Dehydration Flashcards
Removal of intracellular and extracellular water from the fixed tissue in preparation for impregnation procedure
Dehydration
Widely used dehydrating agent in the lab
Alcohol
Recommended ration of dehydrating agent to tissue
10:1
Done after fixation and prior to wax impregnation
Dehydration
Characteristics of an ideal dehydrating ageng
- Dehydrate fast
- NOT Evaporate fast
- Evaporate fatty tissues
- NOT harden the tissue excessively
- NOT remove stains
- NOT toxic to the body
- NOT a fire hazard
Use for routine dehydration
Ethyl alcohol (ethanol)
Clear, colorless and flammable
Ethyl alcohol (ethanol)
Best dehydrating agent
Ethyl alcohol (ethanol)
Ethanol is fast acting because is mixes with water and many organic solvent and penetrates the tissue easily (true or false)
True
Not poisonous and not very expensive
Ethyl alcohol (ethanol)
Substitute for ethanol
Isopropyl alcohol
Dehydrating agent for blood and tissue films
Methyl alcohol
Methyl alcohol is a toxic dehydrating agent (true or false)
True
Fixative for smear preparations (bone marrow)
Methyl alcohol
Utilize in plants and animal micro-techniques
Butyl alcohol
A slow dehydrating agent and is recommended for non-urgent works and it also produces less shrinkage and hardening
Butyl alcohol
The strength of the initial alcohol required will depend upon the size, nature of the tissue and the fixative used. (True or false)
True
Recommended starting concentration of alcohol in delicate tissues
30%