bacte lab spcl > staph and stre[[ > Flashcards

staph and stre[[ Flashcards

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1
Q

culturing on blood agar plates, growing as round, smooth, white (sometimes yellowish) and beta-hemolytic (completely lyses RBCs)

A

staphylococcus

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2
Q

To differentiate Staphylococa, Micrococaus, and Streptococaus

A

CATALASE TEST

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3
Q

Transfer a small amount of colony into a clean glass slide then drop 3% H2o2

A

CATALASE TEST

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4
Q

result in catalse test

A

RESULT
(+) Continuous Bubbling
(-) No or few bubbles are formed

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5
Q

positive and negative example in catalase

A

QUALITY CONTROL
(*) Control = Staphylococcus aureus
(-) Control = Streptococcus pyogenes

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6
Q

To differentiate Staphylococcus aureus from other StaphylococcoI spp.

A

COAGULASE TEST

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7
Q

an enzyme that reacts with fibrinogen to for a clot

A

coagulase

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8
Q

SLIDE coagulase method

A

: Place a drop of rabbit’s plasma (EDTA) onto a slide then emulsify a loopful of bacteria to create a suspension. Rock the slide for 5-10 seconds and observe for agglutination

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9
Q

TUBE method

A

: emulsify a loopful of bacteria in 0.5ml of rabbit’s plasma and incubate in 37°C for 4 hours. Observe for clot formation every 30 mins

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10
Q
  • detects clumping factors in the surface of bacterial cells.
A

COAGULASE- SLIDE METHOD

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11
Q
  • Fibrinogen → clumping factor → Fibrin
A

COAGULASE- SLIDE METHOD

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12
Q

Detects thrombin-like molecule called coagulase reacting factor (CRF).

A

COAGULASE- TUBE METHOD

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13
Q

extracellular molecule that will react with CRF

A

Staphylocoagulase

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14
Q
  • Fibrinogen→ coagulase-CRF complex → Fibrin
A

COAGULASE- TUBE METHOD

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15
Q

PURPOSE To differentiate Staphylococc from Micrococci

A

MODIFIED OXIDASE TEST

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16
Q

Bacteria that possess cytochrome C produce a dark-blue end product when reacted with Modified Oxidase rgt

A

MODIFIED OXIDASE TEST

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17
Q

result of microdase

A

RESULT
(+) Development of purple-blue color
(-) No Color change

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18
Q

positive and negative control for microdase test

A

(+) Control = Micrococous luteus
(-) Control = Staphylococeus aureus

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19
Q

Lysostaphin

A

Lysostaphin (200 ug/mL)
> Staphylococcus (S) ; Micrococcus (R)

