transfusion science part 2

Question 1

What is agglutination technique?

Answer 1

In-vitro detection of antigen-antibody reactions

Question 2

What is direct agglutination?

Answer 2

ABO typing technique to see immediate reaction

Question 3

What do the antigens on RBCs do?

Answer 3

Govern blood group

Question 4

What does the plasma do?

Answer 4

Confirm ABO grouping

Question 5

What is the antihuman globulin (AHG) test?

Answer 5

Two types of tests where globulin binds to red cell antibodies

Question 6

Explain direct antiglobulin test (DAT)

Answer 6

Washed red cells
Bound antibodies will be recognised by AHG and results in agglutination

Question 7

Explain indirect antiglobulin test

Answer 7

Detects antibody in patient plasma
Indirect because has period of incubation

Question 8

What is cross matching?

Answer 8

Test to detect incompatibilities between donor and recipient that will not be evident on blood typing

Question 9

When are serological crossmatching performed?

Answer 9

Done if detected antibodies in patient, transplant, fetus and mum transfusion

Question 10

What is the objective of serological crossmatching?

Answer 10

Find which packs of blood from among these in the blood bank fail to agglutinate