Filoviridae

Question 1

Filo means?

Answer 1

threadlike

Question 2

Filoviridae is genetically similar to?

Answer 2

Paramyoxviridae and rhabdoviridae

Question 3

Name the two genera of Filoviridae

Answer 3

• Ebola like virus
  -Marburg like virus

Question 4

Name the 5 strains of Ebola

Answer 4

• African strains: Zaire, Sudan, Ivory
  Coast, Uganda
• Pilipino strain: Reston

Question 5

Describe the morphology and structure of Filoviridae

• ____________
• ___________, Enveloped or non-enveloped?
• 80 nm diameter
• 130-140 nm long
• 19 kb in length
• _______-stranded, _________, non-__________
• ___- Ve sense RNA
• appears to have “spikes” due to _____________ on outside membrane

Answer 5

• Pleomorphic
• Filamentous, Enveloped
• 80 nm diameter
• 130-140 nm long
• 19 kb in length
• single-stranded, linear, non-segmented
• -Ve sense RNA
• appears to have “spikes” due to glycoprotein
  on outside membrane

Question 6

Answer 6

Ebola

Question 7

Answer 7

Marburg

Question 8

Answer 8

?

Question 9

Describe the Ebola replication cycle

Answer 9

Attachment
internalization
tranascription
translation
maturation
release
Endosome –> exchanges –> release of NA into cytoplasm –> genome cut –> released outside of cell

Question 10

Ecology of Ebola Virus

Answer 10

Transmission from bat to another animal b/c bats usually live near other animals.
From those species, it jumps to others, including humans.

Question 11

Describe the pathogenesis of Ebola

Answer 11

• Enters bloodstream
  – skin, membranes, open wounds
• Cell Level
  – docks with cell membrane
• Viral RNA
  – released into cytoplasm
  – production new viral proteins/ genetic material
• New viral genomes
  – rapidly coated in protein
  – create cores

Question 12

Describe the early symptoms observed in a patient infected with Ebola virus

Answer 12

• Early symptoms
  – muscle aches, fever, vomiting
  – red eyes, skin rash, diarrhea, stomach pain

Question 13

Describe the acute symptoms observed in a patient infected with Ebola virus

Answer 13

• Acute symptoms
  – bleeding/hemorrhaging from the skin, orifices, internal organs

Question 14

Describe the early diagnosis made in a patient infected with Ebola virus

Answer 14

• Early Diagnosis
• very difficult
• signs & symptoms very similar to other infections

Question 15

What diagnostic tests would you perform if you suspect your patient is infected with Ebola?

Answer 15

• Laboratory Test
• PCR detection
• ELISA (enzyme-linked immuno-absorbant) assay

Question 16

How can you prevent the spread of Ebola virus?

Answer 16

• No vaccines!
• Patients are isolated
• Medical Staff Training
  – western sanitation practices
• intake
• care during stay
• after patient dies
• Infection-control Measures
  – complete equipment and area sterilization

Question 17

How do you process the body of a patient infected with Ebola virus?

Answer 17

After Death
Virus contagious in fluids for days
* Burial use extreme caution
– handling and transport
– cultural practices/ religious belief
– Incinerate all waste !!!!