Subgroups And Discrepancies

Question 1

Mixed field woth anti-A and Anti A,B

Answer 1

A3

Question 2

Weak agglutination with anti-A,B

Answer 2

Ax

Question 3

> /=10 red blood cells show very weak mf agglutination

Answer 3

A end

Question 4

No agglutination with anti-A and anti-A,B

Answer 4

Am, Ay, Ael

Question 5

No dectection of anti A and anti a,b can be detected by

Answer 5

Adsorption-elution

Question 6

Mixed field B

Answer 6

B3

Question 7

Agglutination with Anti-A,B (wk/o with anti-B)

Answer 7

Bx

Question 8

No agglutination with anti-B and anti-A,B ( B subgroup)

Answer 8

Bm and Bel

Question 9

Weak and missing antibodies

Answer 9

Group 1

Question 10

Weak and missing antigen

Answer 10

Group 2

Question 11

Group 1 Discrepancies

Answer 11

Newborns
Malignancy ( CLL)
Hypo/Agammaglobulinemia
ABO subgroups

Question 12

Group 2 discrepancies

Answer 12

Subgroups of A&/B
Leukemia (ALL)
Hodgkins and disease
Acquired B phenomenon ( use monoclonal anti-B clone ! treat using acetic anhydride)

Question 13

Resolution for ABO Group 1&2 Discepancies

Answer 13

Use 2-5% RBC
Incubate sample and reagent @room temp for 15-30 mins if negative incubate at ref temp for 15 minutes

Question 14

Group 3 discrepancies

Answer 14

Whartons jelly
Globulins(MM,WM)
Increased fibrinogen
Plasma expanders

Question 15

Rouleaux

Answer 15

Group 3 discrepancies

Question 16

Miscellaneous

Answer 16

Group 4 discrepancies

Question 17

Group 4 discrepancies

Answer 17

Polyagglutination
Anti-I (cold agglutinin
CIS-AB Phenotype
Unexpected ABO isoagglutinin (Anti-H/Anti-A1)

Question 18

Group 3 resolution

Answer 18

Wash RBC w/saline several times (6-8x)

Question 19

Group 4 resolution

Answer 19

Incubate/wash with saline at 37
Treat w/ 0.010M DIT