The Ell Flashcards
(80 cards)
When was compound microscope made who?
ofcompound microscope
¡Gr-mikros-small, skopein-to see, to look), which was invented by David Jansen in
1590 and in 1610 Galileo an Italian astronomer and physicist designed it properly.
The cell story
The term Cell (Greek, Kytos-cell; La; Cella-hollow space) was first used by
Robert Hooke (1665) to describe his investigations on the structure of cork. Later,
Robert Brown (1831) discovered a spherical body, the nucleus, in the cells of
orchids. The fact that living organisms have a cellular organization was emphasized
by Schleiden (1838) and Schwann (1839).
How did schwann describe cell?
Schwann, observed that the nucleus
was surrounded by a fluid in the cell. His observation thus, changed the definition
of cell and he described the “Cell as a structure which consists of a nucleus
surrounded by a semifluid substance enclosed by a membrane”
“ Later, it was
pointed out, that the structure of a cell in animals and plants is similar with only
one difference, that plant cell has a cell-wall in addition to the cell membrane.
What was the Emergence of Cell Theory?
In 1838, Schleiden, a Gernan botanist, concluded that, despite differences
in the structure of various tissues, plants were made of cells. In 1839, a German
zoologist, T. Schwann, published a comprehensive report on the cellular basis of
animals. Both of them proposed the cell theory which state that:
i)
All organisms are composed of one or more cells.
ii)
The cell is the structural and functional unit of life,
Schleiden and Schwann’s ideas on the origin of cell was not convincing; both
agreed that cell is a ‘de novo’ structure, could arise from non-cellular materials. In
1855 Rudolf Virchow a German pathologist, had made a convincing case and added
a third point to the cell theory.
¡i)
Cells can arise only by division of pre-existing cell, it is not a ‘de novo’
structure
Cell theory brought a great revolution in the field of biology which established
that the function of an organism is the result of activities and interaction of the cell units,
What is a cell?
a cell is defined as the structural and functional unit of living organisms.
made up of protoplasm containing nucleus surrounded by cytoplasm and
bounded by cell membrane.
What r Kinds of microscopes?
Various types of microscopes have been designed by scientist. According to
the source of light following types of microscopes can be recognised.
i)
Light microscope
in which visible light is used as source of
illumination.
¡i)
X-ray microscope
in which short wave length X-rays are
used as source of illumination.
iii) Electron microscope
in which electron beam is used as a
source of illumination.
:
CTOF
Three attributes of microscopes are of particular imp
What is magnification? How much x?
How is it calculated?
Magnification is a means of increasing the
apparent size of the object. With a light microscope a specimen could quite easily be
magnified by as much as 10,000x. Magnification of a microscope is calculated by
multiplying the power of its eye pieces with its magnifying power of its objective.
What is resolution and contrast?
Resolution or more correctly the minimum resolved distance, is the capacity
to separate adjacent form or object. Contrast is important in distinguishing one part
of cell from another. In light microscopes contrast is often obtained by fixing and
staining the material.
How is the resolution for light and electronic microscope?
A very high magnification can be obtained by ordinary light microscope but
their resolving power is limited. It is about 500 times better than unaided human eye,
but this is still not enough for viewing some of the smaller sub-cellular structures.
Electron beams have much shorter wavelength than visible light, electron microscopes
are capable of resolving objects about 10,000 times better than unaided human eye.
Therefore most of the sub-cellular structures are studied by electron microscope.
What is cell fractionation?
Isolation of cellular components to determine their chemical composition, is
called cell fractionation.
How to cell fractionates?
Break/open a large number of similar type of cells in ice cold environment. The cells are
usually placed in a homogenizer or mortor are broken. The freed’ content of the cells
are subjected to a spinning action known as centrifugation. At a low speed, large
particles like, cell nuclei, settle down are in the sediment. Smaller particles are still
in the supernatant (fluid) which can be poured into a fresh tube and subjected to
centrifugation at a higher speed until the smallest particles have been separated out.
the various cell fractions can then be biochemically analysed.
What are eukaryotics?
Eukaryotes are those organisms having a true nucleus (Eu=true,
karyon=nucleus) in their cells. They also contain chromosomes and a variety of
membrane bound organelles like mitochondria, Golgiapparatus, lysosomes, plastids
etc. in their cells, which contribute structural and functional organisation to the
cells. They may be unicellular or multicellular organisms.
