Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

[3Y1S] MT 6320 > U2.1.1 L : Experiment 6-7 > Flashcards

U2.1.1 L : Experiment 6-7 Flashcards

1
Q

Differential Staining

Principle : used to distinguish if the isolate is Gr (+) or Gr (-)

A

Gram Staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Gram Staining

Gram (+) bacteria : color

A

stained purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Gram Staining

Gram (-) bacteria : color

A

stained red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

T/F Gram Staining is based on the reaction of bacterial cell wall on staining solutions

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Two Types of Smear

prepared from colonies growing from plated media

A

Indirect Smear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Two Types of Smear

prepared from clinical specimen

A

Direct Smear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Staining Procedure Gram Stain
Stain :
Mordant :
Decolorizer :
Counterstain :

A

Stain : Crystal Violet for 1 minute
Mordant : Gram’s Iodine for 1 minute
Decolorizer : Acetone-alcohol or 95% Ethanol for 10 seconds
10 seconds
Counterstain : Safranin O for 30 seconds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

T/F In Gram staining smear should have pink/red or clear background and cells are overlapping

A

F, cells should not be overlapping

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Gram staining

Examine smear _____/____ scanning

A

Horizontal / Vertical Scanning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Microscopic Examination : Direct Staining

Microscopic Structures Present

A

Epithelial Cells, PMNs, RBCs, Host cellular material

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Gram Staining

Microscopic Examination : Enumeration of Cells under LPO

1+

A

1+ = </LPF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Gram Staining

Microscopic Examination : Enumeration of Cells under LPO

2+

A

2+ = 1-9/LPF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Gram Staining

Microscopic Examination : Enumeration of Cells under LPO

3+

A

3+ = 10-25/LPF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Gram Staining

Microscopic Examination : Enumeration of Cells under LPO

4+

A

4+ = >25/LPF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Gram Staining

Microscopic Examination : Enumeration of Bacteria under OIO

1+

A

1+ (rare or occasional) = <1/OIF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Gram Staining

Microscopic Examination : Enumeration of Bacteria under OIO

2+

A

2+ (few) = 1-5/OIF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Gram Staining

Microscopic Examination : Enumeration of Bacteria under OIO

3+

A

3+ (moderate) = 6-30/OIF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Gram Staining

Microscopic Examination : Enumeration of Bacteria under OIO

4+

A

4+ (heavy) = >30/OIF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Differential Staining

Principle : For the detection of Tubercle bacilli in sputum specimen

A

AFS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

AFS

What does it detect in sputum specimens

A

Tubercle bacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

T/F In AFS, Tubercle bacili resist decolorization w/ acid-alcohol and appear red on a blue / green background after decolorization

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

AFS

Causative agent of Mycobacterium Tuberculosis

A

Tubercle bacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

AFS

Primary diagnostic and monitoring tool for TB

A

Direct Sputum Smear Microscopy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

