W5 Enzymes Flashcards
(34 cards)
which enzyme cleaves all peptide bond sequences
papain
what does DNA polymerase I do
add nulceotides to template strand
what is an important feature of enzymes functions
specificity
what is Vmax of enzymes
rate of active sites being binded to
maximum saturation value
what does crystallography prove for on enzymes
E/S complexes are being formed
how does spectroscopic data prove E/S complexes
change of absorption and fluorescence of proteins
what are catalytic groups on active site
the amino acid side chains in the active site
what is k+1 for enzymes
forming of enzyme substrate
what is k-1 in enzymes
enzyme + substrate dissociation
what is V0
initial rate of reaction
what does Kcat represent in terms of energy
fully saturated energy
what are the 3 factors that determine Km value
pH
temp
ionic strength
what is the rate determined by
k + 2 constant
from a graph how is Km determined
half of Vmax
what does low Km mean
more products
what happens when the K cat is high
increase rate of reaction
what is enzymes activity limited by
Diffusion rate
whats the lineweaver burk plot
method of calculating Vmax and Km
what is delta G independent of
reaction pathway
what happens to transition state when enzyme reduce activation barrier
becomes lower
what is rate of reaction for small and large delta G
larger delta g is slower reaction
smaller delta g is faster reaction
what do enzymes do for recycling energy
convert wasteful energy into other types of energy
what is the term for delta g negative
exergonic
