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Microbiology > Unit 4: Aerobic GPB > Flashcards

Unit 4: Aerobic GPB Flashcards

(71 cards)

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1
Q

Arcanobacterium haemolyticum: clinical significance?

A
  • Acute pharyngitis similar to Group A strep

- Rash in 50%

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2
Q

Arcanobacterium haemolyticum: Important tests

A
  • Reverse CAMP

- Lecithinase POS

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3
Q

Arcanobacterium haemolyticum: colony morphology

A

Small beta

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4
Q

Bacillus anthracis: colony morphology

A

“medusa head” “egg white” nonhemolytic

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5
Q

Bacillus anthracis: Gram stain

A

Large GPB; boxcar

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6
Q

Bacillus anthracis: clinical significance?

A
  • Potential bioterrorist agent
  • 3 Forms
  • Reservoir: herbivorous animals and spores in soil
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7
Q

Bacillus anthracis: Important tests

A
  • Egg yolk agar POS
  • No growth on PEA
  • Lecithinase POS
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8
Q

Bacillus cereus: colony morphology

A

Green, feathery spreading and Beta

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9
Q

Bacillus cereus: Gram stain

A

Large GPB; boxcar with spores

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10
Q

Bacillus cereus: clinical significance?

A
  • Reservoir: soil, straw & rice
  • Food poisoning
  • Opportunistic infections
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11
Q

Bacillus cereus: Important tests

A
  • Egg yolk agar POS
  • Growth on PEA
  • Lecithinase POS
  • Motility POS
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12
Q

Corynebacterium spp: Gram stain

A

Small GPB palisading, club shaped

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13
Q

Corynebacterium spp: clinical significance?

A

-Typically normal flora of skin and respiratory tract

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14
Q

Corynebacterium diphtheriae: clinical significance?

A
  • Diphtheria toxin
  • Diphtheric pseudomembrane
  • Specimen types: oropharynx and nasopharynx swabs
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15
Q

Corynebacterium diphtheriae: Important tests

A
  • Elek test positive
  • Grows on BAP
  • Loeffler for GS morph (methylene blue)
  • T/C TBA selective for thisbrownish/black colonies
  • Ferments glucose and maltose
  • Nitrate reduction positive
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16
Q

Corynebacterium jeikeium: Colony morphology

A

Gray white nonhemolytic; grows better on blood than choc(lipophilic)

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17
Q

Corynebacterium jeikeium: clinical significance?

A
  • Colonizes skin of hospital patients
  • Infections in immunocompromised hosts
  • Multi drug resistance seen
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18
Q

Corynebacterium jeikeium: Important tests

A

Resistant to P10 disk

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19
Q

Corynebacterium urealyticum: Colony morphology

A

White, pinpoint nonhemolytic and lipophilic

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20
Q

Corynebacterium urealyticum: clinical significance?

A

-urinary pathogen

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21
Q

Corynebacterium urealyticum: important tests

A

-Urease positive

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22
Q

Erysipelothrix rhusiopathiae: Colony morphology

A

Small colonies on BA, greenish coloration but not alpha hemolytic

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23
Q

Erysipelothrix rhusiopathiae: gram stain

A

Slender GPB, can be filamentous

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24
Q

Erysipelothrix rhusiopathiae: clinical significance?

