T2: Anaerobic, GNCs, GPCs, and GNRs

Question 1

What is the most commonly isolated GNC?

Answer 1

Veillonella

Question 2

Veillonella

- gram stain

Answer 2

small GNC

Question 3

Veillonella

- colonial morphology

Answer 3

circular, raised, smooth, and creamy

Question 4

Veillonella

- Normal flora

Answer 4

oral, respiratory, intestinal, genitourinary…(mucous membrane lined cavities)

Question 5

Veillonella

- Nitrate reaction

Answer 5

Nitrate positive

Question 6

Peptococcus niger

- pigment

Answer 6

black to olive green - pigment fades with oxygen exposure

Question 7

Peptococcus niger

- Gram stain

Answer 7

GPC but often gram variable

Question 8

Finegoldia magna

- gram stain

Answer 8

large GPC

Question 9

Finegoldia magna

- normal flora

Answer 9

intestine

Question 10

What is the most pathogenic of the Ana GPC’s and most frequently isolated in pure cultures?

Answer 10

Finegoldia magna

Question 11

Parvimonas micra

- Gram Stain

Answer 11

small GPC

Question 12

Parvimonas micra

- colonial morphology

Answer 12

Circular, raised, smooth, white to grey, often SLIGHT BETA HEMOLYSIS

Question 13

Parvimonas micra

- normal flora

Answer 13

mouth/upper rest. tract

Question 14

Peptostreptococcus anaerobius

- Gram Stain

Answer 14

elongated GPC

Question 15

Peptostreptococcus anaerobius

- Colonial Morphology

Answer 15

circular, raised, smooth, white to creamy

Question 16

Peptostreptococcus anaerobius

- GLC

Answer 16

a…IC

Question 17

Peptostreptococcus anaerobius

- Normal Flora

Answer 17

nose, throat, intestine

Question 18

Peptostreptococcus anaerobius

- SPS disk test

Answer 18

> 12 mm zone of inhibition (susceptible)

Question 19

What commonly shows up in blood and CSF cultures?

Answer 19

Staphylococcus saccharolyticus

Question 20

What is the #1 brain abscess isolate?

Answer 20

Streptococcus intermedius

Question 21

Fusobacterium

- normal flora

Answer 21

mouth and colon

Question 22

Fusobacterium

- associated with infections of what?

Answer 22

infections of the mouth (human bites, lung abscesses, pleural cavity, brain abscess)

Question 23

Fusobacterium

- GLC

Answer 23

Major butyric peak

GLC = aB

Question 24

Fusobacterium nucleatum

- Gram Stain

Answer 24

GNR - fusiform shaped rods - thin tapered ends

Question 25

Fusobacterium nucleatum | - colonial morphology

Answer 25

circular to slightly irregular, raised, coarse ground glass appearance, yellowish or pink and green iridescence

Question 26

Fusobacterium nucleatum | - indole reaction

Answer 26

positive

Question 27

Fusobacterium nucleatum | - two forms

Answer 27

Smooth: mottled (course ground glass), pinkish to greenish | Molar tooth forms

Question 28

Fusobacterium necrophorum | - Gram stain

Answer 28

GNR, short and long forms, NOT fusiform shaped on gram stain

Question 29

Fusobacterium necrophorum | - Colonial morphology

Answer 29

"fried egg" colonies (raised yellow center)

Question 30

Fusobacterium mortiferum | - normal flora

Answer 30

colon

Question 31

Fusobacterium mortiferum | - Resistant to what?

Answer 31

antibiotics

Question 32

Fusobacterium mortiferum | - Colony morphology

Answer 32

colonies have whirl appearance

Question 33

Fusobacterium mortiferum | - Gram stain

Answer 33

bizarre pleomorphic forms from broth (spaghetti and meatballs)

Question 34

Bacteriodes spp is broke up into two groups, what are these groups?

Answer 34

Indole negative and indole positive

Question 35

2 Bacteroides species in the Indole negative group

Answer 35

B. fragilis | B. vulgatus

Question 36

2 Bacteroides species in the indole positive group

Answer 36

B. ovatus | b. thetaiotamicron

Question 37

Bacteroides fragilis group | - normal flora

Answer 37

colon

Question 38

Bacteroides fragilis group - stimulated by what - resistant to what disk

Answer 38

- stimulated by 20% BILE | - bile disk resistant

Question 39

Bacteroides fragilis group | - GLC

Answer 39

ap (ib) iv

Question 40

Bacteroides fragilis group | - producer of what, making it very resistant to what?

Answer 40

penicillinase producer, very resistant to antimicrobials

Question 41

Bacteroides fragilis group | - Gram stain

Answer 41

Large gray -black colonies on BBE agar and growth on LKV after overnight incubation

Question 42

Bacteroides fragilis | - gram stain

Answer 42

GNR, short regular rods, broth cultures are pleomorphic

Question 43

Bacteroides fragilis | - colonial morphology

Answer 43

fine ground glass or CIS

Question 44

Bacteroides thetaiotaomicron | - gram stain

Answer 44

GNR, short and longer forms, pleomorphic in broth

Question 45

Bacteroides thetaiotaomicron | - colonial morphology

Answer 45

circular, raised, large, shiny, mucoid, white to grey, often with striations

Question 46

Which is the second most common bacteroides?

Answer 46

Bacteroides thetaiotaomicron

Question 47

Porphyromonas | - pigment

Answer 47

pigmented black

Question 48

Porphyromonas | - indole reaction

Answer 48

postive

Question 49

Porphyromonas | - growth on LKV?

Answer 49

no growth

Question 50

Porphyromonas | - normal flora

Answer 50

mouth

Question 51

What bacteria are synergistic (usually found with other organisms), require hemin and vit K in vivo, and produce beta lactase?

Answer 51

Prevotella species

Question 52

Brick red fluorescence, and pigment make protoporphyrin - dark pigment that causes the colonies to become brown/black

Answer 52

Prevotella melaninogenica

Question 53

Prevotella oralis | - normal flora

Answer 53

mouth

Question 54

Prevotella bivia and P. disiens | - normal flora

Answer 54

vaginal flora

Question 55

Campylobacter ureolyticus | - gram stain

Answer 55

pitting of agar

Question 56

Campylobacter ureolyticus | - urease reaction

Answer 56

urease positive