Test 3 Review Flashcards

1
Q

What type of bacteria have porins?

A

Gram negative

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2
Q

If DNA is a ladder, what represents the backbone or sides of the ladder?

A

The sugar-phosphates

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3
Q

In DNA, the 2 strands of nucleic acids are held together by what?

A

Hydrogen bonds between the nitrogenous bases

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4
Q

What is represented by the fluid mosaic model?

A

the structure and function of the cell/plasma membrane

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5
Q

What is a kinase?

A

An enzyme that adds a phosphate group to a molecule

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6
Q

What happens in the catabolism of glucose?

A

Carbon atoms are oxidized

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7
Q

Trimethoprim-sulfamethoxazole (Bactrim) does what?

A

Interferes with folic acid (folate) biosynthesis

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8
Q

How would you describe transcription?

A

RNA polymerase makes a single stranded RNA that is complimentary to one strand of DNA

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9
Q

Where is a series of redox reactions in the membrane used?

A

The electron transport chain

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10
Q

What is Oxidative phosphorylation?

A

When ATP is made in the electron transport chain

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11
Q

Which pathway makes ATP via substrate level phosphorylation?

A

Glycolysis and the TCA/Krebs Cycle

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12
Q

What does it mean if a carbon has a lot of electrons orbiting around it?

A

The carbon is very reduced

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13
Q

Which biomolecule usually makes up enzymes in metabolism?

A

Proteins

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14
Q

The TCA cycle does all of the following except:

A

Make ATP by substrate level phosphorylation
Makes lots of reduced coenzymes
Oxidizes carbon atoms from glucose
[Makes ATP via oxidative phosphorylation]
Produces CO2 during the pathway

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15
Q

What is true if ATP is made using an electron transport chain?

A

the ATP was made by oxidative phosphorylation

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16
Q

What happens in the preparatory phase of glycolysis?

A

2 ATP molecules are invested

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17
Q

Where is the external terminal electron acceptor used?

A

The electron transport chain

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18
Q

If a bacterial species uses an electron transport chain that only uses elemental sulfur (S) as a terminal electron acceptor, what is true?

A

the bacteria is using anaerobic respiration

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19
Q

What interferes with Peptidoglycan biosynthesis?

A

Penicillin and other beta lactam antibiotics

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20
Q

What do beta lactamases do?

A

Break down penicillin and other betalactams

21
Q

What oxidizes reduced coenzymes?

A

the electron transport chain

22
Q

Which growth phase is where bacteria are the happiest and growing at their optimum rate?

23
Q

How do bacteria evolve by using horizontal gene transmission?

A

Transformation, conjugation, and transduction

24
Q

What is the flow of genetic information between cells?

A

DNA replication

25
If there is no oxygen present and glucose is being catabolized, and there is no ETC, what will happen?
NAD will be produced by reducing pyruvate
26
What are enzymes?
Molecules involved in lowering the energy of activation
27
Why is ATP a high energy compound?
Because it has 3 phosphates next to each other
28
If glycolysis is occurring frequently in a cell, and the cell has to reduce the final partially oxidized end product, what is the reason?
the bacteria has run out of oxidized forms of its coenzymes
29
How can bacteria be identified?
Fermentation end products
30
What is NADH?
Reduced form of the coenzyme NAD
31
What is the production of acetyl CoA from pyruvate by pyruvate dehydrogenase?
an oxidation that produces CO2
32
What are dehydrogenases?
Enzymes that transfer electrons from one molecule to another
33
What is fermentation used to produce?
NAD
34
What is Peptidoglycan biosynthesis an example of?
Anabolism
35
Which produces the most energy?
Substrate level phosphorylation Glycolysis TCA/ Krebs Cycle Oxidative phosphorylation <
36
What is lactic acid an example of?
Fermentation product
37
What is the end result of glycolysis?
Pyruvate
38
Where is the electron transport chain located?
The cytoplasmic/plasma membrane of bacteria
39
What is genetic information flowing between cells an example of?
DNA replication
40
Why is Bactrim effective?
It inhibits one of the metabolic pathways that produces folic acid
41
What does pyruvate dehydrogenase produce?
Acetyl-CoA
42
What is the best way to regenerate NAD for further use?
Using the electron transport chain
43
What happens when electrons are passed down the electron transport chain?
Protons are pumped across the membrane
44
What is Peptidoglycan made of?
Peptides and carbohydrates
45
What process produces fructose-6-PO4?
the preparatory phase of glycolysis
46
What does primase do?
Initiates the synthesis of RNA to provide a 3’OH group for DNA polymerase
47
How are Streptococcus and Neisseria able to take up DNA molecules?
Transformation
48
Where does DNA replication begin?
the origin of replication
49
Which pathogen is the sccMec cassette important for in evolving antibiotic resistance in?
Staphylococcus aureus