wk 2 media prep

Question 1

TYPES OF CULTURE

Answer 1

1. pure
2. mixed
3. stock

Question 2

 It is composed of only one specie

Answer 2

pure culture

Question 3

 It is composed of more than one species

Answer 3

MIXED CULTURE

Question 4

Composed of several specie contained in a separate culture
medium

 Used for academic or individual purpose

Answer 4

STOCK CULTURE

Question 5

A nutrient material prepared for the growth of
microorganisms in a laboratory

Answer 5

CULTURE MEDIA

Question 6

COMMON INGREDIENTS OF CULTURE MEDIA

Answer 6

• water, peptone, agar, blood serum, malt extract, yeast extract

Question 7

yeast extract

Answer 7

Amino acids, growth factors,
inorganic salts

Question 8

malt extract

Answer 8

Maltose, starchy, dextrins, and
glucose, 5% of proteins and
protein breakdown products,
mineral salts and growth factors

Question 9

BLOOD/SERUM

Answer 9

Enriching Culture media

Question 10

Digested protein

Answer 10

peptone

Question 11

CLASSIFICATION OF CULTURE MEDIA

Answer 11

CONSISTENCY
COMPOSITION
FORMATION
FUNCTION

Question 12

Nutrients are dissolved in water and bacterial growth is
indicated by a change in broth’s appearance from clear to
turbid

Answer 12

LIQUID MEDIA

Question 13

 Made by adding a solidifying agent to the nutrients and water

Answer 13

SOLID MEDIA

Question 14

For special purposes where agar is added to media in
concentrations that are too low to solidify them

Answer 14

SEMI-SOLID MEDIA

Question 15

 Composition is exactly known, quantitatively. They are
chemically defined media prepared from pure chemical
substances. (e.g. SCA, MGM)

Answer 15

SYNTHETIC

Question 16

Has a known composition only with approximation. The
complex medium contains ingredients in unknown
proportions (e.g. TSA, NA)

Answer 16

NON-SYNTHETIC

Question 17

The medium test the ability of
organisms to utilize citrate as the
sole carbon source

Answer 17

Simmons Citrate Agar

Question 18

This mineral medium is the first to
be published synthetic medium
for Listeria that has been shown
to be strictly carbon limited

Answer 18

Mineral Glucose Medium

Question 19

General purpose, non-selective
growth medium that supports the
growth of most non-fastidious
bacteria

Answer 19

Trypto-casein Soy Agar

Question 20

Simple medium that supports the
growth of many organisms

Answer 20

Nutrient Agar

Question 21

Used for obligate intracellular bacteria (Rickettsia and
chlamydia)

Answer 21

TISSUE

Question 22

ACCORDING TO FORMATION

Answer 22

 Slant
 Slant/Deep
 Deep
 Broth

Question 23

Contains nutrients that support the growth of nonfastidious
organisms without giving any particular organism a growth
advantage

Answer 23

NUTRITIVE

Question 24

When a substance is added to a liquid medium which inhibits
the growth of unwanted bacteria and favor the growth of
unwanted bacteria

Answer 24

ENRICHMENT

Question 25

nutritive agar example

Answer 25

 E.g. Nutrient Agar, Trypto-casein Soy Agar, SDA

Question 26

ENRICHMENT agar example

Answer 26

 E.g. Selenite F, Alkaline Peptone Water, Tetrathionate broth

Question 27

Are prepared to meet the nutritional requirements of more exacting bacteria by the addition of substances such as blood, serum, or egg to the basal medium

Answer 27

enriched

Question 28

enriched media example

Answer 28

 E.g. BAP, CAP, LSS, BCYE

Question 29

Contains one or more agents that are inhibitory to all organisms except those “selected” by the specific growth condition or chemical

Answer 29

SELECTIVE

Question 30

selective agar example

Answer 30

 E.g. MAC, TCBS, LJ, PEA, DCA, MTM

Question 31

Employ some factor that allow colonies of bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing in the same plate

Answer 31

DIFFERENTIAL

Question 32

DIFFERENTIAL MEDIA EXAMPLE

Answer 32

 E.g. MAC, BAP, HEA, XLD

Question 33

Is a holding medium designed to preserve the viability of organisms by maintaining moist environment and giving nutrients but will not allow multiplication

Answer 33

TRANSPORT MEDIA

Question 34

TRANSPORT MEDIA EXAMPLE

Answer 34

JEMBEC/TRANSGROW, Stuarts transport medium, Cary-Blair

Question 35

These media are used to grow anaerobic organisms and contain reducing substances

Answer 35

ANAEROBIC

Question 36

ANAEROBIC MEDIA EXAMPLE

Answer 36

 E.g. Thioglycolate Broth, Cooked Meat Broth

Question 37

 Used for antibiotic susceptibility testing

Answer 37

MHA

Question 38

shiny opaque, convex, white colonies 2-3 mm in diameter after 48hrs

Answer 38

S AUEREUS

Question 39

Organisms that uses light as energy source

Answer 39

PHOTOTROPH

Question 40

Organisms that utilize the energy produced by organic or inorganic compound oxidation

Answer 40

CHEMOTROPH

Question 41

TWO TYPES OF CHEMOTROPH

Answer 41

Autotroph/Litotroph AND Heterotroph/Organotroph

Question 42

Organisms requiring oxygen for growth

Answer 42

OBLIGATE AEROBES

Question 43

Organisms NOT requiring oxygen for growth

Answer 43

OBLIGATE ANAEROBES

Question 44

Organisms not requiring air but enhanced in its presenc

Answer 44

FACULTATIVE ANAEROBE

Question 45

MICROAEROPHILE

Answer 45

Organisms requiring small amount of air

Question 46

Do not grow well but survives in the presence of air

Answer 46

AEROTOLERANT

Question 47

Grows between pH 0 – 5.5

Answer 47

ACIDOPHILIC

Question 48

Grow between 8.5 – 11.6

Answer 48

BASOPHILIC/ALKALOPHILIC

Question 49

Grows between pH of 7.0 – 7.5

Answer 49

NEUTROPHILIC