What are prokaryotes?
prokaryotes (Pro=early, karyon=nucleus) are those organisms which do not contain
membrane bound nucleus in their cells, their nucleic material is usually coiled and
concentrated in a region of the cell called the nucleoid. More over genetic material
(DNA) never associated with histone protien and hence no true chromosome. These
organisms also do not contain other membrane bounded structures like,
mitochondria, chloroplast, lysosome, etc. in their cells. They only contain mesosomes,
Which are simple infoldings of the Plasma membrane responsible for respiration,
Photosynthesis, nitrogen fixation etc. They are unicellular organisms like bacteria and cynobacteria
What is the plasma membrane?
What other molecules does it contain?
They consist of a double
layer (bilayer) of phospholipids
interspersed with proteins.
The phospholipid molecules in
the plasma membrané are arranged
in two parallel layers, Their non-polar hydrophobic ends face each other, whereas their polar hydrophillic ends
are associated with carbohydrate, protein etc. Plasma membrane also contains
several types of lipids like cholesterol. In certain animal cells cholesterol may
constitute upto 50 percent of the lipid molecules in plasma membrane. It is absent
from the plasma membrane of most plant and bacterial cells.
Who came up with the Fluid Mosaic Model?
In 1972 Singer and Nicolson proposed a working model of plasma membrane
known as fluid mosaic model.
What is the fluid mosaic model
In the fluid mosaic model, the lipid bilayer is retained
as the core of the membrane. These lipid molecules are present in a fluid state apable
of rotating and moving laterally within the membrane. The structure and arrangement of membrane proteins in the Nluid-mosaic
model are like icebergs in the sea. The proteins occur as a ‘mosaic’ of discontinuous
particles that penetrate deeply into and even completely through the lipid sheet. The
components of plasma membrane are mobile and capable of coming together to
engage in various types of transient or semipermanent interaction.
What types of proteins associated with the lipid bilayer?
Two types
Integral proteins (intrinsic proteins)
Peripheral protelns (extrinale protelns)
What is Peripheral protelns (extrinale protelns)?
A class of protein located
entirely outside of the lipid bilayer on either the extracelalar or cytoplasmic
surface, exhibit a loose association with membrane surface.
These proteins which may possess lipid (lipoprotein) or carbohydrate
(glycoproteins) side chains, are arranged as mosalcs with in the cell-membrane.
What are Integral proteins (intrinsic proteins)?
What types of substances can pass through it then?
Integral proteins (intrinsic proteins): A class of proteins that are
directly incorporated within the lipid bilayer. Some of these proteins are believed to
provide a channel through which water-soluble substances, such as ions, can pass
back and forth between the extracellular and intracellular compartment.
Ditferent types of cells contain different population of membrane proteins
Why?
Ditferent types of cells contain different population of membrane proteins 8.g. Red
blood cells have special membrane proteins that label the cell, giving it A, B, AB or
O blood type. Other molecular labels lead to either acceptance or rejection of a
transplanted kidney, heart or other organ.
The plasma membrane performs several functions
What are they?
To maintain what??
The plasma membrane performs several functions but the mar and the most
important functions are protection of cell cytoplasm, to regulate the Now of solutions
and material in an out of the cell with certain limitation. Thesè firitations or check
in flow across the membrane is called differential or selective permeability. Transport
across membranes is necessary to maintain suitable pi, ionic concentration for
enzyme activity and excrete toxic substances etc. For entry or exit there are two main
processes, passive transport i.e. diffusion and osmosis and active transport, the
passive processes do not require energy while active require energy with these, there
are two other phenomenon i.e. endocytosis, and exocytosis.
What is diffusion?
Diffusion: It occurs spontanéously, and no extra energy is required to bring
it about. A few substances freely diffuse across plasma membrane e.g. the respiratory
gases (0, and CO,) diffuse in and out of the cells.
What is osmosis?
Osmosis: It maintains a balance between the osmotic pressure of the
intracellular Fluid and that of interstitial fluid, know as Osmoregulation.
What is active transport?
Active transport: Movement of molecules from lower concentration to
the higher concentration by consuming energy called active transport.