Test 3 Flashcards
(41 cards)
Photosynthesis
- Series of energy conversion reactions (solar energy into usable form)
- Plant use visible light for photosynthesis
- Occurs in the chloroplast
Photon
Unit of the electromagnetic spectrum for photosynthesis
-Packet of energy that travels in waves and measured in wavelengths
Radio wave (long wavelength) Gamma rays (short wavelength)
- Shorter wavelength = higher energy
- Higher wavelength = lower energy
- VIsible light: (380-760)
- Violet (short wave) - red (long wave)
Chloroplast
- outer/inner membrane then thylakoid membrane
- lipid bilayer
Granum
Stacked thylakoids
Free thylakoids
Thylakoids floating in the stroma
Lumen
Inside the thylakoid membrane
Stroma
Outside the thylakoids membrane; aqueous matrix
Light reactions
- Energy conversions
- First steps to occur in photosynthesis
- Occur on thylakoid membrane
- e transport chain
- light energy (photons) -> chemical energy (ATP, NADPH)
Calvin-Benson cycle
- Second step to occur in photosynthesis
- Occur in stroma
- CO2 (PE low) -> Carbs/sugar (PE high) energy requirement, from light rxs (ATP, NADPH)
- E input (from light rxn): ATP, NADPH
- E output; ADP, NADP+
Pigments (hydrophobic)
- Embedded in thylakoid membrane in chloroplasts.
Chloroplasts
- Chl A and B ( used on land plants only)
- Carotenoids ( accessory pigments): yellow, red, orange pigments
- These pigments are anchored in specific structures called photosystems (PS II and I)
Photosystems (II and I)
- Pigment protein complexed that harvest light inside the thylakoid membrane
- Majority happens in the attena complex
Attena complex
Contain accessory pigments, carotenoids, chlorophyll B
Reaction center
Contains 2 chlorophyll A molecules that can donate e for the e transport chain
Steps in light/electron harvesting for photosystems
- Sunlight strikes electron and excites it
- Electron then jumps up to another energy level (pinball)
- e fall back to ground state and excite the accessory pigments located in the antenna complex
- They will then illuminate the e and keep passing e until they reach the reaction center that contain the chl A e
- The chl A will start redox reactions and donate e to an e receptor
Plastoquinone
- e- delivery guy
- located inside the lipid bilayer
- picks up charged e
Plastocyanin
e shuttle in lumen and replenishes e- for PS1
Lumen
- Has a high proton concentration
- stroma has a low proton concentration
Electron transport chain (light reaction steps)
PHS II
- Light strikes an energy, exciting it. It bumps into another e- and pinball reaction until it reaches chlorophyll A in reaction center
- Photochemistry occurs; an e- will be taken off of EACH of the chlorophyll molecules. This is an oxidation rxn. At the same time, reduction is occurring from the Plastoquinone accepting the donated e-.
a. (the plastoquinone is (+) charged, it must neutralize the 2- charged e- it picked up. It does this through grabbing 2 (+) charged protons floating in the membrane from the stroma side and bringing it to the lumen side (proton pump in form of PE)
b. The oxygen evolving complex (replenishes e- for reaction center in PS II and is what allows oxygen to be released into the environment) splits H2O
H2O O2 + 2H+ (oxygen is halved); e- from breaking these bonds gets passed to rxn center - After neutralization, the e- gets an H+ and gets transported to the cytochrome. The cytochrome dumps off the extra proton back into the membrane. The H+ are being moved against their concentration gradient from a concentration of highlow (stromalumen), so energy must be inputted
PHS I (Linear e flow; light energy (photons -> NADPH)
- The electrons then get excited through the same procedure from steps 1-3 and ends up at reaction center with 2 chlorophyll A e- and donated to acceptor molecule, the ferredoxin
a. The “dead” e- (low PE) and must be recharged. The e- in plastocyanin are shuttled to reaction center to be reenergized - ferredoxin interacts with ferredoxin NADP+ reductase. Ferredoxin gives e- to reduce NADP+ to make NADPH (redox reaction)
Not part of electron transport chain because no redox reactions taking place: - ATP synthetase (diffusion): H+ will diffuse through and turn the “catalytic knob” to transfer ADP+ + Pi ATP (phosphorylation) using kinetic energy
CYCLIC E- FLOW: involves PS1 and Cytochrome - PS I get excited by light and donates e- to ferredoxin. Ferredoxin reacts with the cytochrome (proton pump) use up all energy so to regenerate energy it goes to plastocyanin who give it back to PS I (rxn center)
a. Used to move proton for a higher proton gradient
Cyclic e flow; involves PS1 and cytochrome
- PS I get excited by light and donates e- to ferredoxin. Ferredoxin reacts with the cytochrome (proton pump) use up all energy so to regenerate energy it goes to plastocyanin who give it back to PS I (rxn center)
a. Used to move proton for a higher proton gradient
Calvin-Benson Cycle
- Occurs in stroma
- Convert inorganic C (CO2) -> organic form (sugar) (c fixation)
- CO2 (PE low) -> carbs/sugar (PE high) energy req from light reactions (ATP, NADPH)
- Energy input: ATP, NADPH
- Energy output : ADP, NADP
- Product (3-C sugar) Glyceraldehyde-3-phosphate
3 phases:
1. Carbon fixation (CO2-> organic C)
2. Carbon reduction (converting C into higher energy form)
3. Regeneration
Photorespiration
- Occurs when oxygen levels increase and CO2 levels decrease
- Recycling of photoglycolate made form oxygenase (RUBP + O2)
- Requires energy
- O2 is released and CO2 is consumed ( when stomata is closed, it prevents O2 release
- Found in. hot temps (tropics) and arid conditions (desert/tundra)