Dehydration

Question 1

Step in tissue processing that involves removal of intra and extracellular water from tissue.

Answer 1

Dehydration

Question 2

Temperature that will hasten dehydration time.

Answer 2

37degC

Question 3

Commonly used in dehydration, fast acting and nontoxic.

Answer 3

Alcohol

Question 4

How is alcohol used as a dehydrating agent?

Answer 4

Use it in increasing or ascending concentration (70%→80%→90%)

Question 5

What will happen if a dehydrating agent is used in decreasing concentration?

Answer 5

tissue will be damaged

Question 6

Most commonly used alcohol for dehydration process.

Answer 6

Ethyl Alcohol

Question 7

Alcohol for plant and animal micro technique.

Answer 7

Butyl Alcohol

Question 8

What is the purpose of phenol in alcohol bath?

Answer 8

It acts as a tissue softener (for nails etc.)

Question 9

Used for dehydrating sections and smears.

Answer 9

Triethyl Phosphate

Question 10

Dehydrating agent that is toxic by ingestion, inhalation and by skin contact.

Answer 10

Cellosolve (Ethylene Glycol Monoethyl Ether)

Question 11

Highly corrosive on skin and is toxic to reproductive, fetal and urinary system.

Answer 11

Cellosolve (Ethylene Glycol Monoethyl Ether)

Question 12

Cellosolve decomposes when exposed to ________.

Answer 12

sunlight

Question 13

Can dehydrate and clear tissue at the same time but not commonly used.

Answer 13

Dioxane/Diethylene Dioxide

Question 14

Dehydrating agent whose main disadvantage is its being toxic.

Answer 14

Dioxane/Diethylene Dioxide

Question 15

Dehydrating agent that is both a skin and eye irritant.

Answer 15

THF (Tetrahydrofuran)

Question 16

Dehydrating agent that has an offensive odor – therefore, the room should be well ventilated.

Answer 16

THF (Tetrahydrofuran)

Question 17

Dehydrating agent that is rapid acting and is suited for urgent biopsies but not for routine tissue processing (fixative and dehydrating agent).

Answer 17

Acetone