Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

3.0 Microbiology > VIVA Qs > Flashcards

VIVA Qs Flashcards

1
Q

MRSA genes

A

mecA and mecC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Most common ESBLs

A

Escherichia coli (E. coli)
Klebsiella pneumoniae.
Pseudomonas aeruginosa.
Proteus mirabilis.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

How would you investigate an ESBL

A

ESBLs are beta-lactamase producing so they are resistant to amoxicillin

Investigated by putting up ampicillin + amp+clav (augmentin)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is augmentin?

A

Ampicillin + clavulanic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is augmentin used for?

A

Treatment of ESBLs like E. Coli or Klebsiella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How would you confirm an ESBL?

A

Put up ampicillin + augementin

ESBL should be resistant to ampicillin

ESBL should be susceptible to augmentin as it contains clavulanic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How would you further investigate a confirmed ESBL?

A

A, B, C, D disc sensitivities to investigate if ESBL is AMPC producing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the 5 CPE genes

A

OXA48 (Dublin)
KPC (WEST)
NDM
VIM
IMP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

CPE Molecular principle

A

Flow Flex PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

BioFire Film Array PCR

A

Nested multiplex PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Roche Light Cycler PCR

A

Real Time PCR

Melting curve analysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Gene Xpert PCR

A

Real-time PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

MALDI-TOF

A

Matrix-assisted laser desorption/ionisation

Time-of-Flight

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

MALDI-TOF principle

A

Matrix added to sample
Sample is irradiated with a laser

ionised sample is separated accordinSg to mass in TOF tube

Pattern of fragments compared to database to ID organism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

SediMax Principle

A

Uses bright-field and phase-contrast microscopy to visualise and recognise formed elements of urine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Enteric Genes (5)

A

C. diff = Toxin A and toxin B
Norovirus = G1 and G2
Campylobacter = tuf gene
Shigella = IpAH
Salmonella = SpaO

17
Q

How to process a sputa

A

Label + book in
Describe appearance
Sputasol for 20 mins
Inoculate plates with 10ul sample
Dilution plate (10 in 20ml distilled water)
Disc blood + chocolate

18
Q

How to process a BAL

A

Sputasol any mucoid samples for 20 min
Must be centrifuged
Pellet goes to TB
Supernatant used to inoculate plates
Separate loop for each plate

19
Q

How to process a CSF

A

3+ samples
Alliqot for bio (glucose + protein)
Serial red cell count
White cell count on last sample
White cell diff if wbc >5
Film array (no gram)
Culture (if bacterial on blood and choc)

20
Q

How to process a sample for TB

A

Any tissues must be mushed up and suspended in water
Sputa/Bals in sputasol for 20 mins
All samples spun for 20 mins
Auramine slide made
Sample spun down again + pour off supernatant
TB buffer solution added to pellet
Mixture used to inoculate TB bottles for BacT

21
Q

How do you make an auramine O slide

A

Make slide with diamond tipped pen
Fix slides @ 70 degrees for 1 hour
Decontaminate slides (sputa/BALS/urines) with NaOH for 20 mins
Neutralise NaOH
Stain with Auramine O

22
Q

When would you put up a geneXpert for TB?

A

If query atypical TB
- auramine slide is positive but BacT is negative

23
Q

Most common organisms in BSIs

A

65% if BSI caused by E. Coli in MMUH (second most common worldwide)
Staphylococcus aures (first most common worldwide)
Strep pneumoniae

GNBs, ents and GPB e.g. diptheroids are also common

Rarely get yeast but they are a very serious infection

24
Q

How long are blood cultures incubated

A

5 days for most
Query endocarditis = 7 days
Query Brucella = 21 days

25
Q

What might cause a positive blood culture but negative subculture

A

High white blood cells
Overfilled bottle
Fastidious organism

26
Q

What would you do with a positive blood culture but negative gram

A

Do a cytospin to condense any present bacteria down

27
Q

What might cause a false negative BC?

A

S. pneumoniae auto-lysis

28
Q

Most common causes of bacterial meningitis in adults

A

H. influenzae
S. pneumoniae
N. meningitidis

29
Q

Most common cause of viral meningitis

A

70% enterovirus

30
Q

Most common cause of meningitis in children

A

Adenovirus

31
Q
A
32
Q

MALDI principle

A

3.0 Microbiology (21 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions