Unit 3.2 BLOOD CELL COUNT Flashcards
Numerical evaluation of formed elements of blood Estimation of the number of blood cells in a known volume of blood.
HEMOCYTOMETRY
Units used in reporting cell counts
Relative Count:
Absolute Count:
%
cells X 10^12/L; cells X 10^9/L; cells /uL
Diluting pipets:
microglass capillary pipets that automatically suck in just the right amount of sample;
Automatic pipets
connected to a plastic container containing just the right amount of sample (Eg. Trenner and Unopette)
Automatic pipets
Non-automatic pipets
Thoma pipets (2BC and RBC diluting pipets)
Red
RBC pipet
White
WBC pipet
Larger bulb
RBC pipet
Smaller bulb
WBC pipet
100
RBC pipet
10
WBC pipet
Smaller bore
RBC pipet
Larger bore
WBC pipet
5; 1; 101
RBC pipet
5; 1; 11
WBC pipet
1:200
RBC pipet
1:20
WBC pipet
1:100-1:1000
RBC pipet
1:10-1:100
WBC pipet
1:10-1:100
1:10
WBC
0.1 - 3?0
1:20
WBC
3.1 - 30.0
1:100
RBC
> 30?0
1:200
RBC
/= 100.0
A counting chamber designed for counting cells.
Hemocytometer
It consists of a thick microscope glass with a rectangular indentation that creates a chamber.
Hemocytometer