AFS

Specimen Considerations
Time

A

Collected preferably early morning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
AFS Sputum should be ____
Sputum should be thick, cloudy, and sticky
26
AFS Procedure Smear should be spread properly showing ____ patterns
concentric / coil-like patterns
27
AFS Stain
0.3% Ziehl’s Carbol Fuchsin
28
AFS Decolorizer
Acid alcohol
29
AFS Counterstain
0.3% Methylene blue / Malachite Green
30
AFS Microscopic Examination Manner of scanning
From left to right go downward a bit and then right to left
31
AFS Microscopic Structures Present
Squamous Cells, Leukocytes, Mucus Threads, Bacterial cells aside from AFB
32
AFS Color of Bacterial Cells aside from AFB
Blue and green
33
AFS Color & Morphology of AFB
red or pink bacilli; thin, filamentous or beaded
34
Enumeration of AFB under OIO +n
+n : 1 - 9 AFB seen in 100 OIF
35
Enumeration of AFB under OIO 1+
1+ : 10-99 AFB seen in 100 OIF
36
Enumeration of AFB under OIO 2+
2+ : 1-10 AFB/OIF in at least 50 fields
37
Enumeration of AFB under OIO 3+
3+ : >10 AFB/OIF in at least 20 fields
38
Special Staining T/F Pathogenic organisms have a protected covering made up of polysaccharide polymer and polypeptide
T
39
Special Staining Stain used for Capsules
India ink / Nigrosin
40
Special Staining Appears halo-like against stained amorphous background
Capsule
41
Special Staining A virulence factor which inhibits phagocytosis
Capsule
42
Special Staining : Capsule Specimen
Klebsiella pneumoniae on a nutrient agar slant or plate
43
Special Staining multilayered ovoid or spherical structures present inside Gr (+) bacteria
Endospores
44
Special Staining Seen among Bacillus and Clostridium
Endospore
45
Special Staining Organisms with endospores
Bacillus & Clostridium
46
Special Staining Can be found centrally, subterminal, or terminal
Endospore
47
Special Staining : Spore T/F Endospores are highly resistant to heat, desiccation, and chemical disinfectant
T
48
Special Staining : Spore Main component of spore
Calcium dipicolinate or dipocolonic acid calcium complex
49
Special Staining : Spore appear as _____ structure
clear structure
50
Special Staining : Spore Technique used
Schaeffer-Fulton Method
51
Special Staining : Spore Specimen
Pure colonies of Bacillus subtilis in a nutrient slant or plate
52
Special Staining : Spore Stain used
6.5% Malachite green
53
Special Staining : Spore Counterstain + time
Safranin O for 30 seconds
54
Special Staining : Spore Endospores stain ____
green
55
Special Staining : Spore Vegetative cells stain ____
red/pink
56
Special Staining stained using Leifson staining technique
Flagella
57
Special Staining : Flagellar most common stain used
Leifson staining method
58
Special Staining : Flagellar Leifson staining technique Primary Stain
Basic fuchsin
59
Special Staining : Flagellar Leifson staining technique Mordant
Tannic Acid
60
Special Staining : Flagellar Leifson staining technique Counterstain
Methylene Blue
61
Special Staining : Flagellar Properly prepared bacterial suspension = [appearance]
slightly cloudy
62
Special Staining : Flagellar when do you wash the smear with distilled water [2 indicators]
1. golden precipitate is formed on the dye surface 2. precipitates appear on the smear
63
Special Staining : Flagellar color of bacterial cells and flagella
red
64
Special Staining : Flagellar devoid of flagella
Atrichous
65
Special Staining : Flagellar flagella on one pole/end
Monotrichous
66
Special Staining : Flagellar a tuft/group of flagella on one pole/end
Lophotrichous
67
Special Staining : Flagellar flagella on both poles/ends
Amphitrichous
68
Special Staining : Flagellar surrounded with flagella
Peritrichous
69
Test for Motility non-staining method for demonstration of presence of flagella
Hanging Drop Method
70
Test for Motility long, proteinaceous filaments for locomotion
Flagella
71
Test for Motility Considered as a virulence factor
Flagella
72
Test for Motility Presence or absence is interpreted as
Presence = motile absence = non-motile
73
Test for Motility used for serotyping
Flagellar antigens
74
Test for Motility : HDM Appearance of bacterial cells
appear translucent with slight greenish color
75
Test for Motility : Hanging Drop Method Specimen
Broth cultures of known motile or non-motile bacteria
76
Test for Motility : HDM True Motility : [movement]
bacterial cells exhibit directional purposeful movement
77
Test for Motility : HDM Non-motile bacteria movement
Brownian movement
78
Test for Motility : HDM random jiggling or shaking due to bombardment of water molecules
Brownian movement
79
Test for Motility : Use of SIM Specimen
Broth cultures of known motile or non-motile bacteria
80
Test for Motility : Use of SIM Medium
Sulfide Indole Medium
81
Test for Motility : Use of SIM Incubation of medium ____ hrs at ____ C
18 - 24 hrs at 35 - 37°C
82
Test for Motility : Use of SIM Growth Pattern : bacterial growth is away from the site of inoculation
Motile
83
Test for Motility : Use of SIM Growth Pattern : bacterial growth is limited to the site of inoculum
Non-motile
84
Test for Motility : Use of SIM Appearance of Motile
Appears diffuse or hazy
85
Test for Motility : Use of SIM Appearance of Non-motile
Surrounding medium is clear
86
Test for Motility : Use of SIM Inoculate culture by making a [action] down the center of the tube about [depth] of the medium
stab using inoculating needle; 3/4 the depth
87
Gram Staining Procedure Reagents + Time
1. Crystal Violet 1 minute 2. Gram's Iodine 1 minute 3. 95% Ethanol or Acetone Alcohol 10 seconds 4. Safranin O or Carbol Fuchsin 30 seconds
88
AFS : Ziehl Neelsen Staining Procedure Reagents + Time
1. Carbol Fuchsin 10 mins 2. Steam 3. Acid Alcohol 3 mins 4. MB / MG less than 1 minute
89
AFS Reason why we use heat after flooding Carbol Fuchsin
to enhance penetration of dye to the cells
90
AFS Reason for using filter paper during staining
to avoid drying out
91
Test for Motility HDM Petroleum jelly is used to
seal cover slip & drop of bacteria; to keep it from drying out
92
Spore Staining Procedure Reagents + Time
1. Malachite Green 10 mins 2. Safranin O 30 seconds
93
Flagellar Staining Procedure Reagents + Time
1. Leifson dye 7-15 mins
94
Test for Motility HDM Slide used
concavity slide
95
Test for Motility HDM Bacterial cells would appear as
translucent with slight greenish color

[3Y1S] MT 6320 (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions