Topic 1: Key Concepts In Biology Flashcards Preview

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Flashcards in Topic 1: Key Concepts In Biology Deck (54)
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What’s a eukaryotic cell?

A complex cell. All plants + animals


What’s a prokaryotic cell?

Smaller, simpler cell. Prokaryotes are single celled organisms. Bacteria are prokaryotes.


What’s a nucleus?

Contains genetic material arranged in chromosomes, controls activities of cell


What’s a cytoplasm?

Contains enzymes which control chemical reactions that take place here


What’s a cell membrane?

Holds cell together, controls what enters and exits cell


What’s mitochondria?

Where reactions for respiration (transfers energy cells need to work) take place


What’s ribosomes?

Involved in translation of genetic material in synthesis of proteins


What’s the rigid cell wall?

Made of cellulose, supports and strengthens cell


What’s a large vacuole?

Contains cell sap (weak solution of sugar and salts) maintains internal pressure of cell


What’s chloroplasts?

Where photosynthesis occurs, contain green chlorophyll


What’s chromosomal dna?

Controls activities and replication, floats free in cytoplasm


What’s flagellum?

Rotates to move bacterium away from harmful and towards good substances


What’s plasmid dna?

Small loops of extra dna, can be passed between bacteria, contains genes


What are specialised cells?

Specialised cells have a structure adapted to their function


What’s an acrosome?

At front of sperm cell, stores enzymes to digest membrane of egg cell


How are egg cells specialised for reproduction?

Have nutrients in cytoplasm to nourish early developing embryo
Has haploid nucleus
After fertilisation membrane changes structure


What is resolution?

How well microscope distinguishes between two points thats are close together


How do light microscopes work?

Invented in 1590, they pass light through specimen


How do electron microscopes work?

Invented in 1930, use electrons. Can’t be used to view living cells


Describe the practical ‘how to view a specimen using a light microscope’

1) take things slice (lets light through) of specimen
2, pipette 1 drop of water onto clean slide (secures specimen) and tweezer specimen onto slide
3, add stain to colourless specimen (so you can see it, different stain highlights different structures within cells)
4, hold cover slip at angle using mounted needle, carefully lower onto slide over specimen (So no air bubbles)
5, clip slide onto stage + choose lowest powered objective lens
6, use coarse adjustment knob, move stage up so slide just underneath objective lens. Look down eyepiece, move stage down until specimen almost focused (avoids collision)
7, adjust focus with fine adjustment knob, until image is clear. Position clear ruler on stage, measure diameter of visible circular area
8, greater magnification > higher powered objective lens


Describe the practical ‘scientific drawing of specimen’

1, Outline main features with unbroken lines
2, keep all parts in proportion
3, label important features using straight lines that don’t cross over each other. Include magnification used and a scale.


What is magnification?

How many times bigger the image is


What’s the two equations for total magnification?

Total magnification = eyepiece lens magnification x objective lens magnification
magnification = image size / actual size


Order the units picometer, micrometer, millimetre, nanometre from largest unit of measurement to smallest. How do you convert between them?

Millimetre mm
Micrometer um
Nanometre nm
Picometer pm

Larger > smaller, x 1000
Smaller > larger, divide by 1000


What are enzymes?

Enzymes are biological catalysts that speed up useful chemical reactions in the body without being changed


What’s a substrate?

Molecule changed in the reaction


What’s active site?

Where enzyme joins onto substrate


What do enzymes and substrates have?

Enzymes have a high specificity for their substrate (lock and key)


What’s denaturing?

Some bonds holding enzyme together break, causing shape of enzymes active site to change. Substrate will no longer fit.


Describe the practical ‘investigating effect of pH on enzyme activity’

1, put a drop of iodine solution into every well of a spotting tile
2, place Bunsen burner on heat proof mat, and tripod and gauze over it. Put beaker of water on tripod, heat water to 35* (use thermometer) and try to keep temperature constant throughout experiment
3, use syringe add 3cm^3 of amylase solution and 1cm^3 of a buffer solution with pH 5 to boiling tube. Using test tube holders, out boiling tube in beaker of water, wait 5 mins
4, using different syringe, add 3cm^3 starch solution to boiling tube
5, immediately mix contents of boiling tube and start stop clock
6, record how long it takes amylase to break down all starch using continuous sampling (use dropping pipette, take fresh sample from boiling tube every 10 seconds, put a drop into a well.) When iodine solution remains browny orange, starch no longer present
7, repeat experiment with buffer solutions of different pH values, see how pH affects time taken to break down starch
8, control any variables each time. Could use electric bath to control temperature. Could use pH metre to accurately measure pH of solutions.