wk 5

Question 1

: ability to separate nucleic acids
based on their size or other properties

Answer 1

Resolution

Question 2

: ability to visualize or identify
nucleic acids after separation

Answer 2

Detection

Question 3

are the most
practical and commonly used methods

Answer 3

Gel and capillary electrophoresis

Question 4

DNA can also be spotted and detected using

Answer 4

specific hybridization probes

Question 5

refers to the movement of
molecules under an electric current.

Answer 5

Electrophoresis

Question 6

charge properties

Answer 6

phosphate is negative

dna and rna is positive

Question 7

Used in bacterial culture dishes and electrophoresis.

Answer 7

AGAROSE GELS

Question 8

what does high and low concentration of agarose gel indicate or do

Answer 8

Higher concentration: Resolves smaller DNA fragments.
Lower concentration: Resolves larger DNA fragments.

Question 9

Used for very large DNA fragments

Answer 9

PULSED-FIELD GEL ELECTROPHORESIS (PFGE)

Question 10

Alternates positive and negative electrodes.

Answer 10

Field-Inversion Gel Electrophoresis (FIGE) 1.

Question 11

Applies uniform field strength.

Answer 11

Contour-Clamped Homogeneous Electric Field (CHEF) 2.

Question 12

Uses transverse reorientation.

Answer 12

Transverse Alternating-Field Electrophoresis (TAFE) 3.

Question 13

Rotates the gel for better separation.

Answer 13

Rotating Gel Electrophoresis (RGE) 4.

Question 14

Used for small DNA fragments, RNA, and proteins.

Answer 14

POLYACRYLAMIDE GELS

Question 15

Commonly used for:
DNA sequencing.
Mutation analysis.
Nuclease protection assays.

Answer 15

POLYACRYLAMIDE GELS

Question 16

is widely used for separating organic and inorganic
substances.

Answer 16

Capillary electrophoresis (CE)

Question 17

Used in pharmaceuticals, carbohydrates, metal ions, and nucleic acid analysis.

Answer 17

CAPILLARY ELECTROPHORESIS