SYLLABUS 8: Fatty acid oxidation & ketone body production Flashcards Preview

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Flashcards in SYLLABUS 8: Fatty acid oxidation & ketone body production Deck (38):
1

what does oxidation of fatty acids provide

9 Kcal/g of energy 

2

where are fatty acids important

major source of energy for most tissues; but not RBC or BRAIN 

major storage form of energy, as triglycerides 

 

3

where does out dietary fat come from

triglycerides, 3 fatty-acid esters of teh alcohol, glycerol 

 

4

general chemical structore of a fatty acid? 

CH3 - (CH2)n - COOH 

 

5

structure of triglycerides?

glycerol backbone 

3 fatty-acids in ester linkage

6

size of most fatty acids in our diet?

16 to 18 carbons 

some contain double bonds in methylenes = unsaturated fatty acids - i.e. oleic acid 

if >1 double bond, = polyunsaturated fatty acids, i.e. linoleic acid 

7

what are fatty acids synthesized from 

acetyl CoA 

which came from pyruvate, which came from glucose 

requires lots of energy to make fatty acids - NADPH - from pentose cycle, shuttles 

 

8

what is the function of lipases in our diet?

what organs produce lipase?

what organs are involved w/ its action?

pancreas produces lipase;

lipase hydrolyzes dietary triglycerides to fatty acids + glycerol 

bile salts from liver aid this

 

9

function of adipose tissue re: triglycerides?

fatty acids and glycerol are taken up by adipose tissue 

triglycerides are resynthesized and stored largely in adipose tissue

10

functions of fatty acids?

1. component of phospholipids & glycolipids of biological membranes 

2. major energy source for most tissues 

3. major storage form of energy as triglycerides 

4. produce signal transduction molecules, eg inositol phosphates, diacylglycerol 

5. produce prostaglandins and leukotrienes

11

when are fatty acids the main fuel for tissues?

in the fasted state 

 

12

what activates or inactivates lipase? result re: fatty acids?

in the FASTED STATE: glucagon, epinephrine, norepinephrine activate lipase via cAMP-dependent PKA phosphorylation 

 

in the FED STATE: insulin inhibits lipase by dephosphorylation

 

 

13

where does fatty acid oxidation take place?

mito & some peroxisomes

14

describe process of Fatty Acid oxidation in Mito and Peroxisomes, before B-oxidation

1. glucagon, epi, or norepi activate the hormone-sensitive lipase by cAMP-dependent PKA phosphorylation 

2. activate lipase hydrolyses stored triglycerides to glycerol + fatty acids

 3. glycerol exits adipose tissue, goes to liver, enters gluconeogenesis or glycolysis at G3P & DHAP level 

4. fatty acids also can bind albumin in blood, transport to tissues; but albumin-fatty acid complex can't cross blood brain barriers, so fatty acids do not reach brain for oxidation

5. fatty acids are transported from albumin to fatty acid binding proteins for delivery inside the tissue

 

15

pathway of fatty acid oxidation? 

B-oxidation pathway 

 

16

once fatty acid is in the cell, how does it get into the inner mito membrane?

1. fatty acid is brought to cell membrane by fatty acid binding protein 

2. fatty acid is activated to the fatty acyl CoA ester by acyl CoA synthase 

this involves exchange of ATP + CoA for AMP + Pi 

if short or medium chain acyl CoA synthase, fatty acid can directly enter the mito 

if long, need a carnitine shuttle to enter 

3. carnitine shuttle converts long-chain fatty acyl CoA to long-chain fatty acyl carnitine

4. long-chain fatty acyl carnitine enters the mito via carnitine translocase carrier

5. in mito, fatty acyl carnitine interacts w/ carnitine transferase 1 or 2 to regenerate carnitine & fatty acyl CoA

6. fatty acyl CoA undergoes B-oxidation

carnitine is shuttled out of matrix by carnitine translocase carrier, so it can do more carries of fatty acyl CoA into mito matrix 

 

 

17

what fatty acids need carnitine shuttle? fxn?

brings fatty acids from long chain fatty acyl CoA synthases (>12 C) into the inner mito matrix 

short or medium chain (4-8 C or 8-12 C) do not need carnitine shuttle

18

what is B-oxidation? products of it?

a series of 4 enzyme catalyzed reactions that acyl CoA undergoes

splits a 2C fragment of the acyl CoA to produce acetyl CoA + new fatty acyl CoA shortened by 2 C atoms 

reaction produces 1 NADH and 1 FADH2 in steps 1 and 3 of the rxn

19

how long does the B-oxidation process continue for?

it's a spiral, repeats until all the carbons are oxidized to acetyl CoA 

each spiral produces 1 FADH2 and 1 NADH 

 

20

how many times would an 18 C fatty acid undergo B oxidation?

products?

