Week 2 Flashcards
(69 cards)
Vmax depends on [?]
[E]
What is Km?
Km = [S] that gives 1/2Vmax
Km is ___ related to binding affinity.
inversely
Kcat (is or isn’t) dependent on the [E].
isn’t
PCR can detect mutations such as:
small insertions (incl. trinucleotide repeat expansions), deletions
This method can detect point mutations that remove or create a restriction site
PCR-RFLP
ARMS-PCR can ONLY detect
point mutations
ASO can detect
small insertions, small deletions, point mutations
This method can detect insertions, deletions and point mutations that remove or create a restriction site, trinucleotide repeat expansions
Southern Blotting
This method is frequently used in identifying triplet repeat expansions
Southern Blotting
Sanger sequencing can detect
deletions, insertions, duplications, and point mutations
This is the only method that can pick up on ALL mutations in a given region
sanger; good for discovering novel mutations
Define caudal
of, at or near the tail or hind parts, posterior
Define Cephalad
towards the head or the anterior part
-tomy
cutting into or incision (arthrotomy - cut into a joint)
-stomy
surgically created opening (colostomy)
-plasty
surgical repair (arthroplasty - surgical reconstruction or repair of a join)
-algia
pain (metatarsalgia - pain under the metatarsal heads)
-osis
abnormal condition (dermatosis - abnormal condition of the skin)
-malacia
abnormal softening (cardiomalacia - softening of the heart tissue)
-megaly
enlargement (oranomegaly - enlargement of the organs)
sagittal plane
left and right (think of side view, both side and sagittal start with “S”)
transverse plane
top and bottom portions
movement occurring at a point in time
-tion (pronation), -xion (dorsiflexion), -sion (inversion), -ing (inverting)