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20
Q

Lysosome R AND S

A

Lysosome (50 mg disk)
Micrococcus - S
Staphylococcus - R

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21
Q

BACITRACIN S AND R

A

Bacitracin (0.04U)
Micrococcus - S
Staphylococcus - R

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22
Q

NovobiocinS AND R

A

S
S. aureus ; S. epidermidis

R
S. saprophyticus

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23
Q

Furazolidone S AND R

A

Furazolidone (100 ug)
Staphylococcus
Micrococcus

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24
Q

WHICH IS THE GLUCOSE FERMENTER AND OXIDIZER BETWEEN STAPH AND MICROCOCCUS

A

STAPH -FERMENTER
MICRO- OXIDIZER

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25
STREP FOUND IN GENITAL TRACT AND OCCASIONAL COLONIZER OF URT
S. AGALACTIAE
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strep in oral caity, GI tract, female genital tract
s. bovis
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normal flora of gi tract, and female genital tract
enteroccocus faecalis
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not a normal flora, skin urt
s pyogenes
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causes rheumatic fever, carlet fever, pharyngitis, glomerulonephritis, pyogenic infection
s pyogenes
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neonatal sepsis, meningitis and [uereral fever
s. agalactiae
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pneumonia, meningitis, ottitis media
s pneumoniae
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endocarditis and uti
s bovis
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nosocomial infection and uti
enterococcus faecalis
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* Small, transparent, smooth, sometimes beta-hemolytic
streptococus
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o2 requirement of strep
The Species of Streptococci are facultative anaerobe. Some are capnophilic
36
how m
37
any hours do you incubatestrep
18-48 hours
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* Grayish white, transparent to translucent, matte or glossy with large zone of hemolysis
Group A Streptococci
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* Larger than GAS, translucent to opaque. Flat colonies, glossy with narrow zone of beta-hemolysis.
Group B Streptococci
40
Small, gray, glistening umbilicated colonies (might resemble a doughnut). May appear mucous if polysaccharide producing strain. Alpha hemolytic
Pneumococci
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Minute to small, translucent, gray, Dome-shaped or umbonate center. Alpha hemolytic or non hemolytic
Viridans
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- Small, Cream or white colored colonies. Smooth, entire margin. Alpha, Beta, or non-hemolytic
Enterococcus
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partial lysis of rbc around colony
alpha hemolytic
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greenish discoloration of area around colony
beta hemolysis
45
no lysis around colony
nonhemolytic
46
small area of intact rbc around colony or no change in agar
nonhemolytic
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small area of intact rbc around colony
alpha prime (a') or wide zone
48
alpha hemolytic in respiratory tract
viridians
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respiratory tract, pathogenic
s pneumoniae
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alpha hemolytiv in gi tract
enterococcus
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beta hemolytic strep in gi tract
pyogenes
52
genitourinary tract, b hemolytic
s agalactiae
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drug very useful for treating superficial skin infections but it is too toxic for systemic use;
Bacitracin
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bacitracin is a polypeptide antibiotic produced by
Bacillus subtilis
55
Interferes with the peptidoglycan synthesis of bacteria
bacitracin
56
To differentiate GAS from other Beta-hemolytic streptococci.
BACITRACIN SUSCEPTIBILITY
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taxo a test result
RESULT Susceptible -Zone of inhibition > 10mm Resistant- No zone of inhibition
58
positive and negative control in taxo a
QUALITY CONTROL Susceptible Control = Streptococcus pyogenes Resistant Control = Streptococcus agalactiae
59
Interferes ATPase and ATP production.
60
aka optochin
ethyl hydrocupreine hydrochloride (Optochin)
61
To determine the effect of ethyl hydrocupreine hydrochloride
taxo p or optochin susceptibility
62
sensitive to optochin
Pneumococci is sensitive while other alpha hemolytic streptococci are not
63
optochin test result
RESULT Susceptible - Zone of Inhibition 214mm Resistant - No zone of inhibition
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result remark in optochin
Results that are <1.4mm, must be confirmed by doing bile solubility
65
CAMP (
Christie, Atkins, and Munch- Petersen)
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To differentiate GBS from other streptococcal specie.
CAMP
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that acts synergistically to beta hemolysins produced by s. aureus.
CAMP factor
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RESULT OF CAMP
RESULT (+) Presence of enhanced hemolysis (arrow head hemolysis near the Juncture point of the organism - Streptococcus agalactiae (-) no enhanced hemolysis - Streptococcus pyogenes
69
test cAn also be used for Listeria monocytogenes
CAMP
70
hydrolyzed by the enzyme hippuricase into glycine and Benzoic acid
hippurate
71
Glycine will be deominated by ninhydrin reagent to from a purple colored product.
Hippurate hydrolysis
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Hippurate hydrolysis procedure
PROCEDURE Make a bacterial suspension in Iml of sterile water then place a hippurate disk. Incubate for 2 hrs then add 0.2 ml ninhydrin reagent. Reincubate for 15-30 min and observe for color development
73
hippurate hydrolysis result
RESULT (+) Deep purple color (-) Colorless or yellow-pink color
74
positive and negative control in hippurate hydrolysis
QUALITY CONTROL (+) Control = Streptococcus agalactiae (ATCC| 2386) (-) Control = Streptococcus pyogenes (ATCC19615)
75
False positive result may occur if incubated more than 30 min
hipppurate hydrolysis
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For presumptive identification of GAS and enterococci,
L- pyrrolidonyl Arylamidase (PYR) TEST
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result in pyr
(+) Bright Red Color (-) No color change / orange color
78
To determine the ability of an organism to grown in high salt environment
SALT TOLERANCE TEST
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SALT TOLERANCE TEST result
RESULT (+) Visible turbidity w/ or w/o color change (Purple to yellow) (-) No turbidity and no color change.
80
Presumptive Identification of Enterococci and Streptococcus bovis group.
BILE ESCULIN TEST PURPOSE:
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are Inhibited by bile salts.
Gram positive bacteria, besides Streptococcus bovis and Enterococcus,
82
result of be test
RESULT (+) Growth and Blackening of the agar slant (-) No growth and no blackening of the agar
83
positive and negative control in slat tolerance test
v+) Control = Enterococcus faecalis (-) Control = Escherichia coli
84
Steps in laboratory identification
* Perform Direct examination first * Gm(+) cocci → Perform Catalase * If Catalase (+) → Proceed to Staphylococcal ID * If Catalase (-) → Proceed to Streptococcal ID * For Streptococcal ID, observe for Hemolysis

bacte lab spcl (4 decks)

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