8 spirals 

this produces 8 NADH, 8 FADH2, and 9 2C acetyl CoA products

21

how are odd chain fatty acids differently oxidized in B oxidation?

products are acetyl CoA (2C product) and propionyl CoA (3C product) 

propionyl CoA undergoes 3 step metabolism reaction to become succinyl CoA 

1. propionyl CoA acted on by biotin produces D-Methyl malonyl CoA

2. MMA epimerase acts on D-MM CoA, produces L-MM CoA 

4. MMA mutase, using B12, acts on L-MM CoA, produces succinyl CoA

 

22

what regulates the rate of B-oxidation?

1) availability of fatty acids via activated hormone-sensitive lipase

2) availability of carnitine 

3) malonyl CoA is the rate-limiting step of fatty acid synthesis, inhibts carnitine acyl transferases 1 and 2

4) rate of the electron transport chain

23

what controls the availability of carnitine?

1) inborn errors of metabolism which lower carnitine synthesis 

2) lysine synthesizes carnitine in mammals 

 

24

how many ATPs are produced by B-oxidation of a C18 fatty acid?

8 NADH -> 20 ATP 

8 FADH2 -> 12 ATP 

9 acetyl CoA -> 90 ATP 

- 2 ATP used to activate the fatty acid 

= net ~120 ATP prouced 

25

what happens to acetyl CoA if the TCA cycle isn't functioning? 

i.e. if OAA is depleted by gluconeogenesis?

it cannot enter the TCA cycle 

do not want this acetyl CoA to pile up, as this would deplete CoASH and fatty acid oxidation wouldn't continue

liver (and small extent kidneys) do ketone bodies formation / ketogenesis to metabolize actyl CoA 

26

when isn't OAA available

gluconeogenic conditions, since then OAA is pulled out of the mito to make glucose 

27

where does ketogenesis occur

the liver mitochondria

28

describe the process of ketogenesis?

overall, 2 acetyl CoAs are converted to acetoacetate 

occurs in the mitochondria 

1. acetyal CoAs -> acetoacetyl CoA, by thiolase

2. acetoacetyl CoA -> HMG COA, by HMGCoA synthase 

3. HMGCoA -> Acetoacetate + Acetyl CoA, by HMGCoA lyase 

4. Acetoacetate -> B-OH butarate or -> acetone + CO2 (by spontaneous decarboxylation)

29

on whom is acetone detectable? why?

breath of diabetics, fasting individuals, alcoholics 

because acetoacetate is produced from ketogenesis, and this spontaneously decarboxylates to acetone + CO2; an spell acetone on these ppl 

30

what happens to the products of ketogenesis?

they leave the mito of the liver on carrier 5; leave the liver; go to the blood; go to different tissues, where they're converted back to acetyl CoA, oxidized in the TCA cycle

31

effect of high levels of ketone bodies in the blood?

= ketosis

ketone bodies are strong aids 

alter (lower) blood pH greatly 

 

 

32

who has ketosis?

diabetics, poorly nourished alcoholics 

33

where do ketone bodies made in liver supply energy to?

othe rtissues 

esp muscle, heart, kidney cortex

34

does brain oxidize ketone bodies?

when/if?

no 

because the transferase that converts acetoacetate to acetoacetyl CoA isn't present in the brain 

ONLY under conditions of stress i.e. starvation, which allows brain to oxidize ketone bodies, use them for fuel - lowers brain's need for glucose utilization

35

what happens to the products of ketogenesis?

are converted to acetyl CoA which circulates in blood, goes to other tissues, undergoes teh TCA cycle and makes energy 

36

what is the peroxisomal fatty acid system?

makes some acetyl CoA

preferentially oxidized very long chain fatty acids of C22-26, to median chains, which will go to the mito, where they're oxidized 

produces acetyl CoA and NADH, NO FADH2 - instead, O2 is used as an e- acceptor, producing H2O2 which peroxisomal catalse removes

37

how do the products of peroxisomal fatty acid oxidation enter the mito?

1) acetyl CoA enters the mito as acetyl carnitine, where it's oxidized in the TCA cycle 

2) NADH is transported into mito by malate-aspartate shuttle 

3) spiraling continues til medium chain fatty acid's produced, which enters mito B-oxidation pathway 

38

what induces the peroxisomal fatty acid oxidation pathway?

clofibrate and fibrates, drugs that are used to lower serum lipid levels by enhancing peroxisomal fatty acid oxidation

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