A

Reservoir: Mammals, fish, birds

  • Contact with tissues of infected animals
  • Ingestion of contaminated meat
  • Mild localized cutaneous infection, usually on fingers and hands
  • Specimen types: biopsy, blood cultures
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25
Erysipelothrix rhusiopathiae: important tests
H2S positive
26
Gardnerella vaginalis:Colony morphology
Pinpoint nonhemolytic | May take >24 hrs to grow
27
Gardnerella vaginalis:gram stain
Gram variable bacilli
28
Gardnerella vaginalis: clinical significance?
-Normal flora of female genital tract -Bacterial vaginosis -UTIs in men and women Specimen: genital culture
29
Gardnerella vaginalis:important tests
-Wet prep: >20% clue cells -Whiff test -Grows best with increased CO2 V agar: hemolysis on HBT and purple yellow Sodium Hippurate POS
30
Lactobacillus sp.:Colony morphology
Alpha
31
Lactobacillus sp.: gram stain
Long slender GPB
32
Lactobacillus sp.: clinical significance?
- Normal flora of female genital tract | - Specimens: urine culture
33
Lactobacillus sp.: important tests
Some grow best anaerobically
34
Listeria monocytogenes: Colony morphology
Looks similar to Group B strep, narrow “soft” zone of Beta
35
Listeria monocytogenes: gram stain
GPB or short, plump coccobacilli
36
Listeria monocytogenes: clinical significance?
- Reservoir: soil, water, sewage, vegetation - Biofilm grows well at 4C - Colonized mothers can pass to fetus - Infection of CNS with high mortality rate - Never normal flora in genital and amniotic fluid specimens
37
Listeria monocytogenes: important tests
- Growth at 4C - Sodium Hippurate POS - CAMP test POS block type - Bile esculin POS - Motility POS: tumbling on wet mount and umbrella pattern at RT
38
Rhodococcus spp.(especially equi): Colony morphology
Nonhemolytic, round, sometimes mucoid, often turns pink/orange/red after a few days
39
Rhodococcus spp.(especially equi): gram stain
Diphtheroid like GS
40
Rhodococcus spp.(especially equi): clinical significance?
- Very diverse group | - Infections in immunocompromised patients
41
Rhodococcus spp.(especially equi): important tests
- Partially acid fast | - Needs Rapid ID systems
42
Which species are catalase positive?
Bacillus Corynebacterium Listeria monocytogenes Rhodococcus
43
Which species are catalase negative?
Arcanobacterium Erysipelothrix Lactobacillus Gardnerella vaginalis
44
Which are beta hemolytic?
Arcanobacterium Bacillus cereus Listeria monocytogenes
45
Which are lethicinase positive?
Arcanobacterium | Bacillus spp
46
Which is -Typically normal flora of skin and respiratory tract?
Corynebacterium spp
47
Which are normal flora of female genital tract?
Lactobacillus | Gardnerella vaginalis
48
Which is H2S positive?
Erysipelothrix rhusiopathiae
49
Which one: Small colonies on BA, greenish coloration but not alpha hemolytic?
Erysipelothrix rhusiopathiae
50
filamentous gram stain?
Erysipelothrix rhusiopathiae
51
which one may take >24 hrs to grow
Gardnerella vaginalis
52
which one is Alpha hemolytic?
Lactobacillus sp.
53
Some grow best anaerobically applies to ?
Lactobacillus sp.
54
Which one has this gram stain: GPB or short, plump coccobacilli?
Listeria monocytogenes
55
Biofilm grows well at 4C?
Listeria monocytogenes
56
Colonized mothers can pass to fetus?
Listeria monocytogenes
57
Never normal flora in genital and amniotic fluid specimens?
Listeria monocytogenes
58
Diphtheroid like GS?
Rhodococcus spp.
59
Partially acid fast?
Rhodococcus spp.
60
Which are positive for motility?
Bacillus cereus | Listeria monocytogenes
61
Which is positive for bile esculin?
Listeria monocytogenes
62
Which is positive for CAMP test?
Listeria monocytogenes- block type
63
Which is positive for Sodium hippurate?
Listeria monocytogenes
64
Which is positive for urease?
Corynebacterium urealyticum
65
Which is positive for nitrate reduction?
Corynebacterium diphtheriae
66
Tumbling (wet) or umbrella pattern (RT) motility?
Listeria monocytogenes
67
3 forms of Bacillus anthracis?
Cutaneous anthrax Pulmonary anthrax Gastrointestinal anthrax
68
Most common form of Bacillus anthracis?
Cutaneous anthrax
69
Which form of Bacillus anthracis is associated with Woolsorter’s Disease
Pulmonary anthrax
70
Principle of egg yolk agar?
Lethicinasebreak down lethicin into insoluble diglyceridewhite opaque zone around colony
71
Principle of ELIK test?
Immunodiffusion: precipitation lines form at 45 degree angle if positive

Microbiology (11 decks)

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