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BACTERIOLOGY > Water Bacteriology > Flashcards

Water Bacteriology Flashcards

(59 cards)

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1
Q

What is Water Microbiology

A
  • deals with the study of microorganisms and parasites in water
  • here in Phil, institutions are analyzing bacteria (limited to) in water only
  • ensure safe consumption of water
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2
Q

Sample used in water microbiology

A
  • Water

not body fulids

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3
Q

3 sections of Water testing laboratories

A

Environmental laboratory
- for metal analysis in water

Wet laboratory
- for the physical and chemical analysis of water

Microbiological Laboratory
- for bacteriological analysis if water

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4
Q

3 types of water dealt with in a water microbiology lab

A
  • drinking water
  • waste water
  • recreational water
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5
Q

Dechlorinating agent used in water microbiology

A

Sodium Thiosulfate

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6
Q

Who are the analyst required to work in water microbiology Lab

A

registered: in DENR

  • medical technologist
  • microbiologist
  • food technologist

In DOH they accept:

  • chemist
  • biologist

must undergo special training by East avenue medical center

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7
Q

Quality of a good drinking water

A
  • clear
  • colorless
  • free from objectionable taste and odor
  • Must not contain any substance or organisms, chemicals, and radioactive materials
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8
Q

Why and when to detect microorganism in water

A

❑ Outbreak investigation of waterborne diseases
❑ Assesing safety, stability of water and water products for public consumption
❑ Determining level of sanitation during product preparation (e.g., in restaurants)
❑ Regulatory compliance (in business)
❑ Incidence surveys

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9
Q

True or False:

In water microbiology specific isolation of the bacteria is necessary

A

False

  • the test only limits to identifying the preence and abscence of Coliform bacteria
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10
Q

are microorganisms such as bacteria and viruses in water bodies, which are utilized as a surrogate to evaluate the presence of pathogens in that environment.

A
  • Indicator organisms or are the COLIFORM bacteria
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11
Q

The most common Coliform (facultative anaerobe) bacteria

A
  • Escherichia coli (most numerous)
  • Enterobacter
  • Klebsiella
  • Citrobacter
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12
Q

True or False:
If the water tested positive with the indicator bactera, it mean that there is also possible presence of all relevant pathogens?

A
  • True (almost 90%)
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13
Q

2 classifications of Coliforms (facultative anaerobe)

A
  • Total Coliform (found everywhere)

- Fecal/ Thermotolerant Coliform (found in fecal material)

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14
Q

Characteristic of Coliforms

A 
❑Facultative anerobe
❑ Gram negative bacilli
❑ Non-spore forming
❑ Ferment lactose at
35oC for 48 hours - (total choliforms)
44.5oC for 24 hours- (Fecal
coliforms)
❑ Non- pathogenic
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15
Q

Bacterial detection Methods

A

❑ DIRECT ENUMERATION
❑ INDIRECT ENUMERATION
❑ PATHOGEN ISOLATION AND IDENTIFICATION

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16
Q

Direct Enumeration can be done through?

A

❑ Microscopic Count

❑ CFU count using non-selective media

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17
Q

Indirect Enumeration can be done through?

A

❑ Most Probable Number (MPN) or Multiple Tube Fermentation Technique

  • quantitative estimation
  • applied in 3 types of water
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18
Q

Pathogen Isolation and Identification can be done through

A

❑ Series of biochemical tests

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19
Q

Acceptable water testing method mandated by Philippine National Standards for Drinking Water. Department of Health. 2017.

A

❑ Multiple Tube Fermentation Technique
❑ Standard Membrane Filtration Technique
❑ Heterotrophic Plate Count (Pour or Spread Plate)
(the above are most common)

❑ Enzyme Substrate Coliform Test
❑ Delayed Incubation Total Coliform Membrane Filter Technique
❑ Simultaneous Detection of Total Coliform and and E.coli by
Dual Chromogen Membrane Filter Technique

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20
Q

Standard Membrane Filtration Technique

and Heterotrophic Plate Count (Pour or Spread Plate) is applied in what type of water

A
  • drinking water

waste water can be done on these methods however, the density of bacteria limits the method.

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21
Q

technique that can be used to classify organisms in all of the 3 types of water

A

Multiple Tube Fermentation Technique

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22
Q

This is where reglations and rules are made or where the submission of requirements in water testing is passed

A

Philippine National Standards for Drinking Water. Department of Health.

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23
Q

Storage of Culture Media in Water microbiology laboratories

A

Store dehydrated media (powder/granulated) in:

  • tightly closed bottles
  • in the dark
  • <30 degrees celcius
  • Low humidity (<50%)
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24
Q

True or False:

If culture medium can still be used even if they become discolored or become
caked and lose the character of a free flowing powder.

