tools of the laboratory #2 Flashcards
(48 cards)
metabolism
all chemical and physical workings of a cell
two types of chemical reactions
catabolism
anabolism
catabolism
degradative breaks the bonds of larger molecules forming smaller molecules releases energy
anabolism
biosynthesis process that forms larger macromolecules from smaller molecules requires energy
enzymes
are biological catalysts that increase the rate of a chemical reaction by lowering the energy of activation
simple enzymes
consits of protein alone
apoenzyme
protein portion
cofactors
nonprotein portion
apoenzymes specificity and active site
active site or specific substrate
metallic cofactors
metal ions
coenzymes
organic factors serve as temporary carrier for some substrates vitamins as the most common
exoenzymes
transported extracellularly where they break down large food molecules or harmful chemicals
endoenzymes
retained intracellularly and function there most enzymes are endoenzymes
constitutive enzymes
always present always produced in equal amounts or at rates regardless of the amount of substrate
regulated enzymes
not constantly present production is turned on in response to changes in the substrate concentration
hydrolysis reactions
catabolic reactions that break down large substates into smaller molecules requires the input of water break bonds.
labile
chemically unstable enzymes
denaturation
weak bonds that maintain the shape of the apoenzyme are broken
metabolic pathways
proceed in a stepwise highly regulated manner to maximize the use of a available nutrients and energy
competitive inhibition
substances that resembles the normal substrate competes with the substrate for the active site
allosteric inhibition
form of competitive inhibition in special types of enzymes with two biding sites
allosteric inhibition 2 sites
the active site and the regulatory or allosteric site
noncompetitive inhibition
inhibitor binds to the entire enzyme-substrate complex and prevents the enzyme from complete its action on the substrate
enzyme repression
automatic suppression of enzyme synthesis when end product builds to excess