White blood cell anatomy and physiology Flashcards
(23 cards)
Do wbcs have a nucleus
Yes
What colour are wbcs
Colourless
They migrate through capillary walls into
Body tissues
Granulocytes- polymorphs are types of
Neutrophils
Eosinophils
Basophils
Agranulocytes- mononuclear involves
Lymphocytes
Monocytes
Romanovsky type stains is a mixture is
A basic dye and an acidic dye- methylene blue and eosin
Used specifically for staining blood smears
Eg. Wrights stain, may grunwald giesma, diff quick stain
Proportions of different types of wbc
Differential wbc count
Percent of eavh type of wbc in a blood sample
Perfect varies depending on species
Granulopoiesis happens in
Bone marrow
Stages of granulopoiesis
- Myeloblast- first recognisable granulocytic cell
- Myelocyte- proliferating mitotic pool
- Metamyelocyte
- Circulating forms blood
Neutrophils- polymorphs features
Approx 12mm diameter
Characteristic segmented nucleus more lobes as the cell matures
Small specific staining granules in cytoplasm
What is a marginating neutrophil
Neutrophil still in circulation
Neutrophils in the blood are divided into two pools
Circulating
Marginating
Extravasation
Neutrophils enter connective tissue by squeezing between endothelial cells lining the blood vessels- diapedesis
Functions of neutrophils within the connective tissue
Attracted to site where there is inflammation
Acts as the 1st line of defence against microbial infection
Active phagocyte/ modulate inflammation and tissue damage
2 types of granules
When are band neutrophils present
If demand is high, immature forms are released from the BM
Horse shoe shaped nucleus is in
Banded neutrophils
Eosinophil features
2-8 percent of total wbc
Typically bi lobed nucleus
Characterisation feature- pink cytoplasmic granules.
Eosinophils take 2-6 days to develop in BM
Circulation exit to tissue
Eosinophil functions
Important in parasitic infections- degranulate in the presence of helminths
Regulated inflammatory and allergic type reactions
Phagocytosis of antigen-antibody complexes
Deactivate histamine and inhibit the release of histamine and seratonin
Basophil features
Rare
Bilobed
Purple- blue granules
Basophil function
Role in inflammatory and allergic type reactions
Contain histamine, heparin and seratonin
Supplement action of mast cells
Monocytes features
Largest type of wbc
3-8 percent total wbc
Variably shaped nucleus
Cytoplasm- greyish
Develop and stored in BM and spleen
Lymphocyte features
Round densely strained nucleus
Sky blue cytoplasm
Size of rbcs
Lymphocyte functions
Cell mediated immune reactions
Antibody production
Antigen recognition
B cell lymphopoiesis is completed in the BM