Study These Flashcards
A
  • False

do not use them

25
Sterilization of culture media tubes is done through
-Autoclaving at 121 degrees celcius C, 15 psi for 15 minutes. Note: Dispense first before autoclaving Record final pH after autoclaving
26
True or False: Culture media used in Membrane filtration technique can be autoclaved
- False some of their components are carcinogenic are tends to deteriorate
27
Water used in preparation of culture medias in water microbiology lab
- Distilled or deminaralized water
28
True or False: Fermentation tubes can be store in 25 °C for more than 2 weeks.
False - because evaporation may proceed rapidly under these conditions, resulting in marked changes in concentration of the ingredients,
29
What must de done in liquid media fermentation or carbohydrate tubes if they are refrigerated?
- bring in room temperature and discard tubes that contain bubbles
30
What are the labels necessary to be included in the culture media?
❑ Media/Reagent Name ❑ Date prepared ❑ Expiration date ❑ Prepared by
31
Preparation of Culture media plates
- Autoclave first then dispense - 4 to 6 mL into 50 mm x 9 mm petri dishes is used - solidify at room temp. - store in refrigerator at inverted position in a plastic bag or tight container Note: prefreably use for no longer than one week
32
In Multiple Fermentation technique: Presumptive identification is done in what culture media
Lauryl Sulfate Broth or Lauryl Tryptose Broth (LSB/LTB) - for both total and fecal coliforms
33
In Multiple Fermentation technique: Confirmatory identification is done in what culture media
Brilliant Green Lactose Broth (BGLB) - For Total Coliforms detection EC (Escherichia coli) Broth - For Fecal Coliforms detection
34
The accepted dilution water used in Multiple tube fermentation
❑ 0.1 % Peptone water ❑ Stock phosphate buffer solution ❑ Magnesium chloride stock solution
35
True or False: It is not advisable to suspend a sample in any dilution water for more thab 30 mins at RT.
- True since injury, death, or multiplication may occur
36
Principle behind Multiple tube fermentation technique
- Serial Dilution
37
If 99 ± 2.0 mL solution is needed what is the amount of water to be dispensed
100 mL
38
If 9 ± 0.2 mL mL solution is needed what is the amount of water to be dispensed
10 mL
39
How is 0.1 % peptone water prepared?
1 gram of peptone water and 1000 mL of distilled water
40
In MEMBRANE FILTRATION TECHNIQUE: What agar is used for detection of Total coliforms
- ENDO agar 95% ethanol is added
41
In MEMBRANE FILTRATION TECHNIQUE: What agar is used for detection of Fecal coliforms
- Fc agar Sodium hydroxide (0.2N NaOH) and 1% Rosolic acid is added
42
What is added to Fc agar?
Sodium hydroxide (0.2N NaOH) and 1% Rosolic acid
43
In MEMBRANE FILTRATION TECHNIQUE: What agars are used for Escherichia coli detection
- Urea broth | - mTEC agar
44
Inhibitory agents of Endo agar
- basic fuschin and soldium sulfide inhibits gram (+) bacteria and bacteria that are not total coliform
45
Positive result on Endo agar medium
- Pink colonies/ lactose fermenters differentiates bacteria on the basis of lactose fermentation
46
Inhibitory agents of Fc Agar
- bile salts
47
The most common culture medium used in Heterotrophic plate count technique
Plate Count agar - Pour plate or Spread plate method
48
What is the difference between the Pour plate and Spread plate method
- in pour plate the agar is poured on the time of analysis whereas on spread plate method you have to prepare the agar first before analysis.
49
For wastewater and recreational water, what is added on the collection bottle?
2-3 drops 10% sodium thiosulfate ---> then autoclave
50
For drinking water, what is added on the collection bottle?
2-3 drops of 3% Sodium thiosulfate ---> then autoclave
51
For how many minutes will you run the water, when collecting samples in faucets or water dispenser
2-3 minutes ---> heat the mouth or apply sodium hypochlorite ---> run again 2-3 mins---> collect sample
52
Positive control for Total Coliforms
Escherichia coli | Enterobacter aerogenes
53
Negative control for Total coliforms
Staphylococcus aureus | Pseudomonas spp.
54
Positive control for Fecal Coliforms
Escherichia coli
55
Negative control for Fecal coliforms
Enterobacter aerogenes | Streptococcus faecalis
56
How to check purity of culture organism (if it's pure culture)
- Gram staining or thru Eosin methylene blue (EMB agar)
57
What do E.coli looks like in Eosin methylene blu medium
- greenish-metallic chain
58
what should be done in every batch analysis of the same procedure to know the efficiency of the culture media and reagents prepared?
Blank test
59
What agar is used for Air Micro quality check?
- Plate count agar

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