wk 2 lab

Question 1

se to prepare
solutions of a specific concentration

Answer 1

Volumetric Flasks

Question 2

measure volumes
of liquid of above 50m

Answer 2

Graduated Cylinders-

Question 3

measure and
deliver volumes ranging from 0.1- 50 mL

Answer 3

Serological Pipettes-

Question 4

• measure and deliver
  volumes ranging from 0.2uL- 1000u

Answer 4

Micropipettes

Question 5

• store reagents and
  samples

Answer 5

Conical tubes

Question 6

microscope that is crucial for studying ultrafine structures like proteins, viruses, and cellular organelles, providing detailed insights into molecular organization and interactions

Answer 6

electron microscope

Question 7

Provides operator and
environment protection but not product protection. It
is suitable for work with low to moderate risk agents

Answer 7

Class I Biosafety Cabinet

Question 8

Offers protection to the operator, environment, and
the product

Answer 8

Class II Biosafety Cabinet (Types A1, A2, B1, B2):

Question 9

: A totally enclosed, gastight cabinet for handling the highest level of
biological agents. It provides maximum protection to
the operator and the environment.

Answer 9

Class III Biosafety Cabinet

Question 10

organize and hold multiple
microcentrifuge tubes during
procedure

Answer 10

MCT RACK

Question 11

maintains a consistent and
controlled temperature for samples,
crucial for incubation and enzyme
reaction

Answer 11

HEAT BLOCK

Question 12

used for incubating samples,
thawing, or heating solutions
in tubes or vials, providing a
controlled and uniform
environment

Answer 12

water bath

Question 13

for nucleic acid purification

Answer 13

spin column

Question 14

used to discard fluids during
nucleic acid extraction

Answer 14

COLLECTION TUBE

Question 15

nucleic acid and protein
quantification, purity assessmen

Answer 15

SPECTROPHOTOMETER

Question 16

designed for efficient and high-throughput extraction of nucleic
acids (DNA or RNA) or other
biomolecules from biological
sample

Answer 16

AUTOMATED EXTRACTION
MACHINe

Question 17

designed to hold samples or
reagents at low temperature

Answer 17

COLD RACKS

Question 18

hold individual reaction tubes in
a compact format, allowing
multiple samples to undergo
PCR simultaneously

Answer 18

PCR STRIPS/ 96-WELL PLATE

Question 19

used to spin down PCR
reaction mixtures, to collect
any liquid from the sides of the
tubes and ensure proper pellet
formation

Answer 19

PCR CENTRIFUGE

Question 20

for reagent preparation
It provides a controlled environment to set up PCR reactions, handle PCR
products, and prevent contaminatio

Answer 20

UV CABINET

Question 21

a device used for the Polymerase Chain Reaction (PCR).

Answer 21

THERMOCYCLER

Question 22

It facilitates the precise and automated
cycling of temperature to amplify and replicate DNA or RNA sequences.

Answer 22

THERMOCYCLER

The thermocycler’s ability to
control temperature changes enables repeated cycling through specific temperature phases,
including denaturation, annealing, and extension, necessary for PCR amplification

Question 23

works by displacing the air from the pipette shaft and
allow the liquid to be drawn into the resulting vacuum

Answer 23

Micropipette

Question 24

Dispensing the liquid by pressing the plunger to the first stop and then releasing
it

Answer 24

1.Forward Pipetting

Question 25

Aspirating a slightly larger volume than needed (2nd stop), and then dispensing the desired volume by releasing the plunger to the first sto

Answer 25

Reverse Pipetting

Question 26

Aspirating and dispensing the liquid multiple times in the same container to mix the contents thoroughly.

Answer 26

Mixing Pipetting

Question 27

Using a pipette with multiple channels to pipette several samples simultaneously, increasing efficiency.

Answer 27

Multichannel Pipetting

Question 28

Sequentially diluting a sample by transferring a portion to a new container, typically used for concentration adjustments

Answer 28

Serial Dilution