splitting of desmosomes or intercellular bridges in the stratum granulosum epidermis

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20
Q

Exfoliative toxin common in neonates. ETA and EBA binds to

A

GM4 like glycolipids

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21
Q

Entertoxin

A

A-E

G-I

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22
Q

Entertoxin are

A

Stable to heating

Resistant to hydrolysis

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23
Q

o – most commonly associated with disease

A

Enterotoxin A

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24
Q
  • contaminated milk products
A

oEnterotoxin C and D

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25
- Pseudomembranous colitis
oEnterotoxin B
26
TSST -1 Formerly pyrogenic
Exotoxin C | Entertoxin F
27
TSST -1 Induce cytokine release from
Macrophage and T cells
28
TSST -1 Increase sensitivity to
Endotoxin
29
TSST -1 Produce leakage of
Endothelial cells
30
TSST -1 Penetrate
Mucosal barrier
31
Enzymes Fee and bound
Coagulase
32
Enzymes Coagulate convert fibrinogen to
Insoluble fibrin to staphylothrombin
33
Enzymes Cause formation of fibrin around abscess protecting organism from phagocytosis
Clumping factor
34
Enzymes Catalyze the conversion of toxic hydrogen peroxide to water and oxygen
Catalase
35
hydrolyzes hyaluronic acid in acellular matrix of connective tissue to spread
Hyaluronidase
36
. | •Dissolve fibrin clot- aid in bacterial spreading
oFibrinolysin- staphylokinase
37
hydrolyse lipid to ensure survival in sebaceous areas of the body
Lipases
38
oPenicillinase | •
Plasmid
39
- antibacterial lipid- prolonged bacterial survival
oFatty acid modifying enzyme (FAME)
40
Epidemiology
Transient colonizer of skin
41
Epidemiology Nasal carrier
Anterior nasopharynx
42
Epidemiology Persistent carrier
Hospital personnel
43
Ritters Disease or SSSS Pathophysiology
``` Perioral erythema to Spread to Body to Bullous to Desquamation ```
44
Ritters Disease or SSSS Sign
Nikolsky sign
45
Ritters Disease or SSSS Localized from of SSSS
Bulbous impetigo
46
Ritters Disease or SSSS Bullous impetigo
Localized blister | Culture positive
47
TSS Growth of organism in vagina or wound
Release of TSST -1
48
TSS Manifestations
``` Fever Macular erythematous Hypotension Multi organ involvement Desquamtion of Palm and sole ```
49
Cutaneous infection
``` Impetigo Folliculitis Furuncle Carbuncle Wound infection ```
50
Others
``` oBacteremia oEndocarditis oPneumonia oEmpyema oOsteomyelitis oSeptic arthritis ```
51
S. Aureus is positive from
Alpha toxin (most strains) Acid from mannitol Coagulase reaction DNase production (usually)
52
S aureus pigment production
Usually golden
53
S epidermis pigment production
White
54
S. Saprophiticus
White
55
S. Epidermidis and CNS
oEndocarditis- native or artificial valves oCatheter and shunt infection oProsthetic joint infection oUTI
56
Laboratory diagnosis Grow rapidly within
24 hours
57
Laboratory diagnosis Blood agar
Hemolysis
58
Laboratory diagnosis Selective media
Add NaCL 7.5%
59
Laboratory diagnosis Mannitol
Fermented by S. Aureus
60
Laboratory diagnosis Catalase test
3% H2O2 bubbles
61
Treatment Semisynthetic penicillinase resistant penicillin
oOxacillin/Cloxacillin oNafcillin oClindamycin o1st Gen cephalosporins
62
Resistance MSSA gene
None
63
Resistance gene MRSA
Me gene | SCCmec
64
Resistance gene VISA
None
65
Resistance gene VRSA
Van A
66
Resistance comment MSSA
Oxacillin
67
Resistance comment | MRSA
Vancomycin sensitive MIC 2
68
Resistance comment VISA
Quinopristine dalfopristine oxazolidinone MIC > 4-8
69
Resistance comment VRSA
MIC > 16
70
Resistance Betalactamase
Penicillin
71
Plasmid mediated
Tetracycline Erythromycin Aminoglycosides
72
Tolerance
Inhibit growth | Not killed
73
Staphylococcus
oNon motile, non sporeformer oAerobic or Facultative Anaerobic oFerment CHO to lactate oCatalase positive

Microbiology (6 decks)

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