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Flashcards in WHAT IS PDR Deck (19)
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1

What is PDR?

When the pathogens own genome confers a resistance via genetic engineering

2

What is a pathogen?

an organism causing disease to its host

3

What does PTGS stand for?

Post transcriptional gene silencing

4

What is gene induced PTGS?

A resistance to a virus caused by over expressing one of its proteins. This way a part of the virus gets cloned.

5

What is RNA induced PTGS?

A piece of antisense and a sense one get cloned to produce transcripts

6

Antisense fragment

Is similar to a coding fragment. This way it sits on top of the fragment and stops it from getting expressed

7

Sense fragment

The DNA sense strand looks like the messenger RNA (mRNA) transcript, and can therefore be used to read the expected codon sequence

8

Transcription

Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA). DNA safely and stably stores genetic material in the nuclei of cells as a reference, or template.

9

What does "VIGS" mean?

Viral induced gene silencing

10

How do we obtain a VIGS vector?

A fragment of a target gene is added into a plant virus.

11

What is VIGS used for?

For fast method screening of target genes

12

What does screening mean?

It is a method to identify if an organism has a gene or not

13

What is the perfect VIGS vector?

A virus that can affect a lot of species. The more the merrier.

14

What can VIGS be also used for?

Co-infections

15

What are co-infections?

Multiple infection of one host by multiple pathogens

16

How do we silence with AGO Proteins?

A guide strand is loaded into that protein,
it produces a mRNA that can silence our target gene

17

What do siRNAs do in silencing?

They are there to enhance the silencing signal.

18

What does MIGS mean?

miRNA induced gene silencing

19

How do we use MIGS?

We add a sequence that the MiRNA recognizes to a transgene. The miRNA will just continue transcribing it then.