W8- CELL WALL Flashcards
(22 cards)
this genus is know to infect or colonize humans and not animal in origin
-they belong to the family Mycoplasmataceae
Mycoplasma and Ureaplasma
it is known to be the smallest free living form. Unlike other bacteria, this prokaryotes doesn’t have a cell wall
-ubiquitous (present everywhere)
-has a 150 diff spp
-
Mycoplasma
What are the most prominent species of the genus Mycoplasma and Ureaplasma
- Mycoplasma pneumoniae
- Mycoplasma hominis
- Ureaplasma urealyticum
General Characteristic of Mycoplasma
- small cell size( 0.3 byt 0.8um) and small genome size
- requires STEROLS for membrane functions and growth
- mostly aerobic
- fastidious
What is the latin word for Class mollicutes
“SOFT SKIN”
this species have been insolated from the genital urinary tract of humans
- Mycoplasma hominis
- Ureaplasma urealyticum
this species have been insolated from the respiratory tract
Mycoplasma pneumoniae
What specie commonly colonizes infants ?
Note: this colonization does not persist beyond the age of 2
- Ureaplasma urealyticum
- mycoplasma hominis
T/F
ones an individual reaches puberty colonization with this mycoplasma can occur primarily as a result of sexual contact
TRUE
Mode of transmission for this agent to cause disease in the humans
-from a colonized mother to her newborn child
What species is the common cause of community acquired pneumonia
Mycoplasma pneumoniae
What are the species of mycoplasma is associated with HIV
- M. fermentans
- M. penetrans
- M. pirum
T/F
Mycoplasma colonize mucosal surfaces of the respiratory and urogenital tract
TRUE
What species has been implicated as playing a possible role in Mongonococcal urethritis
M. genitalium
T/F
since mycoplasma doesn’t have a cell wall, they are highly susceptible to drying therefore, TRANSPORT MEDIA are necessary
TRUE
Note: specially swab specimens
What test is use for the detection of mycoplasma
- PCR (polymerase chain reaction)
- Immunoblotting and Indirect immunoflouroscence
it appears to be highly sensitive and specific test to detect mycoplasma pneumoniae
PCR
it appears SPHERICAL, grainy, yellowish forms that are embedded in the AGAR with a thin outer layer
M. pneumoniae
Appears as DARK BROWNISH CLUMPS
-identified on A8 agar by urease production in the presence of a calcium chloride indicator
U. urealyticum
Appears LARGE with a characteristics “Fried-egg” appearance
- pinpoint colonies
- do not stain with gram stain
- nonhemolytic
- urease negative
- Conventional blood agar
M. Hominis
What is the most widely test use today to M. pneumoniae
ELISA
M. pneumoniae is susceptible to what?
Macrolides and tetracyline antibiotics
