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Microbiology Module 2 > T1: Anaerobic GPRs > Flashcards

T1: Anaerobic GPRs Flashcards

(59 cards)

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1
Q

Probrionibacterium

- normal flora

A

skin

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2
Q

Probrionibacterium

- GLC pattern

A

GLC = aP

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3
Q

Probrionibacterium

- What infections is it involved in

A

CNS shunt infections, endocarditis, prosthetic joint infections, eye
- Found in blood and CSF cultures after 5 days

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4
Q

Bifidobacterium

- Normal Flora

A

Common fecal flora

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5
Q

Bifidobacterium

- Gram stain

A

Y shaped GPR

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6
Q

Lactobacillus

- normal flora

A

GI and vaginal flora

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7
Q

Actinomyces

- Gram stain

A

often branching GPRs

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8
Q

Actinomyces israelii

- plate morphology

A

ROUGH/SLOW growth, molar tooth 5- 14 days or longer

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9
Q

Actinomyces israelii

- gram stain

A

branching rods

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10
Q

Actinomyces israelii

- broth

A

chunky in broth

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11
Q

Actinomyces israelii

- disease

A
  • Actinomycosis - “Lumpy Jaw”

- Sulphur granules from drainage

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12
Q

Actinomyces naeslundii

- plate morphology

A

Moderate growth/SMOOTH on BAP

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13
Q

Actinomyces naeslundii

- broth

A

turbid

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14
Q

Mobiluncus

- Gram stain

A

motile slightly curved rod

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15
Q

Mobiluncus

- disease

A

non-specific vaginitis or bacterial vaginosis

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16
Q

Eubacterium

- disease

A

rarely cause disease because beneficial bacterium

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17
Q

Eubacterium

- normal flora

A

intestinal tract

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18
Q

Eggerthella lenta

- normal flora

A

intestinal tract

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19
Q

Three types of spores

A

Terminal
Central
Subterminal

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20
Q

Most clinical isolates of anaerobic gram positive spore forming rods are what?

A

Clostridium

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21
Q

Clostridium perfringens

- gram stain

A

GPR - spore forming but often stains gram neg on a direct smear

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22
Q

Clostridium perfringens

- What are the reactions with milk, lecithinase, lipase, and reverse CAMP test?

A

milk: proteolytic (milk-curd/digest)
Lecithinase: positive
Lipase: negative
Reverse CAMP: positive

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23
Q

Clostridium perfringens

- plate morphology

A

double zone beta hemolysis

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24
Q

Clostridium perfringens

- two major diseases

Study These Flashcards
A
  1. Gas gangrene

2. Food poisoning

25
Gas gangrene (myonecrosis) - most common cause - toxins - what happens
- Clostridium - Cytotoxins destroy cells/tissues - Gas, bullae, discoloration
26
Crepitant (anaerobic) cellulitis | - what is not involved
muscle and facia not involved
27
Clostridium perfringens food poisoning is found in what foods?
meats and gravy
28
Clostridium ramosum | - flora
GI
29
Clostridium ramosum | - Gram stain
virginia rail fence and palisading; terminal spores rarely seen
30
Clostridium septicum | - plate morphology
swarms; spreading colonies; slight beta hemolysis
31
Clostridium septicum | - gram stain
GPR that may have citron-shaped GPRs
32
Clostridium septicum | - two diseases
1. gas gangrene | 2. bacteremia associated with malignancy
33
Clostridium septicum | - for diseases, are the mortality rates high or low and can therapy make a difference
- High | - early recognition and proper therapy can make a significant difference in mortality
34
Clostridium sordellii | - gram stain
chaining GPR, with lots of free spores
35
Clostridium sordellii | - Plate morphology
very irregular colonies
36
Clostridium tetani | - gram stain
GPRs with LARGE terminal spores
37
Clostridium tetani | - plate morphology
Irregular shaped colonies, "course ground glass"
38
Tetanus - another name/ what does it cause - infection or intoxication
- Lockjaw | - intoxication
39
Clostridium botulinum | - three infections
1. Foodborne 2. Infant botulism (floppy baby syndrome) 3. Wound botulism
40
Foodborne C. botulinum - found in what foods - infection or intoxication - what occurs from disease - treatment
- home canned foods - intoxication - descending "flaccid paralysis" - Antitoxin
41
Infant botulism causes by C. botulinum - another name - what food is implicated - infection or intoxication
- Floppy Baby Syndrome - Honey - Infection
42
Clostridium sporogenes | - lipase reaction
positive
43
Clostridium sporogenes | - gram stain
GPR with subterminal and free spores
44
Clostridium sporogenes | - colony morphology
can swarm (filamentous, medusa head colonies)
45
Clostridium difficile | - normal flora
intestinal flora
46
Clostridium difficile | - plate morphology
Large colonies, irregular edge and shape, raised yellowish to grey-white with a COARSE "CIS"
47
Clostridium difficile | - GLC
GLC = a...IC (lots of peaks in-between)
48
C. difficile disease an _______ ________ diarrhea
antibiotic associated
49
What is true about C. difficile disease's mortality
bloody diarrhea can be fatal
50
Why does C. difficile disease potentially serious threat to immunocompromised patients?
spores on hands, bed sheets, surfaces in hospital environment
51
Clostridium difficile | - two major toxins
1. Toxin A = enterotoxin | 2. Toxin B = cytotoxin
52
Two ways to identify Clostridium difficile
1. Rapid real-time PCR | 2. Gold standard toxigenic culture for C diff and ID - but too slow
53
Rapid real-time PCR assays for which of Clostridium difficile's toxins
Toxin B
54
Clostridium clostridioforme | - gram stain
GPR that more commonly stains gram negative; tapered ends - football shaped
55
Indole | - what does a positive and negative reaction look like
Postive: red color Negative: no color change
56
Gelatin Test | - how do you perform the test?
Gelatin liquefaction. Place tube in refrigerator for 20 minutes and check for liquefaction
57
Gelatin Test | - Postive and negative reactions
Positive: liquid Weak pos: semisolid or partial liquid Negative: solid
58
Milk test | - what are we testing for
milk proteolysis by visually inspection
59
Milk test | - results
Milk is solid: CURD Milk is clear: DIGESTION Milk is solid w/ some clearing: Curd and Digestion Milk has no change: NC

Microbiology Module 2 (19 decks)

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