LIMITATIONS AND TOOLS OF THIS FIELD Flashcards Preview

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Flashcards in LIMITATIONS AND TOOLS OF THIS FIELD Deck (5)
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1

what does off target mean?

That your modification etc. reaches a target you didn't want it to.

2

Why can RNAi sequence cause degradation of non target mRNAs?

Because it is homologous

3

How much percent of transcripts can be off target when used as a trigger for PTGS?

50-70%

4

Why can off target effects cause biosafety and environmentally issues?

Because it can affect entirely different organisms

5

How can we avoid biosafety and environmentally issues?

By choosing a really specific dsRNA trigger (14 nt is long enough)