Clearing

Question 1

Removal of alcohol from tissue acquired from dehydration process.

Answer 1

Clearing/ dealcoholization

Question 2

True or False. Many reagents we use for dealcoholization process can make tissues transparent thus it is also called clearing; but not all can make tissue transparent.

Answer 2

True

Question 3

Routinely used clearing agent but not for nervous tissue and lymph node.

Answer 3

Xylene

Question 4

Can become milky after placing an incompletely dehydrated tissue on it.

Answer 4

Xylene

Question 5

Clearing agent that can damage the bone marrow and may cause aplastic anemia.

Answer 5

Benzene

Question 6

What are the 2 clearing agents that can be easily removed from tissues causing shortened infiltration time.

Answer 6

Benzene and Xylene

Question 7

Can be used as xylene and benzene substitute.

Answer 7

Toluene

Question 8

Clearing agent that is toxic to liver.

Answer 8

Chloroform

Question 9

Clearing agent that is recommended for skin, fibroid and decalcified tissues for tough tissue, nervous tissues, lymph nodes, embryos.

Answer 9

Chloroform

Question 10

Clearing agent that will not make tissues transparent.

Answer 10

Chloroform

Question 11

Clearing agent that can attack rubber seal used in vacuum

Answer 11

Chloroform

Question 12

Clearing agent that has the same property as chloroform.

Answer 12

Carbon tetrachloride

Question 13

Clearing agent that can become milky because of prolonged storage.

Answer 13

Cedarwood Oil

Question 14

Clearing agent for CNS tissues & cytological studies, an extremely slow clearing agent.

Answer 14

Cedarwood Oil

Question 15

2 clearing agents that can be used for dense tissues like uterus.

Answer 15

Chloroform and Cedarwood Oil

Question 16

True or False. Clearing agents that are difficult to remove from tissue accelerates infiltration time.

Answer 16

False. It prolongs infiltration time.

Question 17

For clearing insects, embryos and other delicate specimens.

Answer 17

Aniline Oil

Question 18

When this clearing agent is used, tissue becomes adulterated.

Answer 18

Clove Oil

Question 19

Recommended for eye specimen.

Answer 19

Terpineol

Question 20

Clearing agent obtained from citrus fruit with a faint pleasant odor.

Answer 20

Limonene

Question 21

What are the steps in tissue processing that will require complete clearing of tissues?

Answer 21

Infiltration and mounting