Flashcards in WHAT IS PDR Deck (19)
What is PDR?
When the pathogens own genome confers a resistance via genetic engineering
What is a pathogen?
an organism causing disease to its host
What does PTGS stand for?
Post transcriptional gene silencing
What is gene induced PTGS?
A resistance to a virus caused by over expressing one of its proteins. This way a part of the virus gets cloned.
What is RNA induced PTGS?
A piece of antisense and a sense one get cloned to produce transcripts
Is similar to a coding fragment. This way it sits on top of the fragment and stops it from getting expressed
The DNA sense strand looks like the messenger RNA (mRNA) transcript, and can therefore be used to read the expected codon sequence
Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA). DNA safely and stably stores genetic material in the nuclei of cells as a reference, or template.
What does "VIGS" mean?
Viral induced gene silencing
How do we obtain a VIGS vector?
A fragment of a target gene is added into a plant virus.
What is VIGS used for?
For fast method screening of target genes
What does screening mean?
It is a method to identify if an organism has a gene or not
What is the perfect VIGS vector?
A virus that can affect a lot of species. The more the merrier.
What can VIGS be also used for?
What are co-infections?
Multiple infection of one host by multiple pathogens
How do we silence with AGO Proteins?
A guide strand is loaded into that protein,
it produces a mRNA that can silence our target gene
What do siRNAs do in silencing?
They are there to enhance the silencing signal.
What does MIGS mean?
miRNA induced gene silencing